Abstract
Carrot (Daucus carota L.) cell suspensions were treated with a spirostanol saponin from Yucca. This saponin is an elicitor of callose synthesis. Irrespectively of the mode of action of spirostanol on the callose synthase activity itself, the spirostanol-induced callose synthesis in carrot is not preceded by changes in membrane potential, cytosolic free calcium or cytosolic pH. The inability of modulators of cytosolic free calcium content (verapamil, nifedipine and Br-A23187), EGTA and a proton pump inhibitor (vandate) to inhibit or induce callose formation is consistent with a calcium- and pH-independent mechanism for callose deposition.
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Claims 4
Claim · model-written summary
Spirostanol saponin elicited callose synthesis in carrot cells.
- Taxon
- Carrot
Daucus carotae0 - Treatment
- spirostanol saponine0
- Outcome
- callose synthesise0
- Direction
- elicitore0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Carrot (Daucus carota L.) cell suspensions were treated with a spirostanol saponin from Yucca. This saponin is an elicitor of callose synthesis.”
Claim · model-written summary
Spirostanol-induced callose synthesis was not preceded by changes in membrane potential, cytosolic calcium, or cytosolic pH.
- Taxon
- carrotDaucus carotae0
- Treatment
- spirostanole0
- Outcome
- changes in membrane potential, cytosolic free calcium or cytosolic pHe0
- Direction
- not precedede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the spirostanol-induced callose synthesis in carrot is not preceded by changes in membrane potential, cytosolic free calcium or cytosolic pH”
Claim · model-written summary
Calcium modulators, EGTA, and a proton pump inhibitor did not inhibit or induce callose formation.
- Taxon
- CarrotDaucus carotat0
- Treatment
- modulators of cytosolic free calcium content (verapamil, nifedipine and Br-A23187), EGTA and a proton pump inhibitor (vandate)e0
- Outcome
- callose formatione0
- Direction
- inabilitye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The inability of modulators of cytosolic free calcium content (verapamil, nifedipine and Br-A23187), EGTA and a proton pump inhibitor (vandate) to inhibit or induce callose formation”
t0“Carrot Cells”
Claim · model-written summary
The findings support a calcium- and pH-independent mechanism for callose deposition.
- Taxon
- CarrotDaucus carotat0
- Outcome
- callose depositione0
- Qualifier
- calcium- and pH-independent mechanisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“consistent with a calcium- and pH-independent mechanism for callose deposition”
t0“Carrot Cells”
The same questions in other papers
calcium atom · molecular: metabolites · Daucus carota (carrot)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.