Abstract
Here we reported a novel protein elicitor from Bacillus amyloliquefaciens NC6 induced systemic resistance (ISR) in tobacco. The purification was executed by ion-exchange chromatography, native-page extraction and HPLC, and the amino acid sequence was identified by mass spectrometry. This recombinant elicitor protein, expressed in Escherichia coli by an E1 expression vector, had good thermal stability, and the elicitor caused a clearly defined hypersensitive response (HR) necrosis in tobacco leaves. It could also trigger early defence events, including generation of reactive oxygen species (H2O2 and O2 (-)) and phenolic-compound accumulation. Quantitative real-time PCR (Q-RT-PCR) results indicated that several plant defence genes, including the salicylic acid (SA)-responsive PR1a, PR1b, PR5, and phenylalanine ammonia lyase (PAL), as well as the jasmonic acid (JA)-responsive PDF1.2 and CORONATINE INSENSITIVE 1 (COI1), were all up-regulated. Moreover, infiltration conferred systemic resistance against a broad spectrum of pathogens, including Tobacco mosaic virus (TMV) and the fungal pathogen Botrytis cinerea.
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Claims 8
Claim · model-written summary
A novel protein elicitor from Bacillus amyloliquefaciens NC6 induced systemic resistance in tobacco.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- a novel protein elicitor from Bacillus amyloliquefaciens NC6e0
- Outcome
- systemic resistance (ISR)e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here we reported a novel protein elicitor from Bacillus amyloliquefaciens NC6 induced systemic resistance (ISR) in tobacco.”
Claim · model-written summary
The recombinant elicitor protein had good thermal stability.
- Outcome
- good thermal stabilitye0
Not reportedTaxonDirection
Verbatim
e0“This recombinant elicitor protein, expressed in Escherichia coli by an E1 expression vector, had good thermal stability”
Claim · model-written summary
The elicitor caused clearly defined hypersensitive-response necrosis in tobacco leaves.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- leavese0
- Treatment
- elicitore0
- Outcome
- hypersensitive response (HR) necrosise0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the elicitor caused a clearly defined hypersensitive response (HR) necrosis in tobacco leaves”
Claim · model-written summary
The elicitor triggered reactive oxygen species generation and phenolic-compound accumulation in tobacco.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- PeBA1t1
- Outcome
- generation of reactive oxygen species (H2O2 and O2 (-)) and phenolic-compound accumulatione0
- Direction
- triggere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It could also trigger early defence events, including generation of reactive oxygen species (H2O2 and O2 (-)) and phenolic-compound accumulation.”
t0“Tobacco”
t1“PeBA1”
Claim · model-written summary
The elicitor upregulated tobacco PR1a, PR1b and PR5 defence genes.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- PeBA1t1
- Outcome
- PR1a, PR1b, PR5e0
- Direction
- up-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Quantitative real-time PCR (Q-RT-PCR) results indicated that several plant defence genes, including the salicylic acid (SA)-responsive PR1a, PR1b, PR5, and phenylalanine ammonia lyase (PAL), as well as the jasmonic acid (JA)-responsive PDF1.2 and CORONATINE INSENSITIVE 1 (COI1), were all up-regulated.”
t0“Tobacco”
t1“PeBA1”
Claim · model-written summary
The elicitor upregulated tobacco PAL, PDF1.2 and COI1 defence genes.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- PeBA1t1
- Gene
- PDF1.2e0
- Outcome
- phenylalanine ammonia lyase (PAL)e0
- Direction
- up-regulatede0
- Qualifier
- CORONATINE INSENSITIVE 1 (COI1)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“phenylalanine ammonia lyase (PAL), as well as the jasmonic acid (JA)-responsive PDF1.2 and CORONATINE INSENSITIVE 1 (COI1), were all up-regulated.”
t0“Tobacco”
t1“PeBA1”
Claim · model-written summary
Elicitor infiltration conferred systemic tobacco resistance against Tobacco mosaic virus.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- infiltratione0
- Interactor
- Tobacco mosaic virus (TMV)
Tobacco mosaic viruse0pathogen - Outcome
- systemic resistancee0
- Direction
- conferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Moreover, infiltration conferred systemic resistance against a broad spectrum of pathogens, including Tobacco mosaic virus (TMV) and the fungal pathogen Botrytis cinerea.”
t0“Tobacco”
Claim · model-written summary
Elicitor infiltration conferred systemic tobacco resistance against Botrytis cinerea.
- Taxon
- TobaccoNicotiana tabacumt0
- Treatment
- infiltratione0
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Outcome
- systemic resistancee0
- Direction
- conferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Moreover, infiltration conferred systemic resistance against a broad spectrum of pathogens, including Tobacco mosaic virus (TMV) and the fungal pathogen Botrytis cinerea.”
t0“Tobacco”
The same questions in other papers
Bacillus amyloliquefaciens · disease · Nicotiana tabacum (tobacco)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
resistance inducer · disease · Nicotiana tabacum (tobacco)Results agree
12 papers in the same direction as this paper
- positive
- positive
- positive
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.