Abstract
The capacity of H(2)O(2), the most stable of the reactive oxygen species (ROI), to diffuse freely across biological membranes and to signal gene expression suggests that H(2)O(2) could function as a short-lived second messenger diffusing from cell to cell. We tested this hypothesis in tobacco plants treated with a glycoprotein elicitor. Applied at 50 nM, it induces H(2)O(2) accumulation and the hypersensitive response restricted to the infiltrated zone 1 tissue. Stimulation of a set of defense responses also occurs in the surrounding zone 2 tissue without diffusion of the elicitor. ROI levels in zone 1 were modulated using N-acetyl-L-cysteine (NAC) as a ROI scavenger and Rose Bengal (RB) as a ROI generator. We found that ROI appeared to act as signalling intermediates in pathways leading to salicylic acid accumulation, to PR1, PR5 and 3-hydroxy-3-methylglutarylCoA reductase expression in glycoprotein-treated zone 1 tissues. Compared to the treatment with the elicitor alone, co-infiltration of the glycoprotein and NAC increased the surface of zone 2 showing PR1 and O-methyltransferase expression. Application of RB had the opposite effect. The data suggest that, in our system, ROI did not act as a cell-to-cell diffusible signal to activate PR protein and O-methyltransferase expression in zone 2.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Glycoprotein elicitor induced hydrogen peroxide accumulation and localized hypersensitive response.
- Taxon
- tobacco
Nicotiana tabacume0 - Organ / stage
- zone 1 tissuee0
- Treatment
- glycoprotein elicitore0
- Outcome
- H(2)O(2) accumulation and the hypersensitive responsee0
- Direction
- inducese0
- Value
- 50 nMe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We tested this hypothesis in tobacco plants treated with a glycoprotein elicitor. Applied at 50 nM, it induces H(2)O(2) accumulation and the hypersensitive response restricted to the infiltrated zone 1 tissue.”
Claim · model-written summary
Defense responses were stimulated in surrounding tissue without elicitor diffusion.
- Taxon
- tobaccoNicotiana tabacume1
- Organ / stage
- zone 2 tissuee0
- Outcome
- Stimulation of a set of defense responsese0
- Qualifier
- without diffusion of the elicitore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stimulation of a set of defense responses also occurs in the surrounding zone 2 tissue without diffusion of the elicitor.”
e1“tobacco plants treated with a glycoprotein elicitor”
Claim · model-written summary
Reactive oxygen intermediates appeared to signal salicylic acid accumulation in elicitor treated zone 1 tissue.
- Taxon
- tobaccoNicotiana tabacume1
- Organ / stage
- zone 1 tissuese0
- Compound
- ROIe0
- Outcome
- salicylic acid accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ROI appeared to act as signalling intermediates in pathways leading to salicylic acid accumulation, to PR1, PR5 and 3-hydroxy-3-methylglutarylCoA reductase expression in glycoprotein-treated zone 1 tissues.”
e1“tobacco plants treated with a glycoprotein elicitor”
Claim · model-written summary
Reactive oxygen intermediates appeared to signal PR1 and PR5 expression in elicitor treated zone 1 tissue.
- Taxon
- tobaccoNicotiana tabacume1
- Organ / stage
- zone 1 tissuese0
- Compound
- ROIe0
- Outcome
- PR1, PR5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ROI appeared to act as signalling intermediates in pathways leading to salicylic acid accumulation, to PR1, PR5 and 3-hydroxy-3-methylglutarylCoA reductase expression in glycoprotein-treated zone 1 tissues.”
e1“tobacco plants treated with a glycoprotein elicitor”
Claim · model-written summary
Reactive oxygen intermediates appeared to signal reductase expression in elicitor treated zone 1 tissue.
- Taxon
- tobaccoNicotiana tabacume1
- Compound
- ROIe0
- Outcome
- 3-hydroxy-3-methylglutarylCoA reductase expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ROI appeared to act as signalling intermediates in pathways leading to salicylic acid accumulation, to PR1, PR5 and 3-hydroxy-3-methylglutarylCoA reductase expression in glycoprotein-treated zone 1 tissues.”
e1“tobacco plants treated with a glycoprotein elicitor”
Claim · model-written summary
Adding NAC to elicitor increased the zone 2 area expressing PR1 and O-methyltransferase.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- co-infiltration of the glycoprotein and NACe0
- Gene
- PR1 and O-methyltransferasee0
- Outcome
- surface of zone 2 showing PR1 and O-methyltransferase expressione0
- Direction
- increasede0
- Comparator
- treatment with the elicitor alonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Compared to the treatment with the elicitor alone, co-infiltration of the glycoprotein and NAC increased the surface of zone 2 showing PR1 and O-methyltransferase expression.”
e1“tobacco plants treated with a glycoprotein elicitor”
Claim · model-written summary
Rose Bengal application had the opposite effect on zone 2 defense expression area.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- RBe0
- Outcome
- surface of zone 2 showing PR1 and O-methyltransferase expressione2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Application of RB had the opposite effect.”
e1“tobacco plants treated with a glycoprotein elicitor”
e2“increased the surface of zone 2 showing PR1 and O-methyltransferase expression”
Claim · model-written summary
Reactive oxygen intermediates did not act as a diffusible cell to cell signal for zone 2 defense expression.
- Taxon
- tobaccoNicotiana tabacume1
- Compound
- ROIe0
- Outcome
- PR protein and O-methyltransferase expression in zone 2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ROI did not act as a cell-to-cell diffusible signal to activate PR protein and O-methyltransferase expression in zone 2.”
e1“tobacco plants treated with a glycoprotein elicitor”
The same questions in other papers
glycoprotein · disease · Nicotiana tabacum (tobacco)Results agree
2 papers in the same direction as this paper
- positive
- positive
resistance inducer · disease · Nicotiana tabacum (tobacco)Results agree
12 papers in the same direction as this paper
- positive
- positive
- positive
9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.