Abstract
A new protein elicitor, PeaT1, was purified from the mycelium of Alternaria tenuissima by column chromatography. PeaT1 was identified as a heat‐stable and acidic protein. It induced systemic acquired resistance to tobacco mosaic virus (TMV) in tobacco plants but did not cause hypersensitive response. The elicitor‐encoding gene was cloned by rapid amplification of cDNA ends method. Sequence analysis revealed that the cDNA is 624 bp in length and the open reading frame encodes for a polypeptide of 207 amino acids with a nascent polypeptide‐associated complex domain. The peaT1 gene was cloned into the expression vector pET‐28a and transformed into Escherichia coli BL21 (DE3). The recombinant elicitor also triggered defence responses in intact tobacco plants. The availability of the pure protein offers the possibility to isolate the corresponding receptor and links it to the downstream signalling pathway.
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Claims 7
Claim · model-written summary
PeaT1 was purified from Alternaria tenuissima mycelium.
- Taxon
- tobacco
Nicotiana tabacumt0 - Interactor
- Alternaria tenuissimaAlternaria tenuissimae0pathogen
- Gene
- PeaT1e0
- Outcome
- purified from the mycelium of Alternaria tenuissimae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A new protein elicitor, PeaT1, was purified from the mycelium of Alternaria tenuissima”
t0“tobacco”
Claim · model-written summary
PeaT1 was identified as heat-stable and acidic.
- Taxon
- tobaccoNicotiana tabacumt0
- Gene
- PeaT1e0
- Outcome
- heat‐stable and acidic proteine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PeaT1 was identified as a heat‐stable and acidic protein”
t0“tobacco”
Claim · model-written summary
PeaT1 induced systemic acquired resistance to TMV in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- PeaT1e1
- Interactor
- tobacco mosaic virus (TMV)
Tobacco mosaic viruse0pathogen - Outcome
- systemic acquired resistance to tobacco mosaic virus (TMV)e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It induced systemic acquired resistance to tobacco mosaic virus (TMV) in tobacco plants”
e1“A new protein elicitor, PeaT1, was purified”
Claim · model-written summary
PeaT1 did not cause a hypersensitive response in tobacco.
- Taxon
- tobaccoNicotiana tabacume1
- Treatment
- PeaT1e2
- Outcome
- hypersensitive responsee0
- Direction
- did not causee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but did not cause hypersensitive response”
e1“It induced systemic acquired resistance to tobacco mosaic virus (TMV) in tobacco plants”
e2“A new protein elicitor, PeaT1, was purified”
Claim · model-written summary
The cloned elicitor cDNA was 624 bp long.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- cDNAe0
- Value
- 624 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the cDNA is 624 bp in length”
t0“tobacco”
Claim · model-written summary
The elicitor open reading frame encoded a 207-amino-acid polypeptide with a nascent polypeptide-associated complex domain.
- Taxon
- tobaccoNicotiana tabacumt0
- Outcome
- polypeptidee0
- Value
- 207 amino acidse0
- Qualifier
- nascent polypeptide‐associated complex domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the open reading frame encodes for a polypeptide of 207 amino acids with a nascent polypeptide‐associated complex domain”
t0“tobacco”
Claim · model-written summary
Recombinant elicitor triggered defense responses in intact tobacco plants.
- Taxon
- tobaccoNicotiana tabacume0
- Treatment
- recombinant elicitore0
- Outcome
- defence responsese0
- Direction
- triggerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The recombinant elicitor also triggered defence responses in intact tobacco plants”
The same questions in other papers
resistance inducer · disease · Nicotiana tabacum (tobacco)Results agree
12 papers in the same direction as this paper
- positive
- positive
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See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.