Abstract
The effects of desiccation and storage temperature on the viability and longevity of willow seeds was investigated using two hybrids, Salix rehderiana x (Salix x capreola) [cross 458] and Salix x sericans x Salix viminalis [cross 512]. Freshly harvested seed of both crosses survived silica gel drying down to c. 3 to 5% moisture content. Hybrid 458 seed stored in liquid nitrogen (-196C) for 3 d retained viability when equilibrated to < or = 45% RH (pre-storage), showed slightly reduced survival at 65% RH and exhibited no survival at > or =82% RH. The level of survival after 68 d for seeds pre-equilibrated to either c. 10 or 65% (5 or 10% moisture) and stored at four temperatures was -196C > -20C > 2C > 16C. At all temperatures, drier seed stored better than wetter seed. For hybrid 512, seed longevity at 20C > 40C > 60C, and a 10% fall in pre-storage seed RH resulted in a c. 2-fold increase in longevity at each storage temperature. The response of hybrid willow seeds to desiccation and cooling raises possibilities for the long-term seed conservation of Salix species by cryopreservation.
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Claims 9
Claim · model-written summary
Both willow crosses survived drying to about 3 to 5% moisture.
- Taxon
- Salix hybridst0
- Organ / stage
- seede0
- Treatment
- silica gel dryinge0
- Outcome
- survivede0
- Value
- c. 3 to 5% moisture contente0
Not reportedComparatorDirection
Verbatim
e0“Freshly harvested seed of both crosses survived silica gel drying down to c. 3 to 5% moisture content”
t0“Salix hybrids”
Claim · model-written summary
Hybrid 458 retained viability after three days in liquid nitrogen when equilibrated to at most 45% RH.
- Taxon
- Salix hybridst0
- Material
- Hybrid 458e0
- Organ / stage
- seede0
- Treatment
- stored in liquid nitrogen (-196C) for 3 de0
- Outcome
- retained viabilitye0
- Qualifier
- < or = 45% RHe0
Not reportedComparatorDirection
Verbatim
e0“Hybrid 458 seed stored in liquid nitrogen (-196C) for 3 d retained viability when equilibrated to < or = 45% RH (pre-storage)”
t0“Salix hybrids”
Claim · model-written summary
Hybrid 458 survival was slightly reduced at 65% RH.
- Taxon
- Salix hybridst0
- Material
- Hybrid 458e1
- Organ / stage
- seede1
- Treatment
- 65% RHe0
- Outcome
- survivale0
- Direction
- reducede0
Not reportedComparator
Verbatim
e0“showed slightly reduced survival at 65% RH”
e1“Hybrid 458 seed stored in liquid nitrogen (-196C) for 3 d”
t0“Salix hybrids”
Claim · model-written summary
Hybrid 458 had no survival at at least 82% RH.
- Taxon
- Salix hybridst0
- Material
- Hybrid 458e1
- Organ / stage
- seede1
- Treatment
- > or =82% RHe0
- Outcome
- no survivale0
Not reportedComparatorDirection
Verbatim
e0“exhibited no survival at > or =82% RH”
e1“Hybrid 458 seed stored in liquid nitrogen (-196C) for 3 d”
t0“Salix hybrids”
Claim · model-written summary
After 68 days, hybrid 458 survival ranked -196C above -20C, 2C and 16C.
- Taxon
- Salix hybridst0
- Material
- Hybrid 458e1
- Organ / stage
- seede1
- Treatment
- four temperaturese0
- Outcome
- level of survivale0
- Qualifier
- -196C > -20C > 2C > 16Ce0
Not reportedComparatorDirection
Verbatim
e0“The level of survival after 68 d for seeds pre-equilibrated to either c. 10 or 65% (5 or 10% moisture) and stored at four temperatures was -196C > -20C > 2C > 16C”
e1“Hybrid 458 seed”
t0“Salix hybrids”
Claim · model-written summary
Drier willow seed stored better than wetter seed across temperatures.
Verbatim
e0“At all temperatures, drier seed stored better than wetter seed”
t0“Salix hybrids”
Claim · model-written summary
Hybrid 512 seed longevity ranked 20C above 40C above 60C.
- Taxon
- Salix hybridst0
- Material
- hybrid 512e0
- Organ / stage
- seede0
- Treatment
- 20Ce0
- Outcome
- seed longevitye0
- Qualifier
- 20C > 40C > 60Ce0
- Comparator
- 40Ce0
Not reportedDirection
Verbatim
e0“For hybrid 512, seed longevity at 20C > 40C > 60C”
t0“Salix hybrids”
Claim · model-written summary
A 10% reduction in pre-storage RH approximately doubled hybrid 512 longevity at each storage temperature.
Verbatim
e0“a 10% fall in pre-storage seed RH resulted in a c. 2-fold increase in longevity at each storage temperature”
e1“For hybrid 512, seed longevity”
t0“Salix hybrids”
Claim · model-written summary
The authors suggest cryopreservation for long-term conservation of Salix seeds.
- Outcome
- long-term seed conservation of Salix species by cryopreservatione0
SalixOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“raises possibilities for the long-term seed conservation of Salix species by cryopreservation”
The same questions in other papers
nitrogen · growth · Salix (willows)Positive and negative results
3 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
2 papers more in this group
See all 6 papers →storage · growth · Salix (willows)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.