Abstract
High frequency somatic embryogenesis was directly obtained from stem and leaf explants of cotton abnormal seedling. Direct induction of cotton somatic embryogenesis was affected by different concentrations and combinations of plant growth regulators. Modified MS medium supplemented with 0.1 mg/L IAA and 0.1 mg/L ZT was the best medium for direct induction of high frequency somatic embryogenesis from abnormal seedlings. With this medium, embryogenic callus was obtained within 10 days of culture, mature embryos was obtained within 20～30 days of culture, regenerative plant was obtained within 60 days of culture. For cotton somatic embryogenesis, there was no difference between stem and leaf explants, but difference existed among genotypes. This method was employed in cotton genetic transformation.
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Claims 7
Claim · model-written summary
Somatic embryogenesis was obtained directly at high frequency from abnormal cotton seedling stem and leaf explants.
- Taxon
- cotton
Gossypium hirsutume0 - Organ / stage
- stem and leaf explantse0
- Outcome
- High frequency somatic embryogenesise0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High frequency somatic embryogenesis was directly obtained from stem and leaf explants of cotton abnormal seedling”
Claim · model-written summary
Modified MS medium with 0.1 mg/L IAA and 0.1 mg/L ZT was best for direct high frequency embryogenesis.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Modified MS medium supplemented with 0.1 mg/L IAA and 0.1 mg/L ZTe0
- Outcome
- high frequency somatic embryogenesise0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Modified MS medium supplemented with 0.1 mg/L IAA and 0.1 mg/L ZT was the best medium for direct induction of high frequency somatic embryogenesis from abnormal seedlings”
t0“Cotton”
Claim · model-written summary
Embryogenic callus formed within 10 days on that medium.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- this mediume0
- Outcome
- embryogenic calluse0
- Value
- within 10 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“With this medium, embryogenic callus was obtained within 10 days of culture”
t0“Cotton”
Claim · model-written summary
Mature embryos formed within 20～30 days on that medium.
- Taxon
- CottonGossypium hirsutumt0
- Outcome
- mature embryose0
- Value
- within 20～30 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mature embryos was obtained within 20～30 days of culture”
t0“Cotton”
Claim · model-written summary
Regenerated plants formed within 60 days on that medium.
- Taxon
- CottonGossypium hirsutumt0
- Outcome
- regenerative plante0
- Value
- within 60 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“regenerative plant was obtained within 60 days of culture”
t0“Cotton”
Claim · model-written summary
Cotton somatic embryogenesis did not differ between stem and leaf explants but differed among genotypes.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- stem and leaf explantse0
- Outcome
- somatic embryogenesise0
- Direction
- no differencee0
- Qualifier
- difference existed among genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“For cotton somatic embryogenesis, there was no difference between stem and leaf explants, but difference existed among genotypes”
Claim · model-written summary
The method was used in cotton genetic transformation.
- Taxon
- cottonGossypium hirsutume0
- Outcome
- cotton genetic transformatione0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This method was employed in cotton genetic transformation”
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See all 39 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.