Abstract
Abstract Callus was induced from the micropylar end of cultured cotton ( Gossypium hirsutum L.) ovules by (2‐chloroethyl)phosphonic acid (CEPA). The influence of other phytohormones on the growth of this callus was examined with in vitro culture techniques. Abscisic acid inhibited the callus growth induced by 10 −4 M CEPA, but at low concentrations of abscisic acid and CEPA, callus growth was stimulated slightly. Indoleacetic acid inhibited the callus growth at optimum CEPA concentration, but not when the CEPA level was supraoptimum. Kinetin stimulated micropylar callus growth when ovules were obtained from parent plants grown under long, warm days but not when ovules were from plants grown under short days and cooler temperatures. Gibberellic acid (GA) caused the greatest callus response. Increasing concentrations of GA up to 5 × 10 −7 M markedly stimulated callus growth while increasing the sensitivity of the tissue to CEPA. In addition to induction and growth of the micropylar callus, combinations of CEPA and GA caused a complete release of normal tissue differentiation in the cotton ovule with rapid proliferation of the entire ovule integument. This is a promising system with which to study hormonal control of tissue differentiation.
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Claims 11
Claim · model-written summary
CEPA induced callus from the micropylar end of cotton ovules.
- Taxon
- cotton
Gossypium hirsutume0 - Organ / stage
- ovulese0
- Treatment
- (2‐chloroethyl)phosphonic acid (CEPA)e0
- Outcome
- Calluse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Callus was induced from the micropylar end of cultured cotton ( Gossypium hirsutum L.) ovules by (2‐chloroethyl)phosphonic acid (CEPA).”
Claim · model-written summary
Abscisic acid inhibited CEPA-induced callus growth.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Abscisic acide0
- Outcome
- callus growthe0
- Direction
- inhibitede0
- Qualifier
- 10 −4 M CEPAe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abscisic acid inhibited the callus growth induced by 10 −4 M CEPA”
t0“Cotton”
Claim · model-written summary
Low abscisic acid and CEPA stimulated callus growth slightly.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- low concentrations of abscisic acid and CEPAe0
- Outcome
- callus growthe0
- Direction
- stimulatede0
- Qualifier
- slightlye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at low concentrations of abscisic acid and CEPA, callus growth was stimulated slightly.”
t0“Cotton”
Claim · model-written summary
Indoleacetic acid inhibited callus growth at optimal CEPA.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Indoleacetic acide0
- Outcome
- callus growthe0
- Direction
- inhibitede0
- Qualifier
- optimum CEPA concentratione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Indoleacetic acid inhibited the callus growth at optimum CEPA concentration”
t0“Cotton”
Claim · model-written summary
Indoleacetic acid did not inhibit growth at supraoptimal CEPA.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Indoleacetic acide0
- Outcome
- callus growthe0
- Qualifier
- CEPA level was supraoptimume0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Indoleacetic acid inhibited the callus growth at optimum CEPA concentration, but not when the CEPA level was supraoptimum.”
t0“Cotton”
Claim · model-written summary
Kinetin stimulated callus growth in ovules from long, warm day plants.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Kinetine0
- Outcome
- micropylar callus growthe0
- Direction
- stimulatede0
- Qualifier
- long, warm dayse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Kinetin stimulated micropylar callus growth when ovules were obtained from parent plants grown under long, warm days”
t0“Cotton”
Claim · model-written summary
Kinetin did not stimulate callus growth in ovules from short, cool day plants.
- Taxon
- CottonGossypium hirsutumt0
- Outcome
- ovulese0
- Qualifier
- short days and cooler temperaturese0
Not reportedTreatmentComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not when ovules were from plants grown under short days and cooler temperatures.”
t0“Cotton”
Claim · model-written summary
GA caused the greatest callus response.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Gibberellic acid (GA)e0
- Outcome
- callus responsee0
- Direction
- greateste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gibberellic acid (GA) caused the greatest callus response.”
t0“Cotton”
Claim · model-written summary
Increasing GA up to 5 × 10 −7 M stimulated callus growth.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- GA up to 5 × 10 −7 Me0
- Outcome
- callus growthe0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing concentrations of GA up to 5 × 10 −7 M markedly stimulated callus growth”
t0“Cotton”
Claim · model-written summary
GA increased tissue sensitivity to CEPA.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- GAe0
- Outcome
- sensitivity of the tissue to CEPAe0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Increasing concentrations of GA up to 5 × 10 −7 M markedly stimulated callus growth while increasing the sensitivity of the tissue to CEPA.”
t0“Cotton”
Claim · model-written summary
CEPA plus GA induced proliferation across the ovule integument.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- combinations of CEPA and GAe0
- Outcome
- rapid proliferation of the entire ovule integumente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“combinations of CEPA and GA caused a complete release of normal tissue differentiation in the cotton ovule with rapid proliferation of the entire ovule integument.”
The same questions in other papers
indole-3-acetic acid · growth · Gossypium hirsutum (cotton)Results differ
2 papers in the same direction as this paper · 12 in the opposite direction
- positive
- positive
- positive
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.