Abstract
The browning and death of calli would reduce the efficiency of cotton plant regeneration and genetic transformation,and it was pivotal to inhibit the two peaks of browning and death of calli during cotton tissue culture to improve plant regeneration and genetic transformation efficiency.In this study,we analyzed subculture time,browning death degree,regeneration time and regeneration efficiency of calli of Jihe 713and Coker 312 cultivars.The results showed that subculture time was able to regulate two peaks of browning and death of calli.To take an extended subculture time during the first PCD peak,subculture time interval 50 d can significantly reduce callus browning and death,increase the rate of callus embryogenesis and shorten the time of embryogenesis.To Take a shortened time of subculture during the second PCD peak,subculture interval 15d can significantly reduce the degree of browning and death,thereby increasing the plant regeneration rate and reducing the regeneration time of the plants.Regulation browning and death by callus subculture time will significantly improve the efficiency of genetic transformation in two cotton varieties,so as to help the gene function and genomics research in cotton.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 6
Claim · model-written summary
A 50 d first-peak subculture interval reduced callus browning and death.
- Taxon
- Cotton
Gossypium hirsutumt0 - Treatment
- subculture time interval 50 de0
- Outcome
- callus browning and deathe0
- Direction
- reducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“subculture time interval 50 d can significantly reduce callus browning and death,increase the rate of callus embryogenesis and shorten the time of embryogenesis.”
t0“Different Subculture Time Affects Two Cell Death Peaks in Tissue Culture and Plantlet Regeneration in Cotton”
Claim · model-written summary
A 50 d first-peak subculture interval increased callus embryogenesis rate.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- subculture time interval 50 de0
- Outcome
- rate of callus embryogenesise0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“subculture time interval 50 d can significantly reduce callus browning and death,increase the rate of callus embryogenesis and shorten the time of embryogenesis.”
t0“Different Subculture Time Affects Two Cell Death Peaks in Tissue Culture and Plantlet Regeneration in Cotton”
Claim · model-written summary
A 50 d first-peak subculture interval shortened embryogenesis time.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- subculture time interval 50 de0
- Outcome
- time of embryogenesise0
- Direction
- shortene0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“subculture time interval 50 d can significantly reduce callus browning and death,increase the rate of callus embryogenesis and shorten the time of embryogenesis.”
t0“Different Subculture Time Affects Two Cell Death Peaks in Tissue Culture and Plantlet Regeneration in Cotton”
Claim · model-written summary
A 15d second-peak subculture interval reduced callus browning and death.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- subculture interval 15de0
- Outcome
- degree of browning and deathe0
- Direction
- reducee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“subculture interval 15d can significantly reduce the degree of browning and death,thereby increasing the plant regeneration rate and reducing the regeneration time of the plants.”
t0“Different Subculture Time Affects Two Cell Death Peaks in Tissue Culture and Plantlet Regeneration in Cotton”
Claim · model-written summary
A 15d second-peak subculture interval increased plant regeneration rate.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- subculture interval 15de0
- Outcome
- plant regeneration ratee0
- Direction
- increasinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“subculture interval 15d can significantly reduce the degree of browning and death,thereby increasing the plant regeneration rate and reducing the regeneration time of the plants.”
t0“Different Subculture Time Affects Two Cell Death Peaks in Tissue Culture and Plantlet Regeneration in Cotton”
Claim · model-written summary
A 15d second-peak subculture interval reduced plant regeneration time.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- subculture interval 15de0
- Outcome
- regeneration timee0
- Direction
- reducinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“subculture interval 15d can significantly reduce the degree of browning and death,thereby increasing the plant regeneration rate and reducing the regeneration time of the plants.”
t0“Different Subculture Time Affects Two Cell Death Peaks in Tissue Culture and Plantlet Regeneration in Cotton”
The same questions in other papers
in vitro · growth · Gossypium hirsutum (cotton)Positive and negative results
29 papers in the same direction as this paper · 9 in the opposite direction
- negative
- negative
- negative
35 papers more in this group
See all 39 papers →in vitro · disease · Gossypium hirsutum (cotton)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.