Paper · 2008

Effects of mutagenic treatments on plant regeneration of cotyledon explants in Brassica napus L.

Zhou Wei-jun

9
claims
18
verbatim quotes
0
citations
1
research areas
—
Not open access

Abstract

Effects of ultraviolet(UV) irradiation,mutagenic agents ethylmethane sulphonate(EMS) and sodium azide(NaN3) on plant regeneration of cotyledons in two Brassica napus cultivars(Zheshuang 758 and Zheshuang 6) in vitro were investigated.Results showed that UV had some promoting effects on cotyledon regenerations.The highest rates of callus induction and plant regeneration were observed from the two cultivars when treated with UV for 120 s and 60 s,respectively.EMS at different concentrations significantly affected the numbers of shoot explants and plant regeneration,and there was interactive effect between EMS concentrations and treatment times.When EMS concentration was 20 mg/L and treatment time was 6 h,the highest numbers of shoot explants and plant regeneration were reached.There were significant differences in numbers of regenerated plants between different treatment concentrations of NaN3(500 μmol/L or 100 μmol/L)and between different treatment times(5 h or 24 h).No obvious interactive effect was found among different treatment factors.

Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher.

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 9

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

UV irradiation had promoting effects on cotyledon regeneration in Brassica napus.

Taxon
Brassica napusBrassica napuse0
Organ / stage
cotyledone1
Treatment
UVe1
Outcome
cotyledon regenerationse1
Direction
promotinge1

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“on plant regeneration of cotyledons in two Brassica napus cultivars(Zheshuang 758 and Zheshuang 6) in vitro”

✓ located verbatim in the source · abstract, characters 108–215 · show in text ↑
e1

“Results showed that UV had some promoting effects on cotyledon regenerations”

✓ located verbatim in the source · abstract, characters 234–310 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

UV treatment for 120 s gave the highest callus induction rate in the two Brassica napus cultivars.

Taxon
Brassica napusBrassica napuse0
Material
Zheshuang 758 and Zheshuang 6e0
Treatment
UV for 120 se2
Outcome
callus inductione2
Direction
higheste2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“on plant regeneration of cotyledons in two Brassica napus cultivars(Zheshuang 758 and Zheshuang 6) in vitro”

✓ located verbatim in the source · abstract, characters 108–215 · show in text ↑
e2

“The highest rates of callus induction and plant regeneration were observed from the two cultivars when treated with UV for 120 s and 60 s,respectively”

✓ located verbatim in the source · abstract, characters 311–461 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

UV treatment for 60 s gave the highest plant regeneration in the two Brassica napus cultivars.

Taxon
Brassica napusBrassica napuse0
Material
Zheshuang 758 and Zheshuang 6e0
Treatment
UV for 120 s and 60 se2
Outcome
plant regeneratione2
Direction
higheste2
Qualifier
respectivelye2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“on plant regeneration of cotyledons in two Brassica napus cultivars(Zheshuang 758 and Zheshuang 6) in vitro”

✓ located verbatim in the source · abstract, characters 108–215 · show in text ↑
e2

“The highest rates of callus induction and plant regeneration were observed from the two cultivars when treated with UV for 120 s and 60 s,respectively”

✓ located verbatim in the source · abstract, characters 311–461 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

EMS at 20 mg/L for 6 h gave the highest numbers of shoot explants and plant regeneration.

Taxon
Brassica napusBrassica napuse0
Treatment
EMS concentration was 20 mg/L and treatment time was 6 he3
Outcome
numbers of shoot explants and plant regeneratione3
Direction
higheste3

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“on plant regeneration of cotyledons in two Brassica napus cultivars(Zheshuang 758 and Zheshuang 6) in vitro”

✓ located verbatim in the source · abstract, characters 108–215 · show in text ↑
e3

“When EMS concentration was 20 mg/L and treatment time was 6 h,the highest numbers of shoot explants and plant regeneration were reached”

✓ located verbatim in the source · abstract, characters 650–785 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

EMS at different concentrations significantly affected the numbers of shoot explants and plant regeneration.

Taxon
Brassica napusBrassica napuse0
Treatment
EMS at different concentrationse4
Outcome
numbers of shoot explants and plant regeneratione4

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“on plant regeneration of cotyledons in two Brassica napus cultivars(Zheshuang 758 and Zheshuang 6) in vitro”

✓ located verbatim in the source · abstract, characters 108–215 · show in text ↑
e4

“EMS at different concentrations significantly affected the numbers of shoot explants and plant regeneration,and there was interactive effect between EMS concentrations and treatment times”

✓ located verbatim in the source · abstract, characters 462–649 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

EMS concentrations and treatment times interacted in their effect on the numbers of shoot explants and plant regeneration.

Taxon
Brassica napusBrassica napuse0
Treatment
EMS concentrations and treatment timese4
Outcome
numbers of shoot explants and plant regeneratione4

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“on plant regeneration of cotyledons in two Brassica napus cultivars(Zheshuang 758 and Zheshuang 6) in vitro”

✓ located verbatim in the source · abstract, characters 108–215 · show in text ↑
e4

“EMS at different concentrations significantly affected the numbers of shoot explants and plant regeneration,and there was interactive effect between EMS concentrations and treatment times”

✓ located verbatim in the source · abstract, characters 462–649 · show in text ↑
Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

NaN3 at 500 and 100 μmol/L significantly differed in the number of regenerated plants.

Taxon
Brassica napusBrassica napuse0
Treatment
NaN3(500 μmol/L or 100 μmol/L)e5
Outcome
numbers of regenerated plantse5

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“on plant regeneration of cotyledons in two Brassica napus cultivars(Zheshuang 758 and Zheshuang 6) in vitro”

✓ located verbatim in the source · abstract, characters 108–215 · show in text ↑
e5

“There were significant differences in numbers of regenerated plants between different treatment concentrations of NaN3(500 μmol/L or 100 μmol/L)and between different treatment times(5 h or 24 h)”

✓ located verbatim in the source · abstract, characters 786–980 · show in text ↑
Associationobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

NaN3 treatment times of 5 h or 24 h significantly differed in the number of regenerated plants.

Taxon
Brassica napusBrassica napuse0
Treatment
treatment times(5 h or 24 h)e5
Outcome
numbers of regenerated plantse5

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“on plant regeneration of cotyledons in two Brassica napus cultivars(Zheshuang 758 and Zheshuang 6) in vitro”

✓ located verbatim in the source · abstract, characters 108–215 · show in text ↑
e5

“There were significant differences in numbers of regenerated plants between different treatment concentrations of NaN3(500 μmol/L or 100 μmol/L)and between different treatment times(5 h or 24 h)”

✓ located verbatim in the source · abstract, characters 786–980 · show in text ↑
Associationobservedoriginal resultno effectmeasured

Claim · model-written summary

No obvious interactive effect was found among the mutagenic treatment factors on regeneration.

Taxon
Brassica napusBrassica napuse0
Treatment
different treatment factorse6
Outcome
interactive effecte6

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Brassica napus
Brassica napus · TaxonDidier Descouens · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“on plant regeneration of cotyledons in two Brassica napus cultivars(Zheshuang 758 and Zheshuang 6) in vitro”

✓ located verbatim in the source · abstract, characters 108–215 · show in text ↑
e6

“No obvious interactive effect was found among different treatment factors”

✓ located verbatim in the source · abstract, characters 981–1054 · show in text ↑

ethyl methanesulfonate · growth · Brassica napus (oilseed rape)Positive and negative results

4 papers: 1 positive, 3 negative or no effect

3 in the opposite direction

  1. negative
  2. negative
  3. negative
See all 4 papers →

uv · growth · Brassica napus (oilseed rape)Positive and negative results

10 papers: 4 positive, 6 negative or no effect

3 papers in the same direction as this paper · 6 in the opposite direction

  1. negative
  2. negative
  3. no effect

6 papers more in this group

See all 10 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.