Abstract
The major parameters controlling DNA delivery to wheat (Triticum aestivum. L) by particle bombardment were investigated, with four wheat cultivars from Southwest area of China as target tissues. The results indicated that when spermidine and CaCl 2,the precipitants for coating particles, were replaced with PEG4000 and Ca (NO 3) 2 respectively, the frequency of GUS transient expression was improved significantly. The bombardment parameters for immature spikes and immature embryos were tuned, and it was found that the transformation frequency was relatively high when immature spikes pre cultured in vitro for 1 day was used as target tissues with the bombardment distance in 9cm, and immature embryos for 14 days with the bombardment distance in 12cm. In addition, the dosage of gold powder and DNA per bombardment was one of the key factors for the successful transformation. In this study, using 14 days pre cultured immature embryos as target tissues, 125μg gold powder coated by 0.5μg DNA was the best combination.
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Claims 5
Claim · model-written summary
Replacing coating precipitants significantly improved transient GUS expression.
- Taxon
- wheat
Triticum aestivumt0 - Treatment
- PEG4000 and Ca (NO 3) 2e0
- Outcome
- frequency of GUS transient expressione0
- Direction
- improvede0
- Comparator
- spermidine and CaCl 2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when spermidine and CaCl 2,the precipitants for coating particles, were replaced with PEG4000 and Ca (NO 3) 2 respectively, the frequency of GUS transient expression was improved significantly.”
t0“wheat”
Claim · model-written summary
One-day precultured immature spikes at 9 cm had relatively high transformation frequency.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- immature spikese0
- Outcome
- transformation frequencye0
- Direction
- relatively highe0
- Value
- 9cme0
- Qualifier
- pre cultured in vitro for 1 daye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transformation frequency was relatively high when immature spikes pre cultured in vitro for 1 day was used as target tissues with the bombardment distance in 9cm”
t0“wheat”
Claim · model-written summary
Fourteen-day immature embryos at 12 cm had relatively high transformation frequency.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- immature embryose0
- Outcome
- transformation frequencye0
- Direction
- relatively highe0
- Value
- 12cme0
- Qualifier
- for 14 dayse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transformation frequency was relatively high when immature spikes pre cultured in vitro for 1 day was used as target tissues with the bombardment distance in 9cm, and immature embryos for 14 days with the bombardment distance in 12cm”
t0“wheat”
Claim · model-written summary
Gold powder and DNA dose was a key transformation factor.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- dosage of gold powder and DNA per bombardmente0
- Outcome
- successful transformatione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the dosage of gold powder and DNA per bombardment was one of the key factors for the successful transformation.”
t0“wheat”
Claim · model-written summary
For 14-day precultured immature embryos, 125μg gold with 0.5μg DNA was the best combination.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- immature embryose0
- Treatment
- 125μg gold powder coated by 0.5μg DNAe0
- Outcome
- best combinatione0
- Direction
- beste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“using 14 days pre cultured immature embryos as target tissues, 125μg gold powder coated by 0.5μg DNA was the best combination.”
t0“wheat”
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