Paper · 2004 · Journal of Peanut Science

Young Leaf Tissue Cultures and Efficient Plant Regeneration in Peanut

LI Mei-qin

4
claims
9
verbatim quotes
1
citations
2
research areas
—
Not open access

Abstract

Somatic embryogenesis and plant regeneration in young leaf tissue cultures of peanut (Arachis hypogaea L.) cultivar BaiShaGuo were studied. The young leaves were cut into 1 mm×1 mm, and cultured on MS medium supplemented with 1~3 mg/L 2,4-D and 1~5 mg/L BAP. High frequency (89% to 96%) embryogenic clusters derived from leaf explant were obtained on all media used. When the embryogenic clusters were being transferred onto MS medium supplemented with 10 mg/L BAP, or supplemented with 3 mg/LBAP and 1 mg/L ABA, 57% to 100% of calli with embryogenic cluster formed somatic embryos. The emberyos germinated and developed into shoots after being transferred onto MS medium supplemented with BAP (3, 4, 10 mg/L) and ABA (0, 1 mg/L). The highest frenquency of shoot regeneration was up to 96%.

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Claims 4

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

All tested media produced embryogenic clusters from peanut leaf explants at 89% to 96%.

Organ / stage
leaf explante0
Treatment
MS medium supplemented with 1~3 mg/L 2,4-D and 1~5 mg/L BAPe1
Outcome
embryogenic clusterse0
Direction
High frequencye0
Value
89% to 96%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“High frequency (89% to 96%) embryogenic clusters derived from leaf explant were obtained on all media used.”

✓ located verbatim in the source · abstract, characters 259–366 · show in text ↑
e1

“cultured on MS medium supplemented with 1~3 mg/L 2,4-D and 1~5 mg/L BAP”

✓ located verbatim in the source · abstract, characters 186–257 · show in text ↑
t0

“Peanut”

✓ located verbatim in the source · title, characters 63–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Transfer to either specified MS medium induced somatic embryos in 57% to 100% of calli with embryogenic clusters.

Treatment
MS medium supplemented with 10 mg/L BAP, or supplemented with 3 mg/LBAP and 1 mg/L ABAe0
Outcome
somatic embryose0
Direction
formede0
Value
57% to 100%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“When the embryogenic clusters were being transferred onto MS medium supplemented with 10 mg/L BAP, or supplemented with 3 mg/LBAP and 1 mg/L ABA, 57% to 100% of calli with embryogenic cluster formed somatic embryos.”

✓ located verbatim in the source · abstract, characters 367–582 · show in text ↑
t0

“Peanut”

✓ located verbatim in the source · title, characters 63–69 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Embryos germinated and developed into shoots after transfer to MS medium with BAP and ABA.

Treatment
MS medium supplemented with BAP (3, 4, 10 mg/L) and ABA (0, 1 mg/L)e0
Outcome
germinated and developed into shootse0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“The emberyos germinated and developed into shoots after being transferred onto MS medium supplemented with BAP (3, 4, 10 mg/L) and ABA (0, 1 mg/L).”

✓ located verbatim in the source · abstract, characters 583–730 · show in text ↑
t0

“Peanut”

✓ located verbatim in the source · title, characters 63–69 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

The highest shoot regeneration frequency reached 96%.

Outcome
frenquency of shoot regeneratione0
Direction
higheste0
Value
96%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Arachis hypogaea
Arachis hypogaea · Taxon葉子 · CC0 · iNaturalist ↗

Verbatim

e0

“The highest frenquency of shoot regeneration was up to 96%.”

✓ located verbatim in the source · abstract, characters 731–790 · show in text ↑
t0

“Peanut”

✓ located verbatim in the source · title, characters 63–69 · show in text ↑

(+)-abscisic acid · growth · Arachis hypogaea (peanut)Results differ

8 papers: 4 positive, 4 negative or no effect

3 papers in the same direction as this paper · 4 in the opposite direction

  1. negative
  2. negative
  3. negative

4 papers more in this group

See all 8 papers →

2,4-D · molecular: metabolites · Arachis hypogaea (peanut)Positive and negative results

2 papers: 1 positive, 1 negative or no effect

1 reporting no difference from an alternative

  1. no effect
See all 2 papers →

N-benzyladenine · growth · Arachis hypogaea (peanut)Positive and negative results

18 papers: 16 positive, 2 negative or no effect

15 papers in the same direction as this paper · 2 in the opposite direction

  1. positive
  2. negative
  3. negative

14 papers more in this group

See all 18 papers →

N-benzyladenine · molecular: metabolites · Arachis hypogaea (peanut)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

in vitro · growth · Arachis hypogaea (peanut)Positive and negative results

44 papers: 41 positive, 3 negative or no effect

40 papers in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. no effect
  3. no effect

40 papers more in this group

See all 44 papers →

in vitro · molecular: metabolites · Arachis hypogaea (peanut)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.