Abstract
Through pharmacological combined with laser scanning confocal microscope (LSCM) and spectrophotography to study the role of Ca2+ and NO in signaling during Vicia faba L. stomatal movement response to ethylene (Eth). The results showed that treatment with ethephon (0.004%, 0.04%, 0.4%) resulted in a time- and dose-dependent stomatal closure under light. NO scavenger cPTIO, nitrate reductase inhibitor NaN3, or extracellular Ca2+ chelation EGTA reduced ethylene-induced stomatal closure. Moreover, ethylene was shown to enhance nitric oxide levels and, corresponding, nitrate reductase activity. Inhibition of the nitrate reductase diminished ethylene-induced NO production in both stomatal guard cell and leaf. Finally, ethylene-induced NO levels and nitrate reductase activity decreased when Ca2+ was compromised. On the basis of biochemical and pharmacological experimental results, we can conclude that Ca2+ and NO were involved in the signal transduction pathway of ethylene induced stomatal closure. Nitrate reductase-derived NO may represents a novel downstream component of Ca2+ signaling cascade during ethylene-induced stomatal movement in Vicia faba L.
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Claims 10
Claim · model-written summary
Ethephon caused time- and dose-dependent stomatal closure under light.
- Taxon
- Vicia faba L
Vicia fabat0 - Treatment
- ethephon (0.004%, 0.04%, 0.4%)e0
- Outcome
- stomatal closuree0
- Direction
- closuree0
- Qualifier
- time- and dose-dependente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“treatment with ethephon (0.004%, 0.04%, 0.4%) resulted in a time- and dose-dependent stomatal closure under light”
t0“Vicia faba L”
Claim · model-written summary
The NO scavenger cPTIO reduced ethylene-induced stomatal closure.
- Taxon
- Vicia faba LVicia fabat0
- Treatment
- NO scavenger cPTIOe0
- Outcome
- ethylene-induced stomatal closuree0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO scavenger cPTIO, nitrate reductase inhibitor NaN3, or extracellular Ca2+ chelation EGTA reduced ethylene-induced stomatal closure”
t0“Vicia faba L”
Claim · model-written summary
The nitrate reductase inhibitor NaN3 reduced ethylene-induced stomatal closure.
- Taxon
- Vicia faba LVicia fabat0
- Treatment
- nitrate reductase inhibitor NaN3e0
- Outcome
- ethylene-induced stomatal closuree0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO scavenger cPTIO, nitrate reductase inhibitor NaN3, or extracellular Ca2+ chelation EGTA reduced ethylene-induced stomatal closure”
t0“Vicia faba L”
Claim · model-written summary
Extracellular Ca2+ chelation with EGTA reduced ethylene-induced stomatal closure.
- Taxon
- Vicia faba LVicia fabat0
- Treatment
- extracellular Ca2+ chelation EGTAe0
- Outcome
- ethylene-induced stomatal closuree0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO scavenger cPTIO, nitrate reductase inhibitor NaN3, or extracellular Ca2+ chelation EGTA reduced ethylene-induced stomatal closure”
t0“Vicia faba L”
Claim · model-written summary
Ethylene enhanced nitric oxide levels.
- Taxon
- Vicia faba LVicia fabat0
- Treatment
- ethylenee0
- Outcome
- nitric oxide levelse0
- Direction
- enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene was shown to enhance nitric oxide levels and, corresponding, nitrate reductase activity”
t0“Vicia faba L”
Claim · model-written summary
Ethylene enhanced nitrate reductase activity.
- Taxon
- Vicia faba LVicia fabat0
- Treatment
- ethylenee0
- Gene
- nitrate reductasee0
- Outcome
- nitrate reductase activitye0
- Direction
- enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene was shown to enhance nitric oxide levels and, corresponding, nitrate reductase activity”
t0“Vicia faba L”
Claim · model-written summary
Nitrate reductase inhibition diminished ethylene-induced NO production in guard cells and leaves.
- Taxon
- Vicia faba LVicia fabat0
- Organ / stage
- stomatal guard cell and leafe0
- Treatment
- Inhibition of the nitrate reductasee0
- Outcome
- ethylene-induced NO productione0
- Direction
- diminishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inhibition of the nitrate reductase diminished ethylene-induced NO production in both stomatal guard cell and leaf”
t0“Vicia faba L”
Claim · model-written summary
Compromising calcium reduced ethylene-induced NO levels.
- Taxon
- Vicia faba LVicia fabat0
- Treatment
- Ca2+ was compromisede0
- Outcome
- ethylene-induced NO levelse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene-induced NO levels and nitrate reductase activity decreased when Ca2+ was compromised”
t0“Vicia faba L”
Claim · model-written summary
Compromising calcium reduced nitrate reductase activity.
- Taxon
- Vicia faba LVicia fabat0
- Treatment
- Ca2+ was compromisede0
- Gene
- nitrate reductasee0
- Outcome
- nitrate reductase activitye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ethylene-induced NO levels and nitrate reductase activity decreased when Ca2+ was compromised”
t0“Vicia faba L”
Claim · model-written summary
Nitrate reductase-derived NO may act downstream of calcium in ethylene-induced stomatal movement.
- Taxon
- Vicia faba L.Vicia fabae0
- Gene
- Nitrate reductasee0
- Outcome
- ethylene-induced stomatal movemente0
- Qualifier
- downstream component of Ca2+ signaling cascadee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nitrate reductase-derived NO may represents a novel downstream component of Ca2+ signaling cascade during ethylene-induced stomatal movement in Vicia faba L.”
The same questions in other papers
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nitric oxide · physiology: gas exchange · Vicia faba (faba bean)Positive and negative results
3 papers in the same direction as this paper · 5 in the opposite direction
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5 papers more in this group
See all 9 papers →ethene · molecular: metabolites · Vicia faba (faba bean)Results agree
1 paper in the same direction as this paper
- positive
nitrogen · physiology: gas exchange · Vicia faba (faba bean)Results agree
1 paper in the same direction as this paper
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.