Abstract
In order to establish a suitable system for fiber growth and development during cotton ovule culture in vitro,effects of medium types,hormones and hormone combinations,ovule age etc.were studied,using CCRI 49 as material.As well,based on the established experiment system for ovule culture in vitro,the genotype effect in ovule culture was studied.The results showed that BT basic medium is the best medium for cotton ovule culture in vitro.Liquid culture was better than solid one although both of them could induce the fiber growth and development during the ovule culture.All the ovules sampled before flowering and after flowering can be used as explants in ovule culture,but the best one for fiber growth and development was the ovules sampled at flowering day.Hormone was necessary for fiber growth and development during ovule culture in vitro,all the hormones used in the experiment such as GA3,IAA,NAA,Ethylene,and BR could induce the fiber growth and development,and GA3was best one.The effects of hormone combinations on fiber growth during the ovule culture in vitro were better than that with single hormone individually,and 5.0 μmol.L-1IAA+5.0 μmol.L-1GA3was the best one among the hormone combinations used in the experiment.The fiber growth and development during the cotton ovule culture in vitro was different significantly among the genotypes used in the experiment,and the best hormone or hormone combination might be different for the different genotypes.
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Claims 15
Claim · model-written summary
BT basic medium was the best medium for cotton ovule culture.
- Taxon
- cotton
Gossypium hirsutume0 - Organ / stage
- ovulee0
- Treatment
- BT basic mediume0
- Outcome
- best mediume0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BT basic medium is the best medium for cotton ovule culture in vitro.”
Claim · model-written summary
Liquid culture outperformed solid culture for fiber growth and development.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- Liquid culturee0
- Outcome
- fiber growth and developmente0
- Direction
- bettere0
- Comparator
- solid onee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Liquid culture was better than solid one although both of them could induce the fiber growth and development during the ovule culture.”
t0“Cotton”
Claim · model-written summary
Solid and liquid cultures both induced fiber growth and development.
- Taxon
- CottonGossypium hirsutumt0
- Organ / stage
- ovulee0
- Outcome
- fiber growth and developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both of them could induce the fiber growth and development during the ovule culture.”
t0“Cotton”
Claim · model-written summary
Ovules sampled before and after flowering could be used as explants.
- Taxon
- CottonGossypium hirsutumt0
- Organ / stage
- ovulese0
- Outcome
- used as explantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All the ovules sampled before flowering and after flowering can be used as explants in ovule culture”
t0“Cotton”
Claim · model-written summary
Ovules sampled on flowering day were best for fiber growth and development.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- ovules sampled at flowering daye0
- Outcome
- fiber growth and developmente0
- Direction
- beste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the best one for fiber growth and development was the ovules sampled at flowering day.”
t0“Cotton”
Claim · model-written summary
Hormone was necessary for fiber growth and development in ovule culture.
- Taxon
- CottonGossypium hirsutumt0
- Outcome
- fiber growth and developmente0
- Qualifier
- Hormone was necessarye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Hormone was necessary for fiber growth and development during ovule culture in vitro”
t0“Cotton”
Claim · model-written summary
GA3 induced fiber growth and development and was the best hormone.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- GA3e0
- Outcome
- fiber growth and developmente0
- Direction
- best onee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all the hormones used in the experiment such as GA3,IAA,NAA,Ethylene,and BR could induce the fiber growth and development,and GA3was best one.”
t0“Cotton”
Claim · model-written summary
IAA induced fiber growth and development.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- IAAe0
- Outcome
- fiber growth and developmente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all the hormones used in the experiment such as GA3,IAA,NAA,Ethylene,and BR could induce the fiber growth and development”
t0“Cotton”
Claim · model-written summary
NAA induced fiber growth and development.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- NAAe0
- Outcome
- fiber growth and developmente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all the hormones used in the experiment such as GA3,IAA,NAA,Ethylene,and BR could induce the fiber growth and development”
t0“Cotton”
Claim · model-written summary
Ethylene induced fiber growth and development.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Ethylenee0
- Outcome
- fiber growth and developmente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all the hormones used in the experiment such as GA3,IAA,NAA,Ethylene,and BR could induce the fiber growth and development”
t0“Cotton”
Claim · model-written summary
BR induced fiber growth and development.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- BRe0
- Outcome
- fiber growth and developmente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all the hormones used in the experiment such as GA3,IAA,NAA,Ethylene,and BR could induce the fiber growth and development”
t0“Cotton”
Claim · model-written summary
Hormone combinations gave better fiber growth than single hormones.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- hormone combinationse0
- Outcome
- fiber growthe0
- Direction
- bettere0
- Comparator
- single hormonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The effects of hormone combinations on fiber growth during the ovule culture in vitro were better than that with single hormone individually”
t0“Cotton”
Claim · model-written summary
The 5.0 μmol.L-1IAA plus 5.0 μmol.L-1GA3 combination was the best tested combination.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- 5.0 μmol.L-1IAA+5.0 μmol.L-1GA3e0
- Outcome
- best onee0
- Direction
- beste0
- Comparator
- hormone combinationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“5.0 μmol.L-1IAA+5.0 μmol.L-1GA3was the best one among the hormone combinations used in the experiment.”
t0“Cotton”
Claim · model-written summary
Fiber growth and development differed significantly among genotypes.
- Taxon
- cottonGossypium hirsutume0
- Material
- genotypese0
- Outcome
- fiber growth and developmente0
- Direction
- differente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fiber growth and development during the cotton ovule culture in vitro was different significantly among the genotypes used in the experiment”
Claim · model-written summary
The best hormone or combination might differ among genotypes.
- Taxon
- CottonGossypium hirsutumt0
- Material
- genotypese0
- Outcome
- best hormone or hormone combinatione0
- Direction
- differente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the best hormone or hormone combination might be different for the different genotypes.”
t0“Cotton”
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