Abstract
Our current understanding of the role of phytohormones in the development of cotton fibers is derived largely from an amenable culture system in which cotton ovules, collected on the day of anthesis, are floated on liquid media. Under these conditions, supplemental auxin and gibberellin were found to promote fiber initiation and elongation. More recently, addition of low concentrations of the brassinosteroid brassinolide (BL) were also found to promote fiber elongation while a brassinosteroid biosynthesis inhibitor brassinazole2001 (Brz) inhibited fiber development. In order to elucidate the role of brassinosteroid in cotton fiber development further, we have performed a more detailed analysis of the effects of these chemicals on cultured cotton ovules. Our results confirm that exogenous BL promotes fiber elongation while treatment with Brz inhibits it. Furthermore, treatment of cotton floral buds with Brz results in the complete absence of fiber differentiation, indicating that BR is required for fiber initiation as well as elongation. Expression of fiber genes associated with cell elongation increased in ovules treated with BL and was suppressed by Brz treatment, establishing a correlation between brassinosteroid-regulated gene expression and fiber elongation. These results establish a clear connection between brassinosteroid and fiber development and open the door for genetic analysis of cotton development through direct modification of the brassinosteroid signal transduction pathway.
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Claims 7
Claim · model-written summary
Exogenous brassinolide (BL) promoted fiber elongation in cultured cotton ovules.
- Taxon
- Cotton
Gossypium hirsutumt0 - Treatment
- exogenous BLe1
- Outcome
- fiber elongatione1
- Direction
- promotese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassinosteroid Regulates Fiber Development on Cultured Cotton Ovules”
e1“Our results confirm that exogenous BL promotes fiber elongation while treatment with Brz inhibits it”
Claim · model-written summary
Brassinazole (Brz) treatment inhibited fiber elongation.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- treatment with Brze1
- Outcome
- fiber elongatione1
- Direction
- inhibitse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassinosteroid Regulates Fiber Development on Cultured Cotton Ovules”
e1“Our results confirm that exogenous BL promotes fiber elongation while treatment with Brz inhibits it”
Claim · model-written summary
Brz treatment of cotton floral buds completely eliminated fiber differentiation.
- Taxon
- CottonGossypium hirsutumt0
- Organ / stage
- floral budse2
- Treatment
- treatment of cotton floral buds with Brze2
- Outcome
- fiber differentiatione2
- Direction
- complete absencee2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassinosteroid Regulates Fiber Development on Cultured Cotton Ovules”
e2“treatment of cotton floral buds with Brz results in the complete absence of fiber differentiation”
Claim · model-written summary
Brassinosteroid is required for fiber initiation as well as elongation.
- Taxon
- CottonGossypium hirsutumt0
- Compound
- BRe3
- Outcome
- fiber initiatione3
- Qualifier
- required for fiber initiation as well as elongatione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassinosteroid Regulates Fiber Development on Cultured Cotton Ovules”
e3“BR is required for fiber initiation as well as elongation”
Claim · model-written summary
Exogenous BL increased expression of fiber genes associated with cell elongation in ovules.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- ovules treated with BLe4
- Gene
- fiber genese4
- Outcome
- Expression of fiber genes associated with cell elongatione4
- Direction
- increasede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassinosteroid Regulates Fiber Development on Cultured Cotton Ovules”
e4“Expression of fiber genes associated with cell elongation increased in ovules treated with BL and was suppressed by Brz treatment”
Claim · model-written summary
Brz treatment suppressed expression of fiber genes associated with cell elongation.
- Taxon
- CottonGossypium hirsutumt0
- Treatment
- Brz treatmente4
- Gene
- fiber genese4
- Outcome
- Expression of fiber genes associated with cell elongatione4
- Direction
- suppressede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassinosteroid Regulates Fiber Development on Cultured Cotton Ovules”
e4“Expression of fiber genes associated with cell elongation increased in ovules treated with BL and was suppressed by Brz treatment”
Claim · model-written summary
A correlation was established between brassinosteroid-regulated gene expression and fiber elongation.
- Taxon
- CottonGossypium hirsutumt0
- Gene
- brassinosteroid-regulated gene expressione5
- Compound
- brassinosteroide5
- Outcome
- fiber elongatione5
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Brassinosteroid Regulates Fiber Development on Cultured Cotton Ovules”
e5“establishing a correlation between brassinosteroid-regulated gene expression and fiber elongation”
The same questions in other papers
brassinolide · molecular: expression · Gossypium hirsutum (cotton)Positive and negative results
4 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
2 papers more in this group
See all 6 papers →brassinolide · quality: grain · Gossypium hirsutum (cotton)Results agree
6 papers in the same direction as this paper
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.