Paper · 2012 · He'nan nongye daxue xuebao

Cloning and expression analysis of a CBL-interacting protein kinase of maize

Tai Fu-ju

10
claims
20
verbatim quotes
0
citations
1
research areas
—
Not open access

Abstract

A ZmCIPK3 gene encoding putative CIPK was isolated from zea mays by the sequence homology.The deduced protein contains an N-terminal kinase domain and a C-terminal regulatory NAF domain,which is the signature sequence of plant CIPKs.Sequence analysis indicated that the open reading frame of the ZmCIPK3 gene consists of a 1 323bp encoding a polypeptide of 440 amino acids.Expression of the ZmCIPK3 was detected highly inducible by diverse signals such as drought,cold,NaCl and ABA.Further study showed that calcium increased the ZmCIPK3 expression level.In addition,we also found that a Ca2+ channel blockers(LaCl3) and a calcium chelator(EGTA) partially abolished(blocked) stimulus-induced expressions.These findings suggest that the ZmCIPK3 may be a stress and /or ABA-inducible gene in maize and the second messenger calcium mediates its expression responses to stress.

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Claims 10

Observationobservedoriginal resultmeasured

Claim · model-written summary

The maize ZmCIPK3 gene was isolated.

Taxon
zea maysZea mayse0
Gene
ZmCIPK3e0
Outcome
was isolatede0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“A ZmCIPK3 gene encoding putative CIPK was isolated from zea mays by the sequence homology”

✓ located verbatim in the source · abstract, characters 0–89 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

ZmCIPK3 protein contains kinase and NAF domains.

Taxon
maizeZea mayst0
Gene
ZmCIPK3e1
Outcome
N-terminal kinase domain and a C-terminal regulatory NAF domaine0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The deduced protein contains an N-terminal kinase domain and a C-terminal regulatory NAF domain”

✓ located verbatim in the source · abstract, characters 90–185 · show in text ↑
e1

“A ZmCIPK3 gene encoding putative CIPK was isolated from zea mays by the sequence homology”

✓ located verbatim in the source · abstract, characters 0–89 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 71–76 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

ZmCIPK3 has a 1 323bp open reading frame encoding 440 amino acids.

Taxon
maizeZea mayst0
Gene
ZmCIPK3e0
Outcome
open reading framee0
Value
1 323bpe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“open reading frame of the ZmCIPK3 gene consists of a 1 323bp encoding a polypeptide of 440 amino acids”

✓ located verbatim in the source · abstract, characters 270–372 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 71–76 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Drought induced ZmCIPK3 expression.

Taxon
maizeZea mayst0
Treatment
droughte0
Gene
ZmCIPK3e0
Outcome
Expression of the ZmCIPK3e0
Direction
induciblee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Expression of the ZmCIPK3 was detected highly inducible by diverse signals such as drought,cold,NaCl and ABA”

✓ located verbatim in the source · abstract, characters 373–481 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 71–76 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Cold induced ZmCIPK3 expression.

Taxon
maizeZea mayst0
Treatment
colde0
Gene
ZmCIPK3e0
Outcome
Expression of the ZmCIPK3e0
Direction
induciblee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Expression of the ZmCIPK3 was detected highly inducible by diverse signals such as drought,cold,NaCl and ABA”

✓ located verbatim in the source · abstract, characters 373–481 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 71–76 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

NaCl induced ZmCIPK3 expression.

Taxon
maizeZea mayst0
Treatment
NaCle0
Gene
ZmCIPK3e0
Outcome
Expression of the ZmCIPK3e0
Direction
induciblee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Expression of the ZmCIPK3 was detected highly inducible by diverse signals such as drought,cold,NaCl and ABA”

✓ located verbatim in the source · abstract, characters 373–481 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 71–76 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

ABA induced ZmCIPK3 expression.

Taxon
maizeZea mayst0
Treatment
ABAe0
Gene
ZmCIPK3e0
Outcome
Expression of the ZmCIPK3e0
Direction
induciblee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Expression of the ZmCIPK3 was detected highly inducible by diverse signals such as drought,cold,NaCl and ABA”

✓ located verbatim in the source · abstract, characters 373–481 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 71–76 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Calcium increased ZmCIPK3 expression.

Taxon
maizeZea mayst0
Treatment
calciume0
Gene
ZmCIPK3e0
Outcome
ZmCIPK3 expression levele0
Direction
increasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“calcium increased the ZmCIPK3 expression level”

✓ located verbatim in the source · abstract, characters 508–554 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 71–76 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

LaCl3 partly blocked stimulus induced ZmCIPK3 expression.

Taxon
maizeZea mayst0
Treatment
LaCl3e0
Outcome
stimulus-induced expressionse0
Direction
abolishede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“a Ca2+ channel blockers(LaCl3) and a calcium chelator(EGTA) partially abolished(blocked) stimulus-induced expressions”

✓ located verbatim in the source · abstract, characters 586–703 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 71–76 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

EGTA partly blocked stimulus induced ZmCIPK3 expression.

Taxon
maizeZea mayst0
Treatment
EGTAe0
Outcome
stimulus-induced expressionse0
Direction
abolishede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“a Ca2+ channel blockers(LaCl3) and a calcium chelator(EGTA) partially abolished(blocked) stimulus-induced expressions”

✓ located verbatim in the source · abstract, characters 586–703 · show in text ↑
t0

“maize”

✓ located verbatim in the source · title, characters 71–76 · show in text ↑

(+)-abscisic acid · molecular: expression · Zea mays (maize)Results differ

51 papers: 37 positive, 14 negative or no effect

36 papers in the same direction as this paper · 14 in the opposite direction

  1. negative
  2. no effect
  3. no effect

47 papers more in this group

See all 51 papers →

sodium chloride · molecular: expression · Zea mays (maize)Positive and negative results

10 papers: 7 positive, 3 negative or no effect

6 papers in the same direction as this paper · 2 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. negative
  3. no effect

6 papers more in this group

See all 10 papers →

calcium atom · molecular: expression · Zea mays (maize)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

cold · molecular: expression · Zea mays (maize)Positive and negative results

32 papers: 21 positive, 11 negative or no effect

20 papers in the same direction as this paper · 10 in the opposite direction · 1 reporting no difference from an alternative

  1. negative
  2. no effect
  3. no effect

28 papers more in this group

See all 32 papers →

drought · molecular: expression · Zea mays (maize)Positive and negative results

65 papers: 43 positive, 22 negative or no effect

42 papers in the same direction as this paper · 22 in the opposite direction

  1. no effect
  2. negative
  3. no effect

61 papers more in this group

See all 65 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.