Abstract
A ZmCIPK3 gene encoding putative CIPK was isolated from zea mays by the sequence homology.The deduced protein contains an N-terminal kinase domain and a C-terminal regulatory NAF domain,which is the signature sequence of plant CIPKs.Sequence analysis indicated that the open reading frame of the ZmCIPK3 gene consists of a 1 323bp encoding a polypeptide of 440 amino acids.Expression of the ZmCIPK3 was detected highly inducible by diverse signals such as drought,cold,NaCl and ABA.Further study showed that calcium increased the ZmCIPK3 expression level.In addition,we also found that a Ca2+ channel blockers(LaCl3) and a calcium chelator(EGTA) partially abolished(blocked) stimulus-induced expressions.These findings suggest that the ZmCIPK3 may be a stress and /or ABA-inducible gene in maize and the second messenger calcium mediates its expression responses to stress.
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Claims 10
Claim · model-written summary
The maize ZmCIPK3 gene was isolated.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“A ZmCIPK3 gene encoding putative CIPK was isolated from zea mays by the sequence homology”
Claim · model-written summary
ZmCIPK3 protein contains kinase and NAF domains.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“The deduced protein contains an N-terminal kinase domain and a C-terminal regulatory NAF domain”
e1“A ZmCIPK3 gene encoding putative CIPK was isolated from zea mays by the sequence homology”
t0“maize”
Claim · model-written summary
ZmCIPK3 has a 1 323bp open reading frame encoding 440 amino acids.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“open reading frame of the ZmCIPK3 gene consists of a 1 323bp encoding a polypeptide of 440 amino acids”
t0“maize”
Claim · model-written summary
Drought induced ZmCIPK3 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Expression of the ZmCIPK3 was detected highly inducible by diverse signals such as drought,cold,NaCl and ABA”
t0“maize”
Claim · model-written summary
Cold induced ZmCIPK3 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Expression of the ZmCIPK3 was detected highly inducible by diverse signals such as drought,cold,NaCl and ABA”
t0“maize”
Claim · model-written summary
NaCl induced ZmCIPK3 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Expression of the ZmCIPK3 was detected highly inducible by diverse signals such as drought,cold,NaCl and ABA”
t0“maize”
Claim · model-written summary
ABA induced ZmCIPK3 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“Expression of the ZmCIPK3 was detected highly inducible by diverse signals such as drought,cold,NaCl and ABA”
t0“maize”
Claim · model-written summary
Calcium increased ZmCIPK3 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“calcium increased the ZmCIPK3 expression level”
t0“maize”
Claim · model-written summary
LaCl3 partly blocked stimulus induced ZmCIPK3 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“a Ca2+ channel blockers(LaCl3) and a calcium chelator(EGTA) partially abolished(blocked) stimulus-induced expressions”
t0“maize”
Claim · model-written summary
EGTA partly blocked stimulus induced ZmCIPK3 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“a Ca2+ channel blockers(LaCl3) and a calcium chelator(EGTA) partially abolished(blocked) stimulus-induced expressions”
t0“maize”
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