Abstract
Calcium dependent protein phosphorylation was studied in corn (Zea mays L.) root tips. Prior to in vivo protein phosphorylation experiments, the effect of calcium, ethyleneglycol-bis-(beta-aminoethyl ether)-N-N' -tetraacetic acid (EGTA) and calcium ionophore (A-23187) on phosphorus uptake was studied. Calcium increased phosphorus uptake, whereas EGTA and A-23187 decreased it. Consequently, phosphorus concentration in the media was adjusted so as to attain similar uptake in different treatments. Phosphoproteins were analyzed by two-dimensional gel electrophoresis. Distinct changes in phosphorylation were observed following altered calcium levels. Calcium depletion in root tips with EGTA and A-23187 decreased protein phosphorylation. However, replenishment of calcium following EGTA and ionophore pretreatment enhanced phosphorylation of proteins. Preloading of the root tips with 32P in the presence of EGTA and A-23187 followed by a ten minute calcium treatment, resulted in increased phosphorylation indicating the involvement of calcium, calcium and calmodulin-dependent kinases. Calmodulin antagonist W-7 was effective in inhibiting calcium-promoted phosphorylation. These studies suggest a physiological role for calcium-dependent phosphorylation in calcium-mediated processes in plants.
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Claims 10
Claim · model-written summary
Calcium increased phosphorus uptake in corn root tips.
- Taxon
- corn
Zea mays subsp. mayst0 - Organ / stage
- root tipst0
- Treatment
- Calciume0
- Outcome
- phosphorus uptakee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Calcium increased phosphorus uptake, whereas EGTA and A-23187 decreased it.”
t0“Calcium dependent protein phosphorylation was studied in corn (Zea mays L.) root tips.”
Claim · model-written summary
EGTA decreased phosphorus uptake in corn root tips.
- Taxon
- cornZea mays subsp. mayst0
- Organ / stage
- root tipst0
- Treatment
- EGTAe0
- Outcome
- phosphorus uptakee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Calcium increased phosphorus uptake, whereas EGTA and A-23187 decreased it.”
t0“Calcium dependent protein phosphorylation was studied in corn (Zea mays L.) root tips.”
Claim · model-written summary
A-23187 decreased phosphorus uptake in corn root tips.
- Taxon
- cornZea mays subsp. mayst0
- Organ / stage
- root tipst0
- Treatment
- A-23187e0
- Outcome
- phosphorus uptakee0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Calcium increased phosphorus uptake, whereas EGTA and A-23187 decreased it.”
t0“Calcium dependent protein phosphorylation was studied in corn (Zea mays L.) root tips.”
Claim · model-written summary
Altered calcium levels changed protein phosphorylation.
- Taxon
- cornZea mays subsp. mayst0
- Treatment
- altered calcium levelse0
- Outcome
- phosphorylatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Distinct changes in phosphorylation were observed following altered calcium levels.”
t0“Calcium dependent protein phosphorylation was studied in corn (Zea mays L.) root tips.”
Claim · model-written summary
EGTA calcium depletion decreased protein phosphorylation in root tips.
- Taxon
- cornZea mays subsp. mayst0
- Organ / stage
- root tipse0
- Treatment
- EGTAe0
- Outcome
- protein phosphorylatione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Calcium depletion in root tips with EGTA and A-23187 decreased protein phosphorylation.”
t0“Calcium dependent protein phosphorylation was studied in corn (Zea mays L.) root tips.”
Claim · model-written summary
A-23187 calcium depletion decreased protein phosphorylation in root tips.
- Taxon
- cornZea mays subsp. mayst0
- Organ / stage
- root tipse0
- Treatment
- A-23187e0
- Outcome
- protein phosphorylatione0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Calcium depletion in root tips with EGTA and A-23187 decreased protein phosphorylation.”
t0“Calcium dependent protein phosphorylation was studied in corn (Zea mays L.) root tips.”
Claim · model-written summary
Calcium replenishment after EGTA pretreatment enhanced protein phosphorylation.
- Taxon
- cornZea mays subsp. mayst0
- Treatment
- replenishment of calcium following EGTAe0
- Outcome
- phosphorylation of proteinse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, replenishment of calcium following EGTA and ionophore pretreatment enhanced phosphorylation of proteins.”
t0“Calcium dependent protein phosphorylation was studied in corn (Zea mays L.) root tips.”
Claim · model-written summary
Calcium replenishment after ionophore pretreatment enhanced protein phosphorylation.
- Taxon
- cornZea mays subsp. mayst0
- Treatment
- replenishment of calcium following EGTA and ionophore pretreatmente0
- Outcome
- phosphorylation of proteinse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“However, replenishment of calcium following EGTA and ionophore pretreatment enhanced phosphorylation of proteins.”
t0“Calcium dependent protein phosphorylation was studied in corn (Zea mays L.) root tips.”
Claim · model-written summary
Ten-minute calcium treatment after 32P preloading increased phosphorylation.
- Taxon
- cornZea mays subsp. mayst0
- Treatment
- ten minute calcium treatmente0
- Outcome
- phosphorylatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Preloading of the root tips with 32P in the presence of EGTA and A-23187 followed by a ten minute calcium treatment, resulted in increased phosphorylation indicating the involvement of calcium, calcium and calmodulin-dependent kinases.”
t0“Calcium dependent protein phosphorylation was studied in corn (Zea mays L.) root tips.”
Claim · model-written summary
W-7 inhibited calcium-promoted phosphorylation.
- Taxon
- cornZea mays subsp. mayst0
- Treatment
- W-7e0
- Outcome
- calcium-promoted phosphorylatione0
- Direction
- inhibitinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Calmodulin antagonist W-7 was effective in inhibiting calcium-promoted phosphorylation.”
t0“Calcium dependent protein phosphorylation was studied in corn (Zea mays L.) root tips.”
The same questions in other papers
calcium atom · nutrient: content · Zea mays (maize)Positive and negative results
20 papers in the same direction as this paper · 5 in the opposite direction
- no effect
- negative
- no effect
22 papers more in this group
See all 26 papers →calcium atom · molecular: expression · Zea mays (maize)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.