Abstract
Shoot multiplication of the Alfalfa cultivars "Elçi" and "Mesa-Sirsa" was achieved from the shoot tips of in vitro germinated seedlings. Shoot tips were cultured in a basal medium supplemented with different concentrations of 6-benzylaminopurine (BAP) and a-naphthaleneacetic acid (NAA). The number of shoots obtained was higher in the medium containing 2 mg/l BAP and 0.2 mg/l NAA for "Elçi" and 1 mg/l BAP and 0.2 mg/l NAA for "Mesa-Sirsa". In both cultivars, the mean length of shoots was significantly higher on the MS medium supplemented with 2 mg/l BAP and 0.2 mg/l NAA. The percentage of roots reached up to 80-90 % with increasing IBA (indole butyric acid) concentrations in the culture medium. The mean number of roots per shoot was also influenced by IBA concentrations, but there were no treatment effects on the root length of either cultivar. However, the mean root length was higher in "Elçi" than in "Mesa-Sirsa". No change was observed in the number of somatic chromosome of the shoots developed in the culture.
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Claims 8
Claim · model-written summary
Shoot number of alfalfa cultivar Elçi was higher in medium with 2 mg/l BAP and 0.2 mg/l NAA.
- Taxon
- Medicago sativa
Medicago sativat0 - Material
- Elçie0
- Treatment
- 2 mg/l BAP and 0.2 mg/l NAAe0
- Outcome
- number of shootse0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The number of shoots obtained was higher in the medium containing 2 mg/l BAP and 0.2 mg/l NAA for "Elçi" and 1 mg/l BAP and 0.2 mg/l NAA for "Mesa-Sirsa"”
t0“Medicago sativa”
Claim · model-written summary
Shoot number of alfalfa cultivar Mesa-Sirsa was higher in medium with 1 mg/l BAP and 0.2 mg/l NAA.
- Taxon
- Medicago sativaMedicago sativat0
- Material
- Mesa-Sirsae0
- Treatment
- 1 mg/l BAP and 0.2 mg/l NAAe0
- Outcome
- number of shootse0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The number of shoots obtained was higher in the medium containing 2 mg/l BAP and 0.2 mg/l NAA for "Elçi" and 1 mg/l BAP and 0.2 mg/l NAA for "Mesa-Sirsa"”
t0“Medicago sativa”
Claim · model-written summary
In both alfalfa cultivars mean shoot length was significantly higher on MS medium with 2 mg/l BAP and 0.2 mg/l NAA.
- Taxon
- Medicago sativaMedicago sativat0
- Treatment
- 2 mg/l BAP and 0.2 mg/l NAAe0
- Outcome
- mean length of shootse0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In both cultivars, the mean length of shoots was significantly higher on the MS medium supplemented with 2 mg/l BAP and 0.2 mg/l NAA”
t0“Medicago sativa”
Claim · model-written summary
The percentage of rooted shoots in alfalfa rose to as much as 80-90 % as IBA concentration in the culture medium increased.
- Taxon
- Medicago sativaMedicago sativat0
- Treatment
- increasing IBA (indole butyric acid) concentrationse0
- Outcome
- percentage of rootse0
- Direction
- increaseinferrede0
- Value
- 80-90 %e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The percentage of roots reached up to 80-90 % with increasing IBA (indole butyric acid) concentrations in the culture medium”
t0“Medicago sativa”
Claim · model-written summary
The mean number of roots per shoot in alfalfa was influenced by IBA concentration.
- Taxon
- Medicago sativaMedicago sativat0
- Treatment
- IBA concentrationse0
- Outcome
- mean number of roots per shoote0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mean number of roots per shoot was also influenced by IBA concentrations”
t0“Medicago sativa”
Claim · model-written summary
Treatments had no effect on root length in either alfalfa cultivar.
- Taxon
- Medicago sativaMedicago sativat0
- Treatment
- treatmente0
- Outcome
- root lengthe0
- Direction
- no_changeinferrede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there were no treatment effects on the root length of either cultivar”
t0“Medicago sativa”
Claim · model-written summary
Mean root length was higher in alfalfa cultivar Elçi than in Mesa-Sirsa.
- Taxon
- Medicago sativaMedicago sativat0
- Material
- Elçie0
- Outcome
- mean root lengthe0
- Direction
- highere0
- Comparator
- Mesa-Sirsae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mean root length was higher in "Elçi" than in "Mesa-Sirsa"”
t0“Medicago sativa”
Claim · model-written summary
No change in somatic chromosome number was observed in shoots developed in culture.
- Taxon
- Medicago sativaMedicago sativat0
- Outcome
- number of somatic chromosomee0
- Direction
- No changee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No change was observed in the number of somatic chromosome of the shoots developed in the culture”
t0“Medicago sativa”
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