Abstract
In search for the genes involved in low-temperature, high-salt and drought stress response in turf grass tall fescue (Festuca arundinacea Schreb.), a DREB-like transcription factor gene, FaDREB1A, was isolated from a cDNA library constructed from cold treated (4 ℃) F. arundinacea seedlings. The FaDREB1A had an open reading frame of 717 bp with a 3’-UTR of 143 bp. The putative protein FaDREB1A contained a highly conserved EREBP/AP2 domain. In yeast one-hybrid analysis FaDREB1A protein was specifically bound to DRE elements and activated the expression of the reporter genes of HIS3 and LacZ. Southern blotting results showed that the FaDREB1A gene was a single or few copies in the genome of F. arundinacea. Northern blotting results indicated that FaDREB1A gene was obviously induced by low-temperature, high-salt and drought stresses, but not by ABA, suggesting an important role of FaDREB1A in response to abiotic stresses in F. arundinacea. Fig 5, Ref 30
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Claims 10
Claim · model-written summary
A DREB-like transcription factor gene, FaDREB1A, was isolated from a cDNA library of cold-treated tall fescue seedlings.
- Taxon
- F. arundinacea
Lolium arundinaceume1 - Organ / stage
- seedlingse1
- Treatment
- cold treated (4 ℃)e1
- Gene
- FaDREB1Ae1
- Outcome
- a DREB-like transcription factor genee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tall fescue (Festuca arundinacea Schreb.)”
e1“a DREB-like transcription factor gene, FaDREB1A, was isolated from a cDNA library constructed from cold treated (4 ℃) F. arundinacea seedlings”
Claim · model-written summary
The FaDREB1A gene has an open reading frame of 717 bp.
- Taxon
- Festuca arundinaceaLolium arundinaceume0
- Gene
- FaDREB1Ae2
- Outcome
- open reading framee2
- Value
- 717 bpe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tall fescue (Festuca arundinacea Schreb.)”
e2“The FaDREB1A had an open reading frame of 717 bp with a 3’-UTR of 143 bp”
Claim · model-written summary
The FaDREB1A gene has a 3’-UTR of 143 bp.
- Taxon
- Festuca arundinaceaLolium arundinaceume0
- Gene
- FaDREB1Ae2
- Outcome
- 3’-UTRe2
- Value
- 143 bpe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tall fescue (Festuca arundinacea Schreb.)”
e2“The FaDREB1A had an open reading frame of 717 bp with a 3’-UTR of 143 bp”
Claim · model-written summary
The putative FaDREB1A protein contains a highly conserved EREBP/AP2 domain.
- Taxon
- Festuca arundinaceaLolium arundinaceume0
- Gene
- FaDREB1Ae3
- Outcome
- EREBP/AP2 domaine3
- Qualifier
- highly conservede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tall fescue (Festuca arundinacea Schreb.)”
e3“The putative protein FaDREB1A contained a highly conserved EREBP/AP2 domain”
Claim · model-written summary
In yeast one-hybrid analysis, FaDREB1A protein specifically bound to DRE elements.
- Taxon
- Festuca arundinaceaLolium arundinaceume0
- Gene
- FaDREB1Ae4
- Outcome
- bound to DRE elementse4
- Qualifier
- In yeast one-hybrid analysise4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tall fescue (Festuca arundinacea Schreb.)”
e4“In yeast one-hybrid analysis FaDREB1A protein was specifically bound to DRE elements and activated the expression of the reporter genes of HIS3 and LacZ”
Claim · model-written summary
In yeast one-hybrid analysis, FaDREB1A activated the expression of the HIS3 and LacZ reporter genes.
- Taxon
- Festuca arundinaceaLolium arundinaceume0
- Gene
- FaDREB1Ae4
- Outcome
- expression of the reporter genese4
- Direction
- activatede4
- Qualifier
- HIS3 and LacZe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tall fescue (Festuca arundinacea Schreb.)”
e4“In yeast one-hybrid analysis FaDREB1A protein was specifically bound to DRE elements and activated the expression of the reporter genes of HIS3 and LacZ”
Claim · model-written summary
FaDREB1A is present as a single or few copies in the genome of tall fescue.
- Taxon
- Festuca arundinaceaLolium arundinaceume0
- Gene
- FaDREB1Ae5
- Outcome
- copies in the genomee5
- Value
- single or few copiese5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tall fescue (Festuca arundinacea Schreb.)”
e5“Southern blotting results showed that the FaDREB1A gene was a single or few copies in the genome of F. arundinacea”
Claim · model-written summary
Low-temperature, high-salt and drought stresses induced FaDREB1A gene expression in tall fescue.
- Taxon
- Festuca arundinaceaLolium arundinaceume0
- Treatment
- low-temperature, high-salt and drought stressese6
- Gene
- FaDREB1Ae6
- Outcome
- FaDREB1A genee6
- Direction
- inducede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tall fescue (Festuca arundinacea Schreb.)”
e6“Northern blotting results indicated that FaDREB1A gene was obviously induced by low-temperature, high-salt and drought stresses, but not by ABA”
Claim · model-written summary
ABA did not induce FaDREB1A gene expression in tall fescue.
- Taxon
- Festuca arundinaceaLolium arundinaceume0
- Treatment
- ABAe6
- Gene
- FaDREB1Ae6
- Outcome
- FaDREB1A genee6
- Direction
- no_changeinferrede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“tall fescue (Festuca arundinacea Schreb.)”
e6“Northern blotting results indicated that FaDREB1A gene was obviously induced by low-temperature, high-salt and drought stresses, but not by ABA”
Claim · model-written summary
FaDREB1A plays an important role in the response of F. arundinacea to abiotic stresses.
- Taxon
- F. arundinaceaLolium arundinaceume7
- Gene
- FaDREB1Ae7
- Outcome
- response to abiotic stressese7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“suggesting an important role of FaDREB1A in response to abiotic stresses in F. arundinacea”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Lolium arundinaceum (tall fescue)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
drought · molecular: expression · Lolium arundinaceum (tall fescue)Positive and negative results
2 papers in the same direction as this paper · 4 in the opposite direction
- negative
- negative
- negative
3 papers more in this group
See all 7 papers →cold · molecular: expression · Lolium arundinaceum (tall fescue)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.