Abstract
Festuca arundinacea and F. pratensis are the models in forage grasses to recognize the molecular basis of drought, salt and frost tolerance, respectively. Transcription profiles of plasma membrane intrinsic proteins (PIPs) and tonoplast intrinsic proteins (TIPs) aquaporin genes were obtained for leaves of Festuca species treated with different abiotic stimuli. F. arundinacea plants were exposed to drought and salt stress, whereas F. pratensis plants were cold-hardened. Changes in genes expression measured with use of real time qRT-PCR method were compared between two genotypes characterized with a significantly different level of each stress tolerance. Under drought the transcript level of PIP1;2 and TIP1;1 aquaporin decreased in both analyzed F. arundinacea genotypes, whereas for PIP2;1 only in a high drought tolerant plant. A salt treatment caused a reduction of PIP1;2 transcript level in a high salt tolerant genotype and an increase of TIP1;1 transcript abundance in both F. arundinacea genotypes, but it did not influence the expression of PIP2;1 aquaporin. During cold-hardening a decrease of PIP1;2, PIP2;1, and TIP1;1 aquaporin transcripts was observed, both in high and low frost tolerant genotypes. The obtained results revealed that the selected genotypes responded in a different way to abiotic stresses application. A reduced level of PIP1;2 transcript in F. arundinacea low drought tolerant genotype corresponded with a faster water loss and a lowering of photosynthesis efficiency and gas exchange during drought conditions. In F. pratensis, cold acclimation was associated with a lower level of aquaporin transcripts in both high and low frost tolerant genotypes. This is the first report on aquaporin transcriptional profiling under abiotic stress condition in forage grasses.
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Claims 8
Claim · model-written summary
Drought decreased PIP1;2 and TIP1;1 transcripts in both F. arundinacea genotypes.
- Taxon
- F. arundinacea
Lolium arundinaceume0 - Material
- both analyzed F. arundinacea genotypese0
- Treatment
- droughte0
- Gene
- PIP1;2 and TIP1;1e0
- Outcome
- transcript level of PIP1;2 and TIP1;1 aquaporine0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought the transcript level of PIP1;2 and TIP1;1 aquaporin decreased in both analyzed F. arundinacea genotypes”
Claim · model-written summary
Drought decreased PIP2;1 transcripts only in the high drought tolerant F. arundinacea genotype.
- Taxon
- F. arundinaceaLolium arundinaceume0
- Material
- high drought tolerant plante0
- Treatment
- droughte0
- Gene
- PIP2;1e0
- Outcome
- PIP2;1e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought the transcript level of PIP1;2 and TIP1;1 aquaporin decreased in both analyzed F. arundinacea genotypes, whereas for PIP2;1 only in a high drought tolerant plant”
t0“Festuca species”
Claim · model-written summary
Salt treatment reduced PIP1;2 transcript level in the high salt tolerant genotype.
- Material
- high salt tolerant genotypee0
- Treatment
- salt treatmente0
- Gene
- PIP1;2e0
- Outcome
- PIP1;2 transcript levele0
- Direction
- reductione0
FestucaNot reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A salt treatment caused a reduction of PIP1;2 transcript level in a high salt tolerant genotype”
t0“Festuca species”
Claim · model-written summary
Salt treatment increased TIP1;1 transcript abundance in both F. arundinacea genotypes.
- Taxon
- F. arundinaceaLolium arundinaceume0
- Treatment
- salt treatmente0
- Gene
- TIP1;1e0
- Outcome
- TIP1;1 transcript abundancee0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A salt treatment caused a reduction of PIP1;2 transcript level in a high salt tolerant genotype and an increase of TIP1;1 transcript abundance in both F. arundinacea genotypes”
t0“Abiotic stresses influence”
Claim · model-written summary
Salt treatment did not affect PIP2;1 expression.
- Treatment
- salt treatmente0
- Gene
- PIP2;1e0
- Outcome
- expression of PIP2;1 aquaporine0
- Direction
- did not influencee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A salt treatment caused a reduction of PIP1;2 transcript level in a high salt tolerant genotype and an increase of TIP1;1 transcript abundance in both F. arundinacea genotypes, but it did not influence the expression of PIP2;1 aquaporin”
t0“Festuca species”
Claim · model-written summary
Cold hardening decreased PIP1;2, PIP2;1, and TIP1;1 transcripts in high and low frost tolerant F. pratensis genotypes.
- Taxon
- F. pratensis
Festuca pratensist0 - Treatment
- cold-hardeninge0
- Gene
- PIP1;2, PIP2;1, and TIP1;1e0
- Outcome
- PIP1;2, PIP2;1, and TIP1;1 aquaporin transcriptse0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During cold-hardening a decrease of PIP1;2, PIP2;1, and TIP1;1 aquaporin transcripts was observed, both in high and low frost tolerant genotypes”
t0“whereas F. pratensis plants were cold-hardened”
Claim · model-written summary
Reduced PIP1;2 transcript in low drought tolerant F. arundinacea corresponded with faster water loss.
- Taxon
- F. arundinaceaLolium arundinaceume0
- Material
- low drought tolerant genotypee0
- Treatment
- droughte0
- Gene
- PIP1;2e0
- Outcome
- water losse0
- Direction
- fastere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A reduced level of PIP1;2 transcript in F. arundinacea low drought tolerant genotype corresponded with a faster water loss and a lowering of photosynthesis efficiency and gas exchange during drought conditions”
t0“Abiotic stresses”
Claim · model-written summary
Reduced PIP1;2 transcript in low drought tolerant F. arundinacea corresponded with lower photosynthesis efficiency and gas exchange.
- Taxon
- F. arundinaceaLolium arundinaceume0
- Material
- low drought tolerant genotypee0
- Treatment
- drought conditionse0
- Gene
- PIP1;2e0
- Outcome
- photosynthesis efficiency and gas exchangee0
- Direction
- loweringe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A reduced level of PIP1;2 transcript in F. arundinacea low drought tolerant genotype corresponded with a faster water loss and a lowering of photosynthesis efficiency and gas exchange during drought conditions”
The same questions in other papers
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See all 7 papers →cold · molecular: expression · Festuca pratensis (meadow ryegrass)Results agree
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- no effect
salinity · molecular: expression · Lolium arundinaceum (tall fescue)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.