Abstract
DREB transcription factors are unique trans-acting factors that involved in low-temperature,high-salt and drought stress responses in plants.To isolate DREB genes from tall fescue(Festuca arundinacea Schreb),we constructed a cDNA library of cold(4℃) stressed F.arundinacea seedlings and identified a cDNA clone,FaDREB1,by screening the library with DREB1A gene of Arabidopsis as probe.The FaDREB1 has an open reading frame of 651bp with a 3'-UTR of 229bp.The putative protein contains a highly conserved EREBP/AP2 domain.In yeast one-hybrid analysis FaDREB1 protein was specifically bound to DRE elements and activated the expression of the reporter genes of HIS3 and LacZ.Northern blotting results indicated that FaDREB1 gene obviously induced by low-temperature,but not by high-salt,drought stress and ABA,suggesting an important role of FaDREB1 in response to cold stress in F.arundinacea.
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Claims 10
Claim · model-written summary
A FaDREB1 cDNA clone was identified from tall fescue.
- Taxon
- TALL FESCUE
Lolium arundinaceumt0 - Gene
- FaDREB1e0
- Outcome
- identified a cDNA clonee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“TALL FESCUE”
e0“identified a cDNA clone,FaDREB1,by screening the library”
Claim · model-written summary
FaDREB1 has a 651bp open reading frame.
- Taxon
- TALL FESCUELolium arundinaceumt0
- Gene
- FaDREB1e0
- Outcome
- open reading framee0
- Value
- 651bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“TALL FESCUE”
e0“The FaDREB1 has an open reading frame of 651bp with a 3'-UTR of 229bp”
Claim · model-written summary
FaDREB1 has a 229bp 3-prime untranslated region.
- Taxon
- TALL FESCUELolium arundinaceumt0
- Gene
- FaDREB1e0
- Outcome
- 3'-UTRe0
- Value
- 229bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“TALL FESCUE”
e0“The FaDREB1 has an open reading frame of 651bp with a 3'-UTR of 229bp”
Claim · model-written summary
The putative FaDREB1 protein contains a conserved EREBP/AP2 domain.
- Taxon
- TALL FESCUELolium arundinaceumt0
- Gene
- EREBP/AP2e0
- Outcome
- highly conserved EREBP/AP2 domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“TALL FESCUE”
e0“The putative protein contains a highly conserved EREBP/AP2 domain”
Claim · model-written summary
FaDREB1 protein bound DRE elements and activated HIS3 and LacZ reporter genes in yeast.
- Taxon
- TALL FESCUELolium arundinaceumt0
- Gene
- FaDREB1e0
- Outcome
- bound to DRE elements and activated the expression of the reporter genes of HIS3 and LacZe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“TALL FESCUE”
e0“FaDREB1 protein was specifically bound to DRE elements and activated the expression of the reporter genes of HIS3 and LacZ”
Claim · model-written summary
Low temperature induced FaDREB1 expression.
- Taxon
- TALL FESCUELolium arundinaceumt0
- Treatment
- low-temperaturee0
- Gene
- FaDREB1e0
- Outcome
- FaDREB1 genee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“TALL FESCUE”
e0“FaDREB1 gene obviously induced by low-temperature,but not by high-salt,drought stress and ABA”
Claim · model-written summary
High salt did not induce FaDREB1.
- Taxon
- TALL FESCUELolium arundinaceumt0
- Treatment
- high-salte0
- Gene
- FaDREB1e0
- Outcome
- FaDREB1 genee0
- Direction
- not bye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“TALL FESCUE”
e0“FaDREB1 gene obviously induced by low-temperature,but not by high-salt,drought stress and ABA”
Claim · model-written summary
Drought stress did not induce FaDREB1.
- Taxon
- TALL FESCUELolium arundinaceumt0
- Treatment
- drought stresse0
- Gene
- FaDREB1e0
- Outcome
- FaDREB1 genee0
- Direction
- not bye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“TALL FESCUE”
e0“FaDREB1 gene obviously induced by low-temperature,but not by high-salt,drought stress and ABA”
Claim · model-written summary
ABA did not induce FaDREB1.
- Taxon
- TALL FESCUELolium arundinaceumt0
- Treatment
- ABAe0
- Gene
- FaDREB1e0
- Outcome
- FaDREB1 genee0
- Direction
- not bye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“TALL FESCUE”
e0“FaDREB1 gene obviously induced by low-temperature,but not by high-salt,drought stress and ABA”
Claim · model-written summary
The authors suggest an important role for FaDREB1 in cold stress response.
- Taxon
- F.arundinaceaLolium arundinaceume0
- Gene
- FaDREB1e0
- Outcome
- response to cold stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting an important role of FaDREB1 in response to cold stress in F.arundinacea”
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3 papers in the same direction as this paper · 3 in the opposite direction
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3 papers more in this group
See all 7 papers →cold · molecular: expression · Lolium arundinaceum (tall fescue)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.