Paper · 2013 · The Journal of Horticultural Science and Biotechnology

Effects of Persian walnut leaf extracts on seed germination, seedling growth, and some physiological characteristics of the basil (Ocimum basilicumL.) cultivar ‘Genovese’

Monad Dadi; Davood Bakhshi; Gholamali Peyvast; et al.

11
claims
20
verbatim quotes
1
citations
3
research areas
—
Not open access

Abstract

SummaryThis study was conducted to elucidate the allelopathic effects of a series of dilutions of Persian walnut (Juglans regia) leaf extracts [i.e., undiluted, 1:2 (v/v), 1:4 (v/v), or 1:8 (v/v) dilutions] and distilled water (control) on the characteristics of seed germination, seedling growth, and subsequent physiological changes in the basil (Ocimum basilicum L.) cultivar, ‘Genovese’. The results indicated that all aqueous leaf extracts of walnut had statistically significant inhibitory effects on the germination of basil seeds and on seedling growth characteristics. Seed germination percentages, the lengths and fresh weights (FWs) of the plumule and the radicle, the FWs and dry weights (DWs) of the shoots and roots, and leaf areas decreased significantly with increasing concentrations of aqueous walnut leaf extract. The DWs of the plumules and radicles were increased by the undiluted walnut leaf extract. Moreover, it was found that the yields of essential oils and total phenolics, and the anti-oxidant activities of basil seedlings increased with increasing concentrations of aqueous walnut leaf extract, whereas the relative water contents of basil leaves, leaf water potentials, as well as the total chlorophyll and carotenoid contents of basil leaves decreased significantly. The highest protein contents and peroxidase (POD) activities were achieved using undiluted or 1:2 (v/v) diluted aqueous walnut leaf extracts, respectively. The results of this study suggest that cultivation of the basil cultivar, ‘Genovese’ may be appropriate for walnut tree-based intercropping systems, based on the increases in anti-oxidant capacity and the accumulation of secondary metabolites.

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Claims 11

Intervention effectobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Aqueous walnut leaf extracts significantly inhibited germination of basil seeds and seedling growth compared with distilled water.

Material
‘Genovese’e0
Organ / stage
seedse1
Treatment
aqueous leaf extracts of walnute1
Outcome
germination of basil seeds and on seedling growth characteristicse1
Direction
inhibitory effectse1
Comparator
distilled water (control)e2

Not reportedSetting

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the basil (Ocimum basilicum L.) cultivar, ‘Genovese’”

✓ located verbatim in the source · abstract, characters 338–390 · show in text ↑
e1

“all aqueous leaf extracts of walnut had statistically significant inhibitory effects on the germination of basil seeds and on seedling growth characteristics”

✓ located verbatim in the source · abstract, characters 419–576 · show in text ↑
e2

“distilled water (control) on the characteristics of seed germination”

✓ located verbatim in the source · abstract, characters 211–279 · show in text ↑
Associationobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Seed germination percentage decreased significantly as walnut leaf extract concentration increased.

Treatment
increasing concentrations of aqueous walnut leaf extracte3
Outcome
Seed germination percentagese3
Direction
decreasede3
Qualifier
significantlye3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the basil (Ocimum basilicum L.) cultivar, ‘Genovese’”

✓ located verbatim in the source · abstract, characters 338–390 · show in text ↑
e3

“Seed germination percentages, the lengths and fresh weights (FWs) of the plumule and the radicle, the FWs and dry weights (DWs) of the shoots and roots, and leaf areas decreased significantly with increasing concentrations of aqueous walnut leaf extract”

✓ located verbatim in the source · abstract, characters 578–831 · show in text ↑
Associationobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Lengths and fresh weights of the plumule and radicle decreased significantly as walnut leaf extract concentration increased.

Treatment
increasing concentrations of aqueous walnut leaf extracte3
Outcome
the lengths and fresh weights (FWs) of the plumule and the radiclee3
Direction
decreasede3
Qualifier
significantlye3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the basil (Ocimum basilicum L.) cultivar, ‘Genovese’”

✓ located verbatim in the source · abstract, characters 338–390 · show in text ↑
e3

“Seed germination percentages, the lengths and fresh weights (FWs) of the plumule and the radicle, the FWs and dry weights (DWs) of the shoots and roots, and leaf areas decreased significantly with increasing concentrations of aqueous walnut leaf extract”

✓ located verbatim in the source · abstract, characters 578–831 · show in text ↑
Associationobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Shoot and root fresh and dry weights and leaf area decreased significantly as walnut leaf extract concentration increased.

Treatment
increasing concentrations of aqueous walnut leaf extracte3
Outcome
the FWs and dry weights (DWs) of the shoots and roots, and leaf arease3
Direction
decreasede3
Qualifier
significantlye3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the basil (Ocimum basilicum L.) cultivar, ‘Genovese’”

✓ located verbatim in the source · abstract, characters 338–390 · show in text ↑
e3

“Seed germination percentages, the lengths and fresh weights (FWs) of the plumule and the radicle, the FWs and dry weights (DWs) of the shoots and roots, and leaf areas decreased significantly with increasing concentrations of aqueous walnut leaf extract”

✓ located verbatim in the source · abstract, characters 578–831 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Undiluted walnut leaf extract increased the dry weights of basil plumules and radicles.

Organ / stage
plumules and radiclese4
Treatment
undiluted walnut leaf extracte4
Outcome
The DWs of the plumules and radiclese4
Direction
increasede4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the basil (Ocimum basilicum L.) cultivar, ‘Genovese’”

✓ located verbatim in the source · abstract, characters 338–390 · show in text ↑
e4

“The DWs of the plumules and radicles were increased by the undiluted walnut leaf extract”

✓ located verbatim in the source · abstract, characters 833–921 · show in text ↑
Associationobservedoriginal resultpositivemeasured

Claim · model-written summary

Essential oil yield, total phenolics and antioxidant activity of basil seedlings increased with increasing walnut leaf extract concentration.

Organ / stage
seedlingse5
Treatment
increasing concentrations of aqueous walnut leaf extracte5
Outcome
yields of essential oils and total phenolics, and the anti-oxidant activitiese5
Direction
increasede5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the basil (Ocimum basilicum L.) cultivar, ‘Genovese’”

✓ located verbatim in the source · abstract, characters 338–390 · show in text ↑
e5

“the yields of essential oils and total phenolics, and the anti-oxidant activities of basil seedlings increased with increasing concentrations of aqueous walnut leaf extract”

✓ located verbatim in the source · abstract, characters 951–1123 · show in text ↑
Associationobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Relative water content and leaf water potential of basil leaves decreased significantly.

Organ / stage
leavese6
Outcome
relative water contents of basil leaves, leaf water potentialse6
Direction
decreasede6
Qualifier
significantlye6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e6

“the relative water contents of basil leaves, leaf water potentials, as well as the total chlorophyll and carotenoid contents of basil leaves decreased significantly”

✓ located verbatim in the source · abstract, characters 1133–1297 · show in text ↑
Associationobservedoriginal resultnegativesignificant (as stated)measured

Claim · model-written summary

Total chlorophyll and carotenoid contents of basil leaves decreased significantly.

Organ / stage
leavese6
Outcome
total chlorophyll and carotenoid contentse6
Direction
decreasede6
Qualifier
significantlye6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e6

“the relative water contents of basil leaves, leaf water potentials, as well as the total chlorophyll and carotenoid contents of basil leaves decreased significantly”

✓ located verbatim in the source · abstract, characters 1133–1297 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

Undiluted or 1:2 diluted walnut leaf extract gave the highest basil protein contents.

Treatment
undiluted or 1:2 (v/v) diluted aqueous walnut leaf extractse7
Outcome
protein contentse7
Direction
higheste7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the basil (Ocimum basilicum L.) cultivar, ‘Genovese’”

✓ located verbatim in the source · abstract, characters 338–390 · show in text ↑
e7

“The highest protein contents and peroxidase (POD) activities were achieved using undiluted or 1:2 (v/v) diluted aqueous walnut leaf extracts, respectively”

✓ located verbatim in the source · abstract, characters 1299–1453 · show in text ↑
Associationobservedoriginal resultmeasured

Claim · model-written summary

The 1:2 diluted walnut leaf extract gave the highest basil peroxidase (POD) activity.

Treatment
1:2 (v/v) diluted aqueous walnut leaf extractse7
Outcome
peroxidase (POD) activitiese7
Direction
higheste7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the basil (Ocimum basilicum L.) cultivar, ‘Genovese’”

✓ located verbatim in the source · abstract, characters 338–390 · show in text ↑
e7

“The highest protein contents and peroxidase (POD) activities were achieved using undiluted or 1:2 (v/v) diluted aqueous walnut leaf extracts, respectively”

✓ located verbatim in the source · abstract, characters 1299–1453 · show in text ↑
Recommendationauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

Basil cultivar Genovese may be appropriate for walnut tree-based intercropping systems.

Material
‘Genovese’e7
Outcome
walnut tree-based intercropping systemse7

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Ocimum basilicum
Ocimum basilicum · TaxonCastielli · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e7

“The results of this study suggest that cultivation of the basil cultivar, ‘Genovese’ may be appropriate for walnut tree-based intercropping systems, based on the increases in anti-oxidant capacity and the accumulation of secondary metabolites”

✓ located verbatim in the source · abstract, characters 1455–1697 · show in text ↑

botanical · growth · Ocimum basilicum (basil)Positive and negative results

19 papers: 13 positive, 6 negative or no effect

5 papers in the same direction as this paper · 13 in the opposite direction

  1. positive
  2. positive
  3. positive

15 papers more in this group

See all 19 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.