Abstract
Plant cell suspension cultures are widely used for the production of recombinant proteins and secondary metabolites. One of the most important steps during process development is the optimization of yields by testing different cultivation parameters, including the components of the growth medium. However, we have shown that the biomass yield of a cell suspension culture derived from the pear cultivar Pyrus communis cv. Champagner Bratbirne can be significantly improved solely by varying the temperature, inoculum density, illumination, and incubation time. In contrast to medium optimization, these simple physical factors are easily controlled and varied, thereby reducing the effort required. Using an experimental design approach, we improved the biomass yield from 146 g fresh weight (FW)/L to 407 g FW/L in only 5 weeks, simultaneously reducing the costs of goods sold per kg biomass from € 125 to € 45. Our simple approach therefore offers a rapid, efficient and economical process for the optimization of plant cell suspension cultures.
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Claims 4
Claim · model-written summary
Varying temperature, inoculum density, illumination and incubation time significantly improved biomass yield of a pear cell suspension culture.
- Taxon
- Pyrus communis
_Poirier_Fleurs_et_feuilles.jpg?width=60)
Pyrus communise0 - Material
- Champagner Bratbirnee0
- Organ / stage
- cell suspension culturee0
- Treatment
- varying the temperature, inoculum density, illumination, and incubation timee0
- Outcome
- biomass yielde0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Poirier_Fleurs_et_feuilles.jpg?width=500)
Verbatim
e0“we have shown that the biomass yield of a cell suspension culture derived from the pear cultivar Pyrus communis cv. Champagner Bratbirne can be significantly improved solely by varying the temperature, inoculum density, illumination, and incubation time”
Claim · model-written summary
The experimental design raised pear cell culture biomass yield to 407 g FW/L in 5 weeks.
- Taxon
- Pyrus communisPyrus communise0
- Material
- Champagner Bratbirnee0
- Treatment
- experimental design approache1
- Outcome
- biomass yielde1
- Direction
- improvede1
- Value
- 407 g FW/Le1
- Qualifier
- from 146 g fresh weight (FW)/Le1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we have shown that the biomass yield of a cell suspension culture derived from the pear cultivar Pyrus communis cv. Champagner Bratbirne can be significantly improved solely by varying the temperature, inoculum density, illumination, and incubation time”
e1“Using an experimental design approach, we improved the biomass yield from 146 g fresh weight (FW)/L to 407 g FW/L in only 5 weeks, simultaneously reducing the costs of goods sold per kg biomass from € 125 to € 45”
Claim · model-written summary
The experimental design reduced costs of goods sold per kg pear cell culture biomass from 125 to 45 euro.
- Taxon
- Pyrus communisPyrus communise0
- Material
- Champagner Bratbirnee0
- Treatment
- experimental design approache1
- Outcome
- costs of goods sold per kg biomasse1
- Direction
- reducinge1
- Value
- € 125 to € 45e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we have shown that the biomass yield of a cell suspension culture derived from the pear cultivar Pyrus communis cv. Champagner Bratbirne can be significantly improved solely by varying the temperature, inoculum density, illumination, and incubation time”
e1“Using an experimental design approach, we improved the biomass yield from 146 g fresh weight (FW)/L to 407 g FW/L in only 5 weeks, simultaneously reducing the costs of goods sold per kg biomass from € 125 to € 45”
Claim · model-written summary
The approach offers a rapid, efficient and economical process for optimizing plant cell suspension cultures.
- Taxon
- Pyrus communisPyrus communise0
- Outcome
- optimization of plant cell suspension culturese2
- Qualifier
- rapid, efficient and economicale2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we have shown that the biomass yield of a cell suspension culture derived from the pear cultivar Pyrus communis cv. Champagner Bratbirne can be significantly improved solely by varying the temperature, inoculum density, illumination, and incubation time”
e2“Our simple approach therefore offers a rapid, efficient and economical process for the optimization of plant cell suspension cultures”
The same questions in other papers
inoculation · yield · Pyrus communis (pear)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- negative
- positive
light · yield · Pyrus communis (pear)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.