Abstract
In this study,full length cDNA sequence of a putative Δ1-pyrroline-5-carboxylate synthase gene(LpP5CS)was cloned from perennial ryegrass(Lolium perenne L.‘Derby')leaves using RT-PCR and rapid amplification of cDNA ends(RACE).Sequence analysis showed that the nucleotide sequence of this gene is 2 528 bp,containing a complete open reading frame and encoding 716 amino acids.Nucleotide and amino acid sequence analysis revealed that LpP5CS shares high identity with the orthologs from Triticum aestivum(92.05%,93.99%)and Oryza sativa(85.82%,87.99%),respectively.Semi-quantitative RT-PCR analysis showed that LpP5CS expressed in different tissues.Various elevated levels of LpP5CS expression have been detected when the seedling roots exposed to high salinity.Tested with salt solution, its expression was higher than control,and the highest in leaves,the lowest in roots,The contents of LpP5CS increased first then decreased with the processing time is extended after 200 mmol · L-1 NaCl treatment.Finally,subcellular localzation assays showed that the LpP5CS protein was present in the nucleus and at the plasmalemma.
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Claims 10
Claim · model-written summary
LpP5CS was cloned from perennial ryegrass leaves.
- Taxon
- perennial ryegrass
Lolium perennee0 - Organ / stage
- leavese0
- Gene
- LpP5CSe0
- Outcome
- full length cDNA sequencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“full length cDNA sequence of a putative Δ1-pyrroline-5-carboxylate synthase gene(LpP5CS)was cloned from perennial ryegrass(Lolium perenne L.‘Derby')leaves”
Claim · model-written summary
The LpP5CS sequence is 2 528 bp and encodes 716 amino acids.
- Taxon
- perennial ryegrassLolium perennet0
- Gene
- LpP5CSe1
- Outcome
- nucleotide sequencee0
- Value
- 2 528 bpe0
- Qualifier
- encoding 716 amino acidse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the nucleotide sequence of this gene is 2 528 bp,containing a complete open reading frame and encoding 716 amino acids”
t0“perennial ryegrass (Lolium perenne L.)”
e1“gene(LpP5CS)was cloned”
Claim · model-written summary
LpP5CS had high nucleotide and amino acid identity with Triticum aestivum orthologs.
- Taxon
- perennial ryegrassLolium perennet0
- Gene
- LpP5CSe0
- Outcome
- identitye0
- Value
- 92.05%,93.99%e0
- Comparator
- Triticum aestivume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LpP5CS shares high identity with the orthologs from Triticum aestivum(92.05%,93.99%)”
t0“perennial ryegrass (Lolium perenne L.)”
Claim · model-written summary
LpP5CS had high nucleotide and amino acid identity with Oryza sativa orthologs.
- Taxon
- perennial ryegrassLolium perennet0
- Gene
- LpP5CSe0
- Outcome
- identitye0
- Value
- 85.82%,87.99%e0
- Comparator
- Oryza sativae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LpP5CS shares high identity with the orthologs from Triticum aestivum(92.05%,93.99%)and Oryza sativa(85.82%,87.99%)”
t0“perennial ryegrass (Lolium perenne L.)”
Claim · model-written summary
LpP5CS was expressed in different tissues.
- Taxon
- perennial ryegrassLolium perennet0
- Gene
- LpP5CSe0
- Outcome
- expressed in different tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“LpP5CS expressed in different tissues”
t0“perennial ryegrass (Lolium perenne L.)”
Claim · model-written summary
High salinity elevated LpP5CS expression in seedling roots.
- Taxon
- perennial ryegrassLolium perennet0
- Organ / stage
- seedling rootse0
- Treatment
- high salinitye0
- Gene
- LpP5CSe0
- Outcome
- LpP5CS expressione0
- Direction
- elevatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Various elevated levels of LpP5CS expression have been detected when the seedling roots exposed to high salinity”
t0“perennial ryegrass (Lolium perenne L.)”
Claim · model-written summary
LpP5CS expression was higher under salt solution than in controls.
- Taxon
- perennial ryegrassLolium perennet0
- Treatment
- salt solutione0
- Gene
- LpP5CSe1
- Outcome
- expressione0
- Direction
- highere0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tested with salt solution, its expression was higher than control”
t0“perennial ryegrass (Lolium perenne L.)”
e1“gene(LpP5CS)was cloned”
Claim · model-written summary
LpP5CS expression was highest in leaves and lowest in roots under salt solution.
- Taxon
- perennial ryegrassLolium perennet0
- Organ / stage
- leavese0
- Gene
- LpP5CSe1
- Outcome
- expressione0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its expression was higher than control,and the highest in leaves,the lowest in roots”
t0“perennial ryegrass (Lolium perenne L.)”
e1“gene(LpP5CS)was cloned”
Claim · model-written summary
LpP5CS content rose and then fell over time after NaCl treatment.
- Taxon
- perennial ryegrassLolium perennet0
- Treatment
- 200 mmol · L-1 NaCl treatmente0
- Gene
- LpP5CSe0
- Outcome
- contents of LpP5CSe0
- Direction
- increased first then decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The contents of LpP5CS increased first then decreased with the processing time is extended after 200 mmol · L-1 NaCl treatment”
t0“perennial ryegrass (Lolium perenne L.)”
Claim · model-written summary
LpP5CS protein was localized in the nucleus and plasmalemma.
- Taxon
- perennial ryegrassLolium perennet0
- Gene
- LpP5CS proteine0
- Outcome
- present in the nucleus and at the plasmalemmae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the LpP5CS protein was present in the nucleus and at the plasmalemma”
t0“perennial ryegrass (Lolium perenne L.)”
The same questions in other papers
salinity · molecular: expression · Lolium perenne (perennial ryegrass)Positive and negative results
3 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.