Abstract
Conversion of nongrain biomass into liquid fuel is a sustainable approach to energy demands as global population increases. Previously, we showed that iron can act as a catalyst to enhance the degradation of lignocellulosic biomass for biofuel production. However, direct addition of iron catalysts to biomass pretreatment is diffusion-limited, would increase the cost and complexity of biorefinery unit operations and may have deleterious environmental impacts. Here, we show a new strategy for in planta accumulation of iron throughout the volume of the cell wall where iron acts as a catalyst in the deconstruction of lignocellulosic biomass. We engineered CBM-IBP fusion polypeptides composed of a carbohydrate-binding module family 11 (CBM11) and an iron-binding peptide (IBP) for secretion into Arabidopsis and rice cell walls. CBM-IBP transformed Arabidopsis and rice plants show significant increases in iron accumulation and biomass conversion compared to respective controls. Further, CBM-IBP rice shows a 35% increase in seed iron concentration and a 40% increase in seed yield in greenhouse experiments. CBM-IBP rice potentially could be used to address iron deficiency, the most common and widespread nutritional disorder according to the World Health Organization.
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Claims 6
Claim · model-written summary
Transformed Arabidopsis had significantly increased iron accumulation.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- CBM-IBP transformede0
- Outcome
- iron accumulatione0
- Direction
- increasese0
- Comparator
- respective controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CBM-IBP transformed Arabidopsis and rice plants show significant increases in iron accumulation and biomass conversion compared to respective controls”
Claim · model-written summary
Transformed rice had significantly increased iron accumulation.
- Taxon
- rice
Oryza sativae0 - Treatment
- CBM-IBP transformede0
- Outcome
- iron accumulatione0
- Direction
- increasese0
- Comparator
- respective controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CBM-IBP transformed Arabidopsis and rice plants show significant increases in iron accumulation and biomass conversion compared to respective controls”
Claim · model-written summary
Transformed Arabidopsis had significantly increased biomass conversion.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- CBM-IBP transformede0
- Outcome
- biomass conversione0
- Direction
- increasese0
- Comparator
- respective controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CBM-IBP transformed Arabidopsis and rice plants show significant increases in iron accumulation and biomass conversion compared to respective controls”
Claim · model-written summary
Transformed rice had significantly increased biomass conversion.
- Taxon
- riceOryza sativae0
- Treatment
- CBM-IBP transformede0
- Outcome
- biomass conversione0
- Direction
- increasese0
- Comparator
- respective controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CBM-IBP transformed Arabidopsis and rice plants show significant increases in iron accumulation and biomass conversion compared to respective controls”
Claim · model-written summary
Transformed rice had 35% higher seed iron concentration.
- Taxon
- riceOryza sativae0
- Treatment
- CBM-IBPe0
- Outcome
- seed iron concentratione0
- Direction
- increasee0
- Value
- 35%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CBM-IBP rice shows a 35% increase in seed iron concentration”
Claim · model-written summary
Transformed rice had 40% higher seed yield in greenhouse experiments.
- Taxon
- riceOryza sativae0
- Treatment
- CBM-IBPe0
- Outcome
- seed yielde0
- Direction
- increasee0
- Value
- 40%e0
- Setting
- greenhouse experimentse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CBM-IBP rice shows a 35% increase in seed iron concentration and a 40% increase in seed yield in greenhouse experiments”
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