Abstract
SUMMARY: The Arabidopsis thaliana NAC domain transcription factor, vascular-related NAC-DOMAIN7 (VND7), plays a pivotal role in regulating the differentiation of root protoxylem vessels. In order to understand the mechanisms underscoring the function of VND7 in vessel differentiation in more detail, we conducted extensive molecular analyses in yeast (Saccharomyces cerevisiae), Arabidopsis, and Nicotiana tabacum L. cv. Bright Yellow 2 (tobacco BY-2) cells. The transcriptional activation activity of VND7 was confirmed in yeast and Arabidopsis, and the C-terminal region was shown to be required for VND7 transcriptional activation. Expression of the C-terminus-truncated VND7 protein under the control of the native VND7 promoter resulted in inhibition of the normal development of metaxylem vessels in roots and vessels in aerial organs, as well as protoxylem vessels in roots. The expression pattern of VND7 overlapped that of VND2 to VND5 in most of the differentiating vessels. Furthermore, a yeast two-hybrid assay revealed the ability of VND7 to form homodimers and heterodimers with other VND proteins via their N-termini, which include the NAC domain. The heterologous expression of VND7 in tobacco BY-2 cells demonstrated that the stability of VND7 could be regulated by proteasome-mediated degradation. Together these data suggest that VND7 regulates the differentiation of all types of vessels in roots and shoots, possibly in cooperation with VND2 to VND5 and other regulatory proteins.
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Claims 8
Claim · model-written summary
VND7 transcriptional activation was confirmed in Arabidopsis and yeast.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- VND7e0
- Outcome
- transcriptional activation activity of VND7 was confirmede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcriptional activation activity of VND7 was confirmed in yeast and Arabidopsis, and the C-terminal region was shown to be required for VND7 transcriptional activation.”
Claim · model-written summary
The C-terminal region was required for VND7 transcriptional activation.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- VND7e0
- Outcome
- VND7 transcriptional activatione0
- Qualifier
- C-terminal region was shown to be requirede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The transcriptional activation activity of VND7 was confirmed in yeast and Arabidopsis, and the C-terminal region was shown to be required for VND7 transcriptional activation.”
Claim · model-written summary
Truncated VND7 inhibited development of root metaxylem, root protoxylem and aerial vessels.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Expression of the C-terminus-truncated VND7 proteine0
- Gene
- VND7e0
- Outcome
- normal development of metaxylem vessels in roots and vessels in aerial organs, as well as protoxylem vessels in rootse0
- Direction
- inhibitione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of the C-terminus-truncated VND7 protein under the control of the native VND7 promoter resulted in inhibition of the normal development of metaxylem vessels in roots and vessels in aerial organs, as well as protoxylem vessels in roots.”
t0“Arabidopsis”
Claim · model-written summary
VND7 expression overlapped VND2 to VND5 in differentiating vessels.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- VND7e0
- Outcome
- expression pattern of VND7 overlapped that of VND2 to VND5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression pattern of VND7 overlapped that of VND2 to VND5 in most of the differentiating vessels.”
t0“Arabidopsis”
Claim · model-written summary
VND7 formed homodimers through its N terminus.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- VND7e0
- Outcome
- form homodimerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, a yeast two-hybrid assay revealed the ability of VND7 to form homodimers and heterodimers with other VND proteins via their N-termini, which include the NAC domain.”
t0“Arabidopsis”
Claim · model-written summary
VND7 formed heterodimers with other VND proteins through their N termini.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- VND7e0
- Outcome
- heterodimers with other VND proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, a yeast two-hybrid assay revealed the ability of VND7 to form homodimers and heterodimers with other VND proteins via their N-termini, which include the NAC domain.”
t0“Arabidopsis”
Claim · model-written summary
In tobacco BY-2 cells, VND7 stability could be regulated by proteasome-mediated degradation.
- Taxon
- tobacco
Nicotiana tabacume0 - Material
- BY-2e0
- Gene
- VND7e0
- Outcome
- stability of VND7e0
- Qualifier
- proteasome-mediated degradatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The heterologous expression of VND7 in tobacco BY-2 cells demonstrated that the stability of VND7 could be regulated by proteasome-mediated degradation.”
Claim · model-written summary
VND7 may regulate all vessel types in roots and shoots with VND2 to VND5.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- VND7e0
- Outcome
- differentiation of all types of vessels in roots and shootse0
- Qualifier
- possibly in cooperation with VND2 to VND5 and other regulatory proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Together these data suggest that VND7 regulates the differentiation of all types of vessels in roots and shoots, possibly in cooperation with VND2 to VND5 and other regulatory proteins.”
t0“Arabidopsis”
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