Abstract
Pollen plants from anther culture in rice breeding allows selection of fine strains in early generations. Exploitation of anther culture technique in research is limited due to very low regeneration frequency of indica cultivars and the transfer of anther culture traits from japonica to indica varieties is important in improving these rice varieties. In this study the optimization of media requirements and culture conditions for high frequency callus induction and plant regeneration of several indica × japonica F1 hybrids were evaluated using improved anther culture media. Five indica rice varieties ( BG 90-2, BG 379-2, BG 94-1, Dahanala and Suduru samba) and two japonica varieties (Hu Lo Tao and Chuan 4) were selected and F1 hybrids were taken with all possible crosses between them. Panicles were cold pre treated at 5 o C for 7 days and anthers were cultured in agar solidified modified N6, B5 and Millers media. Calli were transferred to MS medium for plant regeneration. Best callus induction frequencies (0.8 – 29.4%) were obtained in N6 medium containing 5% (w/v) sucrose, than B5 (0.5 – 19.2%) and Millers (0.2 – 19.8%) media. The F1 hybrids were more responsive to anther culture than their parents. Highest callus induction frequency of 29.4% could observe in N6 medium for F1 hybrid Hu Lo Tao × BG 90-2. The green plant regeneration frequency of calli induced on N6 medium was higher than the other two media and the highest frequency of 41.0% was occurred in F1 hybrids of Hu Lo Tao × BG 90-2. Green plants were transferred to pots with 50 – 75% survival rate. Out of 99 plants survived, 28 plants had more than 5% spikelet fertility. Modified N6 medium had positive effect on anther culture performance and the F1 hybrid Hu Low Tao × Bg 90-2 had the best performance.
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Claims 9
Claim · model-written summary
N6 medium gave higher callus induction frequencies (0.8 to 29.4%) than the B5 and Millers media.
- Taxon
- indica rice
Oryza sativa Indica Groupt0 - Treatment
- N6 medium containing 5% (w/v) sucrosee1
- Outcome
- callus induction frequenciese1
- Direction
- higherinferrede1
- Value
- 0.8 – 29.4%e1
- Qualifier
- Millers (0.2 – 19.8%) mediae1
- Comparator
- B5e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Indica Rice Varieties”
e1“Best callus induction frequencies (0.8 – 29.4%) were obtained in N6 medium containing 5% (w/v) sucrose, than B5 (0.5 – 19.2%) and Millers (0.2 – 19.8%) media”
Claim · model-written summary
F1 hybrids responded more strongly to anther culture than their parents.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- F1 hybridse2
- Outcome
- responsive to anther culturee2
- Comparator
- their parentse2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Indica Rice Varieties”
e2“The F1 hybrids were more responsive to anther culture than their parents”
Claim · model-written summary
The highest callus induction frequency (29.4%) was on N6 medium for the F1 hybrid Hu Lo Tao × BG 90-2.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- F1 hybrid Hu Lo Tao × BG 90-2e3
- Treatment
- N6 mediume3
- Outcome
- callus induction frequencye3
- Value
- 29.4%e3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Indica Rice Varieties”
e3“Highest callus induction frequency of 29.4% could observe in N6 medium for F1 hybrid Hu Lo Tao × BG 90-2”
Claim · model-written summary
Green plant regeneration frequency of calli was higher on N6 medium than on the other two media.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- N6 mediume4
- Outcome
- green plant regeneration frequencye4
- Direction
- highere4
- Comparator
- other two mediae4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Indica Rice Varieties”
e4“The green plant regeneration frequency of calli induced on N6 medium was higher than the other two media”
Claim · model-written summary
The highest green plant regeneration frequency (41.0%) occurred in F1 hybrids of Hu Lo Tao × BG 90-2.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- F1 hybrids of Hu Lo Tao × BG 90-2e5
- Outcome
- green plant regeneration frequencye4
- Value
- 41.0%e5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Indica Rice Varieties”
e4“The green plant regeneration frequency of calli induced on N6 medium was higher than the other two media”
e5“the highest frequency of 41.0% was occurred in F1 hybrids of Hu Lo Tao × BG 90-2”
Claim · model-written summary
Green plants transferred to pots had a 50 to 75% survival rate.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Outcome
- survival ratee6
- Value
- 50 – 75%e6
- Setting
- potse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Indica Rice Varieties”
e6“Green plants were transferred to pots with 50 – 75% survival rate”
Claim · model-written summary
28 of the 99 surviving plants had more than 5% spikelet fertility.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Outcome
- spikelet fertilitye7
- Value
- 28 plantse7
- Qualifier
- Out of 99 plants survivede7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Indica Rice Varieties”
e7“Out of 99 plants survived, 28 plants had more than 5% spikelet fertility”
Claim · model-written summary
Modified N6 medium had a positive effect on anther culture performance.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Treatment
- Modified N6 mediume0
- Outcome
- anther culture performancee0
- Direction
- positive effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Indica Rice Varieties”
e0“Modified N6 medium had positive effect on anther culture performance”
Claim · model-written summary
The F1 hybrid Hu Low Tao × Bg 90-2 had the best anther culture performance.
- Taxon
- indica riceOryza sativa Indica Groupt0
- Material
- F1 hybrid Hu Low Tao × Bg 90-2e0
- Outcome
- best performancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Indica Rice Varieties”
e0“the F1 hybrid Hu Low Tao × Bg 90-2 had the best performance”
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