Abstract
Clusterbean (Cyamopsis tetragonoloba L. Taub) is a hardy crop and considered as green gold due to galactomannan content. Healthy seeds of clusterbean genotype GG-2 were surface sterilized and placed on medium containing B5 vitamins, 3% (w/v) sucrose, and 0.8% (w/v) bacteriological grade agar. The explants (cotyledon and cotyledonary nodes) obtained from seedlings of cluster bean (GG-2) were cultured in Murashige and Skoog's (MS) and B5 media supplemented with different concentrations of growth regulators NAA, BAP and 2, 4-D. Successful shoot inductions of GG-2 were established in B5 medium supplemented with NAA (2 mg/l) + BAP (2 mg/l). The cotyledonary nodes cultured on BA showed higher shoot morphogenesis capability compared to the cotyledons. The highest overall average shoot morphogenesis percentage (34%) for GG2 genotype was observed at 20μM BA. Subsequently, in the media supplemented with BA (14.4 μM) and IAA (12.3 μM), the cotyledonary node explants of cluster bean cultivars exhibited the highest percentage of multiple shoot formation. Highest percentage (74%) of rooting of in in vitro-regenerated shoots was recorded in B5 medium containing 3 mg/l IBA, 2 mg/L BA and 1 mg/l GA3 in the cluster bean genotype GG 2. The present regeneration system is efficient and can be used for genetic transformation studies.
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Claims 6
Claim · model-written summary
B5 medium with NAA and BAP established shoot induction in GG-2.
- Taxon
- Clusterbean
Cyamopsis tetragonolobae1 - Material
- GG-2e0
- Treatment
- B5 medium supplemented with NAA (2 mg/l) + BAP (2 mg/l)e0
- Outcome
- shoot inductionse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Successful shoot inductions of GG-2 were established in B5 medium supplemented with NAA (2 mg/l) + BAP (2 mg/l).”
e1“Clusterbean (Cyamopsis tetragonoloba L. Taub) is a hardy crop”
Claim · model-written summary
Cotyledonary nodes on BA had greater shoot morphogenesis capability than cotyledons.
- Taxon
- cluster beanCyamopsis tetragonolobae1
- Organ / stage
- cotyledonary nodese0
- Treatment
- BAe0
- Outcome
- shoot morphogenesis capabilitye0
- Direction
- highere0
- Comparator
- cotyledonse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The cotyledonary nodes cultured on BA showed higher shoot morphogenesis capability compared to the cotyledons.”
e1“cluster bean (GG-2)”
Claim · model-written summary
GG2 had maximum mean shoot morphogenesis of 34% at 20μM BA.
- Taxon
- cluster beanCyamopsis tetragonolobae1
- Material
- GG2e0
- Treatment
- 20μM BAe0
- Outcome
- shoot morphogenesis percentagee0
- Direction
- higheste0
- Value
- 34%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest overall average shoot morphogenesis percentage (34%) for GG2 genotype was observed at 20μM BA.”
e1“cluster bean (GG-2)”
Claim · model-written summary
BA and IAA medium gave the highest multiple shoot formation from cotyledonary nodes.
- Taxon
- cluster beanCyamopsis tetragonolobae0
- Organ / stage
- cotyledonary nodee0
- Treatment
- BA (14.4 μM) and IAA (12.3 μM)e0
- Outcome
- percentage of multiple shoot formatione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the media supplemented with BA (14.4 μM) and IAA (12.3 μM), the cotyledonary node explants of cluster bean cultivars exhibited the highest percentage of multiple shoot formation”
Claim · model-written summary
B5 medium with IBA, BA and GA3 gave the highest rooting, 74%, in GG 2.
- Taxon
- cluster beanCyamopsis tetragonolobae0
- Material
- GG 2e0
- Treatment
- B5 medium containing 3 mg/l IBA, 2 mg/L BA and 1 mg/l GA3e0
- Outcome
- rootinge0
- Direction
- Higheste0
- Value
- 74%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Highest percentage (74%) of rooting of in in vitro-regenerated shoots was recorded in B5 medium containing 3 mg/l IBA, 2 mg/L BA and 1 mg/l GA3 in the cluster bean genotype GG 2.”
Claim · model-written summary
The authors considered the regeneration system efficient and useful for transformation studies.
- Taxon
- cluster beanCyamopsis tetragonolobae1
- Outcome
- regeneration systeme0
- Direction
- efficiente0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The present regeneration system is efficient and can be used for genetic transformation studies.”
e1“cluster bean (GG-2)”
The same questions in other papers
Gibberellic acid · growth · Cyamopsis tetragonoloba (cluster bean)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.