Abstract
In this study, an Hsp70 gene was isolated from maize and was designated as ZmERD2 (Early Responsive to Dehydration 2). The full cDNA sequence of ZmERD2 was 2375 bp. It includes a 1949 bp open reading frame (ORF) encoding 649 amino acids. The calculated molecular mass was 71.163 kDa. The protein encoded by ZmERD2 contains an Hsp70 conservative structure domain, three typical Hsp70 family signature sequences and a cytosolic Hsp70-specific motif, which belongs to the Hsp70 family. Phylogenetic tree analysis suggested that ZmEDR2 shares high similarity (92%–95%) with Hsp70 of other plants, such as Arabidopsis thaliana (NP 195870), Triticum urartu (EMS52605), Aegilops tauschii (EMT07406) and Nicotiana tabacum (AAR17080), and was clustered together with T. urartu and A. tauschii. The promoter of ZmERD2, which was located at about 2.2 kb upstream of ZmERD2, was cloned and was predicted to contain important regulatory elements, including TATA-box, CAAT-box and drought-, heat-, cold-, salicylic acid- and methyl jasmonate-responsive elements. Analyzed by quantitative real-time polymerase chain reaction, the expression of ZmERD2 in maize was induced by heat, high-salt, cold, polyethylene glycol, heat stress and dehydration treatments, but not by abscisic acid. The expression of ZmEDR2 was quickly induced at 42 °C and reached its peak after 1 h of heat stress, with an expression of 40-fold above the control level. These results suggested that ZmERD2 might be a stress-related gene in maize.
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Claims 13
Claim · model-written summary
A maize Hsp70 gene was isolated and designated ZmERD2.
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an Hsp70 gene was isolated from maize and was designated as ZmERD2”
Claim · model-written summary
ZmERD2 full cDNA is 2375 bp.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The full cDNA sequence of ZmERD2 was 2375 bp”
e1“isolated from maize”
Claim · model-written summary
ZmERD2 has a 1949 bp open reading frame.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It includes a 1949 bp open reading frame (ORF) encoding 649 amino acids”
e1“isolated from maize”
e2“designated as ZmERD2”
Claim · model-written summary
The ZmERD2 open reading frame encodes 649 amino acids.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It includes a 1949 bp open reading frame (ORF) encoding 649 amino acids”
e1“isolated from maize”
e2“designated as ZmERD2”
Claim · model-written summary
The predicted ZmERD2 molecular mass is 71.163 kDa.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The calculated molecular mass was 71.163 kDa”
e1“isolated from maize”
e2“designated as ZmERD2”
Claim · model-written summary
ZmERD2 protein has an Hsp70 domain and characteristic family motifs.
- Gene
- ZmERD2e0
- Outcome
- Hsp70 conservative structure domain, three typical Hsp70 family signature sequences and a cytosolic Hsp70-specific motife0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The protein encoded by ZmERD2 contains an Hsp70 conservative structure domain, three typical Hsp70 family signature sequences and a cytosolic Hsp70-specific motif”
e1“isolated from maize”
Claim · model-written summary
Phylogenetic analysis found 92%–95% similarity to Hsp70 from other plants.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ZmEDR2 shares high similarity (92%–95%) with Hsp70 of other plants”
e1“isolated from maize”
Claim · model-written summary
ZmEDR2 clustered with T. urartu and A. tauschii.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was clustered together with T. urartu and A. tauschii”
e1“isolated from maize”
e2“ZmEDR2 shares”
Claim · model-written summary
The ZmERD2 promoter lies about 2.2 kb upstream.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The promoter of ZmERD2, which was located at about 2.2 kb upstream of ZmERD2, was cloned”
e1“isolated from maize”
Claim · model-written summary
The promoter is predicted to have TATA-box, CAAT-box and stress-responsive elements.
- Gene
- ZmERD2e2
- Outcome
- TATA-box, CAAT-box and drought-, heat-, cold-, salicylic acid- and methyl jasmonate-responsive elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“was predicted to contain important regulatory elements, including TATA-box, CAAT-box and drought-, heat-, cold-, salicylic acid- and methyl jasmonate-responsive elements”
e1“isolated from maize”
e2“designated as ZmERD2”
Claim · model-written summary
Several stress treatments induced ZmERD2 expression in maize.
- Treatment
- heat, high-salt, cold, polyethylene glycol, heat stress and dehydration treatmentse0
- Gene
- ZmERD2e0
- Outcome
- expression of ZmERD2e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of ZmERD2 in maize was induced by heat, high-salt, cold, polyethylene glycol, heat stress and dehydration treatments”
Claim · model-written summary
Abscisic acid did not induce ZmERD2 expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of ZmERD2 in maize was induced by heat, high-salt, cold, polyethylene glycol, heat stress and dehydration treatments, but not by abscisic acid”
Claim · model-written summary
At 42 °C ZmEDR2 expression peaked after 1 h at 40-fold control.
- Treatment
- 42 °Ce0
- Gene
- ZmEDR2e0
- Outcome
- expression of ZmEDR2e0
- Direction
- abovee0
- Value
- 40-folde0
- Qualifier
- after 1 h of heat stresse0
- Comparator
- control levele0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression of ZmEDR2 was quickly induced at 42 °C and reached its peak after 1 h of heat stress, with an expression of 40-fold above the control level”
e1“isolated from maize”
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See all 65 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.