Abstract
The Magnaporthe oryzae snodprot1 homolog (MSP1), secreted by M. oryzae, is a cerato-platanin family protein. msp1-knockout mutants have reduced virulence on barley leaves, indicating that MSP1 is required for the pathogenicity of rice blast fungus. To investigate the functional roles of MSP1 and its downstream signaling in rice, recombinant MSP1 was produced in Escherichia coli and was assayed for its functionality. Application of MSP1 triggered cell death and elicited defense responses in rice. MSP1 also induced H2O2 production and autophagic cell death in both suspension-cultured cells and rice leaves. One or more protein kinases triggered cell death, jasmonic acid and abscisic acid enhanced cell death, while salicylic acid suppressed it. We demonstrated that the secretion of MSP1 into the apoplast is a prerequisite for triggering cell death and activating defense-related gene expression. Furthermore, pretreatment of rice with a sublethal MSP1 concentration potentiated resistance to the pathogen. Taken together, our results showed that MSP1 induces a high degree of cell death in plants, which might be essential for its virulence. Moreover, rice can recognize MSP1, resulting in the induction of pathogen-associated molecular pattern-triggered immunity.
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Claims 11
Claim · model-written summary
msp1 knockout mutants had reduced virulence on barley leaves.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Organ / stage
- leavese0
- Gene
- msp1e0
- Outcome
- virulence on barley leavese0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“msp1-knockout mutants have reduced virulence on barley leaves”
Claim · model-written summary
Applied MSP1 triggered cell death and defense responses in rice.
- Taxon
- rice
Oryza sativae0 - Treatment
- Application of MSP1e0
- Gene
- MSP1e0
- Outcome
- cell death and elicited defense responsese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Application of MSP1 triggered cell death and elicited defense responses in rice”
Claim · model-written summary
MSP1 induced H2O2 production in rice cells and leaves.
- Taxon
- riceOryza sativae0
- Organ / stage
- suspension-cultured cells and rice leavese0
- Treatment
- MSP1e0
- Outcome
- H2O2 productione0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MSP1 also induced H2O2 production and autophagic cell death in both suspension-cultured cells and rice leaves”
Claim · model-written summary
MSP1 induced autophagic cell death in rice cells and leaves.
- Taxon
- riceOryza sativae0
- Organ / stage
- suspension-cultured cells and rice leavese0
- Treatment
- MSP1e0
- Outcome
- autophagic cell deathe0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MSP1 also induced H2O2 production and autophagic cell death in both suspension-cultured cells and rice leaves”
Claim · model-written summary
One or more protein kinases triggered cell death.
- Taxon
- riceOryza sativat0
- Gene
- protein kinasese0
- Outcome
- cell deathe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One or more protein kinases triggered cell death”
t0“rice”
Claim · model-written summary
Jasmonic acid enhanced cell death.
- Taxon
- riceOryza sativat0
- Treatment
- jasmonic acide0
- Outcome
- cell deathe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“jasmonic acid and abscisic acid enhanced cell death”
t0“rice”
Claim · model-written summary
Abscisic acid enhanced cell death.
- Taxon
- riceOryza sativat0
- Treatment
- abscisic acide0
- Outcome
- cell deathe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“jasmonic acid and abscisic acid enhanced cell death”
t0“rice”
Claim · model-written summary
Salicylic acid suppressed cell death.
- Taxon
- riceOryza sativat0
- Treatment
- salicylic acide0
- Outcome
- cell deathe0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“One or more protein kinases triggered cell death, jasmonic acid and abscisic acid enhanced cell death, while salicylic acid suppressed it”
t0“rice”
Claim · model-written summary
MSP1 secretion into the apoplast was required to trigger cell death and defense gene expression.
- Taxon
- riceOryza sativat0
- Gene
- MSP1e0
- Outcome
- cell death and activating defense-related gene expressione0
- Qualifier
- into the apoplaste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the secretion of MSP1 into the apoplast is a prerequisite for triggering cell death and activating defense-related gene expression”
t0“rice”
Claim · model-written summary
Sublethal MSP1 pretreatment potentiated rice resistance to the pathogen.
- Taxon
- riceOryza sativae0
- Treatment
- pretreatment of rice with a sublethal MSP1 concentratione0
- Outcome
- resistance to the pathogene0
- Direction
- potentiatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“pretreatment of rice with a sublethal MSP1 concentration potentiated resistance to the pathogen”
Claim · model-written summary
Authors infer that rice recognition of MSP1 induces pattern triggered immunity.
- Taxon
- riceOryza sativae0
- Gene
- MSP1e0
- Outcome
- pathogen-associated molecular pattern-triggered immunitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice can recognize MSP1, resulting in the induction of pathogen-associated molecular pattern-triggered immunity”
The same questions in other papers
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6 papers in the same direction as this paper · 6 in the opposite direction
- positive
- negative
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9 papers more in this group
See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.