Abstract
Treatment of suspension-cultured cells of rice (Oryza sativa L.) with cell wall extract of rice blast fungus (Magnaporthe grisea) elicits a rapid generation of H2O2, alkalinization of culture medium, and eventual cell death. To elucidate genes involved in these processes, we exploited SAGE (Serial Analysis of Gene Expression) technique for the molecular analysis of cell death in suspension-cultured cells treated with the elicitor. Among the downregulated genes in the elicitor-treated cells, a BI-1 gene coding for Bax inhibitor was identified. Transgenic rice cells overexpressing Arabidopsis BI-1 gene showed sustainable cell survival when challenged with M. grisea elicitor. Thus, the plant Bax inhibitor plays a functional role in regulating cell death in the rice cell culture system.
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Claims 3
Claim · model-written summary
Fungal cell wall extract elicited H2O2 generation, medium alkalinization and cell death in rice cells.
- Taxon
- rice
Oryza sativae0 - Treatment
- cell wall extract of rice blast fungus (Magnaporthe grisea)e0
- Interactor
- Magnaporthe grisea
Pyricularia griseae0pathogen - Outcome
- rapid generation of H2O2, alkalinization of culture medium, and eventual cell deathe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of suspension-cultured cells of rice (Oryza sativa L.) with cell wall extract of rice blast fungus (Magnaporthe grisea) elicits a rapid generation of H2O2, alkalinization of culture medium, and eventual cell death”
Claim · model-written summary
BI-1 was downregulated in elicitor-treated rice cells.
- Taxon
- riceOryza sativat0
- Treatment
- elicitore0
- Gene
- BI-1e0
- Outcome
- downregulated genese0
- Direction
- downregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the downregulated genes in the elicitor-treated cells, a BI-1 gene coding for Bax inhibitor was identified”
t0“rice (Oryza sativa L.)”
Claim · model-written summary
Rice cells overexpressing Arabidopsis BI-1 survived fungal elicitor challenge.
- Taxon
- riceOryza sativae0
- Treatment
- overexpressing Arabidopsis BI-1 genee0
- Interactor
- M. griseaPyricularia griseae0pathogen
- Gene
- Arabidopsis BI-1e0
- Outcome
- sustainable cell survivale0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic rice cells overexpressing Arabidopsis BI-1 gene showed sustainable cell survival when challenged with M. grisea elicitor”
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