Abstract
Currently, only few glufosinate-resistant genes are available for commercial application. Thus, developing novel glufosinate-resistant genes with commercial feasibility is extremely important and urgent for agricultural production. In this study, we transferred a newly isolated RePAT gene into a japonica rice variety Zhonghua11, resulting in a large number of independent T0 transgenic plants, most of which grew normally under high-concentration glufosinate treatment. Four transgenic plants with one intact RePAT expression cassette integrated into the intergenic region were selected. Agronomic performances of their T2 progenies were investigated, and the results suggested that the expression of RePAT had no adverse effect on the agronomic performance. Definite glufosinate resistance of the selected transgenic plants was further confirmed to be related to the expression of RePAT by assay on the medium and qRT-PCR. The inheritance and expression of RePAT in two transgenic plants were confirmed to be stable. Finally, the two-year field assay of glufosinate resistance suggested that the agronomic performance of the transgenic plant (PAT11) was not affected by high dosage of glufosinate (5000 g/ha). Collectively, our study proves the high resistance of a novel gene RePAT to glufosinate and provides a glufosiante-resistant rice variety with agricultural application potential.
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Claims 6
Claim · model-written summary
Most independent RePAT transgenic rice plants grew normally under high-concentration glufosinate.
- Taxon
- rice
Oryza sativat0 - Treatment
- high-concentration glufosinate treatmente0
- Outcome
- grew normallye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most of which grew normally under high-concentration glufosinate treatment.”
t0“rice”
Claim · model-written summary
Four selected plants carried one intact RePAT expression cassette in an intergenic region.
- Taxon
- riceOryza sativat0
- Gene
- RePATe0
- Outcome
- one intact RePAT expression cassette integrated into the intergenic regione0
- Value
- Four transgenic plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Four transgenic plants with one intact RePAT expression cassette integrated into the intergenic region were selected.”
t0“rice”
Claim · model-written summary
RePAT expression had no adverse effect on agronomic performance of T2 progeny.
- Taxon
- riceOryza sativat0
- Treatment
- expression of RePATe0
- Gene
- RePATe0
- Outcome
- agronomic performancee0
- Direction
- no adverse effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of RePAT had no adverse effect on the agronomic performance.”
t0“rice”
Claim · model-written summary
Selected transgenic plants showed glufosinate resistance associated with RePAT expression.
Not reportedTaxonDirection
Verbatim
e0“Definite glufosinate resistance of the selected transgenic plants was further confirmed to be related to the expression of RePAT by assay on the medium and qRT-PCR.”
t0“rice”
Claim · model-written summary
Inheritance and expression of RePAT were stable in two transgenic plants.
- Taxon
- riceOryza sativat0
- Gene
- RePATe0
- Outcome
- inheritance and expression of RePATe0
- Direction
- stablee0
- Value
- two transgenic plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The inheritance and expression of RePAT in two transgenic plants were confirmed to be stable.”
t0“rice”
Claim · model-written summary
A two-year field assay found PAT11 agronomic performance unaffected by 5000 g/ha glufosinate.
- Taxon
- riceOryza sativat0
- Material
- PAT11e0
- Treatment
- high dosage of glufosinate (5000 g/ha)e0
- Outcome
- agronomic performancee0
- Direction
- not affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the agronomic performance of the transgenic plant (PAT11) was not affected by high dosage of glufosinate (5000 g/ha).”
t0“rice”
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