Paper · 2016 · TURKISH JOURNAL OF AGRICULTURE AND FORESTRY

Foliar spray of selected plant growth regulators affected the biochemical and antioxidant attributes of spinach in a field experiment

Maryam ASLAM; Bushra SULTANA; Farooq ANWAR; et al.

10
claims
20
verbatim quotes
69
citations
2
research areas
✓
Open access

Abstract

This study explored the effects of foliar spray of selected plant growth regulators (PGRs) on the biochemical and phenolic antioxidant attributes of spinach (Spinacia oleracea) leaves. Following foliar spray at three growth stages (40, 50, and 60 days) with selected PGRs, namely humic acid (HA), Moringa oleifera leaf extract (MOLE), and 6-benzylamino purine (6-BAP), and their mixed form, the samples of spinach leaves were harvested. The analysis of foliar-treated leaves revealed the content of proline, malondialdehyde (MDA), total soluble proteins (TSPs), total chlorophyll, and total carotenoids to be 22.45-72.32 μg/g FW, 2.41-29.13 ng/g FW, 3.21-22.45 μg/g FW, 4.173-19.700 mg/g FW, and 0.500-1.260 mg/g FW, respectively. The total phenolic content (TPC), reducing power, and DPPH° scavenging activity (IC50 values) varied from 6.59 to 14.49 mg GAE/g DM, from 0.426 to 1.944 (10.0 mg/mL extract concentration), and from 0.506 to 1.073 μg/mL, respectively. HPLC analysis showed that foliar application of PGRs also improved the content of individual phenolic acids in the leaves as compared with the controls. Overall, it was concluded that, although the use of the PGRs acted as an elicitor with respect to each growth phase and exhibited significant differences (P < 0.05) among treatments, the leading effect was exhibited by the MOLE treatment, proving it to be a better enhancer of phenolic antioxidants and other biochemicals.

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Claims 10

Observationobservedoriginal resultmeasured

Claim · model-written summary

Treated spinach leaves had proline content of 22.45-72.32 μg/g FW.

Outcome
prolinee0
Value
22.45-72.32 μg/g FWe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the content of proline, malondialdehyde (MDA), total soluble proteins (TSPs), total chlorophyll, and total carotenoids to be 22.45-72.32 μg/g FW, 2.41-29.13 ng/g FW, 3.21-22.45 μg/g FW, 4.173-19.700 mg/g FW, and 0.500-1.260 mg/g FW, respectively”

✓ located verbatim in the source · abstract, characters 484–729 · show in text ↑
e1

“This study explored the effects of foliar spray of selected plant growth regulators (PGRs) on the biochemical and phenolic antioxidant attributes of spinach (Spinacia oleracea) leaves.”

✓ located verbatim in the source · abstract, characters 0–184 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Treated spinach leaves had MDA content of 2.41-29.13 ng/g FW.

Outcome
malondialdehyde (MDA)e0
Value
2.41-29.13 ng/g FWe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the content of proline, malondialdehyde (MDA), total soluble proteins (TSPs), total chlorophyll, and total carotenoids to be 22.45-72.32 μg/g FW, 2.41-29.13 ng/g FW, 3.21-22.45 μg/g FW, 4.173-19.700 mg/g FW, and 0.500-1.260 mg/g FW, respectively”

✓ located verbatim in the source · abstract, characters 484–729 · show in text ↑
e1

“This study explored the effects of foliar spray of selected plant growth regulators (PGRs) on the biochemical and phenolic antioxidant attributes of spinach (Spinacia oleracea) leaves.”

✓ located verbatim in the source · abstract, characters 0–184 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Treated spinach leaves had TSP content of 3.21-22.45 μg/g FW.

Outcome
total soluble proteins (TSPs)e0
Value
3.21-22.45 μg/g FWe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the content of proline, malondialdehyde (MDA), total soluble proteins (TSPs), total chlorophyll, and total carotenoids to be 22.45-72.32 μg/g FW, 2.41-29.13 ng/g FW, 3.21-22.45 μg/g FW, 4.173-19.700 mg/g FW, and 0.500-1.260 mg/g FW, respectively”

✓ located verbatim in the source · abstract, characters 484–729 · show in text ↑
e1

“This study explored the effects of foliar spray of selected plant growth regulators (PGRs) on the biochemical and phenolic antioxidant attributes of spinach (Spinacia oleracea) leaves.”

✓ located verbatim in the source · abstract, characters 0–184 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Treated spinach leaves had chlorophyll content of 4.173-19.700 mg/g FW.

Outcome
total chlorophylle0
Value
4.173-19.700 mg/g FWe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the content of proline, malondialdehyde (MDA), total soluble proteins (TSPs), total chlorophyll, and total carotenoids to be 22.45-72.32 μg/g FW, 2.41-29.13 ng/g FW, 3.21-22.45 μg/g FW, 4.173-19.700 mg/g FW, and 0.500-1.260 mg/g FW, respectively”

✓ located verbatim in the source · abstract, characters 484–729 · show in text ↑
e1

“This study explored the effects of foliar spray of selected plant growth regulators (PGRs) on the biochemical and phenolic antioxidant attributes of spinach (Spinacia oleracea) leaves.”

✓ located verbatim in the source · abstract, characters 0–184 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Treated spinach leaves had carotenoid content of 0.500-1.260 mg/g FW.

Outcome
total carotenoidse0
Value
0.500-1.260 mg/g FWe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the content of proline, malondialdehyde (MDA), total soluble proteins (TSPs), total chlorophyll, and total carotenoids to be 22.45-72.32 μg/g FW, 2.41-29.13 ng/g FW, 3.21-22.45 μg/g FW, 4.173-19.700 mg/g FW, and 0.500-1.260 mg/g FW, respectively”

✓ located verbatim in the source · abstract, characters 484–729 · show in text ↑
e1

“This study explored the effects of foliar spray of selected plant growth regulators (PGRs) on the biochemical and phenolic antioxidant attributes of spinach (Spinacia oleracea) leaves.”

✓ located verbatim in the source · abstract, characters 0–184 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Total phenolic content varied from 6.59 to 14.49 mg GAE/g DM.

Outcome
total phenolic content (TPC)e0
Value
6.59 to 14.49 mg GAE/g DMe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The total phenolic content (TPC), reducing power, and DPPH° scavenging activity (IC50 values) varied from 6.59 to 14.49 mg GAE/g DM, from 0.426 to 1.944 (10.0 mg/mL extract concentration), and from 0.506 to 1.073 μg/mL, respectively”

✓ located verbatim in the source · abstract, characters 731–963 · show in text ↑
e1

“This study explored the effects of foliar spray of selected plant growth regulators (PGRs) on the biochemical and phenolic antioxidant attributes of spinach (Spinacia oleracea) leaves.”

✓ located verbatim in the source · abstract, characters 0–184 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Reducing power varied from 0.426 to 1.944.

Outcome
reducing powere0
Value
0.426 to 1.944e0
Qualifier
10.0 mg/mL extract concentratione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The total phenolic content (TPC), reducing power, and DPPH° scavenging activity (IC50 values) varied from 6.59 to 14.49 mg GAE/g DM, from 0.426 to 1.944 (10.0 mg/mL extract concentration), and from 0.506 to 1.073 μg/mL, respectively”

✓ located verbatim in the source · abstract, characters 731–963 · show in text ↑
e1

“This study explored the effects of foliar spray of selected plant growth regulators (PGRs) on the biochemical and phenolic antioxidant attributes of spinach (Spinacia oleracea) leaves.”

✓ located verbatim in the source · abstract, characters 0–184 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

DPPH scavenging IC50 varied from 0.506 to 1.073 μg/mL.

Outcome
DPPH° scavenging activity (IC50 values)e0
Value
0.506 to 1.073 μg/mLe0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The total phenolic content (TPC), reducing power, and DPPH° scavenging activity (IC50 values) varied from 6.59 to 14.49 mg GAE/g DM, from 0.426 to 1.944 (10.0 mg/mL extract concentration), and from 0.506 to 1.073 μg/mL, respectively”

✓ located verbatim in the source · abstract, characters 731–963 · show in text ↑
e1

“This study explored the effects of foliar spray of selected plant growth regulators (PGRs) on the biochemical and phenolic antioxidant attributes of spinach (Spinacia oleracea) leaves.”

✓ located verbatim in the source · abstract, characters 0–184 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Foliar PGR application improved individual phenolic acid content over controls.

Organ / stage
leavese0
Treatment
foliar application of PGRse0
Outcome
content of individual phenolic acidse0
Direction
improvede0
Comparator
the controlse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“foliar application of PGRs also improved the content of individual phenolic acids in the leaves as compared with the controls”

✓ located verbatim in the source · abstract, characters 991–1116 · show in text ↑
e1

“This study explored the effects of foliar spray of selected plant growth regulators (PGRs) on the biochemical and phenolic antioxidant attributes of spinach (Spinacia oleracea) leaves.”

✓ located verbatim in the source · abstract, characters 0–184 · show in text ↑
Intervention effectauthor interpretationoriginal resultpositivemeasured

Claim · model-written summary

MOLE had the leading effect in enhancing phenolic antioxidants and other biochemicals.

Treatment
MOLE treatmente0
Outcome
phenolic antioxidants and other biochemicalse0
Direction
better enhancere0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Spinacia oleracea
Spinacia oleracea · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“the leading effect was exhibited by the MOLE treatment, proving it to be a better enhancer of phenolic antioxidants and other biochemicals”

✓ located verbatim in the source · abstract, characters 1301–1439 · show in text ↑
e1

“This study explored the effects of foliar spray of selected plant growth regulators (PGRs) on the biochemical and phenolic antioxidant attributes of spinach (Spinacia oleracea) leaves.”

✓ located verbatim in the source · abstract, characters 0–184 · show in text ↑

application · quality: phytochemicals · Spinacia oleracea (spinach)Positive and negative results

6 papers: 5 positive, 1 negative or no effect

4 papers in the same direction as this paper · 1 in the opposite direction

  1. no effect
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

class · quality: phytochemicals · Spinacia oleracea (spinach)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

botanical · quality: phytochemicals · Spinacia oleracea (spinach)Results agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.