Abstract
The Bcl-2-associated athanogene (BAG) family is an evolutionarily conserved group of cochaperones that modulate numerous cellular processes. Previously we found that Arabidopsis thaliana BAG6 is required for basal immunity against the fungal phytopathogen Botrytis cinerea. However, the mechanisms by which BAG6 controls immunity are obscure. Here, we address this important question by determining the molecular mechanisms responsible for BAG6-mediated basal resistance. We show that Arabidopsis BAG6 is cleaved in vivo in a caspase-1-like-dependent manner and via a combination of pull-downs, mass spectrometry, yeast two-hybrid assays, and chemical genomics, we demonstrate that BAG6 interacts with a C2 GRAM domain protein (BAGP1) and an aspartyl protease (APCB1), both of which are required for BAG6 processing. Furthermore, fluorescence and transmission electron microscopy established that BAG6 cleavage triggers autophagy in the host that coincides with disease resistance. Targeted inactivation of BAGP1 or APCB1 results in the blocking of BAG6 processing and loss of resistance. Mutation of the cleavage site blocks cleavage and inhibits autophagy in plants; disease resistance is also compromised. Taken together, these results identify a mechanism that couples an aspartyl protease with a molecular cochaperone to trigger autophagy and plant defense, providing a key link between fungal recognition and the induction of cell death and resistance.
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Claims 8
Claim · model-written summary
Arabidopsis BAG6 is cleaved in vivo in a caspase-1-like-dependent manner.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- BAG6e0
- Outcome
- cleaved in vivoe0
- Qualifier
- caspase-1-like-dependente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We show that Arabidopsis BAG6 is cleaved in vivo in a caspase-1-like-dependent manner”
Claim · model-written summary
BAG6 interacts with the C2 GRAM domain protein BAGP1, which is required for BAG6 processing.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- BAGP1e1
- Outcome
- BAG6 processinge1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis BAG6”
e1“BAG6 interacts with a C2 GRAM domain protein (BAGP1) and an aspartyl protease (APCB1), both of which are required for BAG6 processing”
Claim · model-written summary
BAG6 interacts with the aspartyl protease APCB1, which is required for BAG6 processing.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- APCB1e1
- Outcome
- BAG6 processinge1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis BAG6”
e1“BAG6 interacts with a C2 GRAM domain protein (BAGP1) and an aspartyl protease (APCB1), both of which are required for BAG6 processing”
Claim · model-written summary
BAG6 cleavage triggers autophagy in the host, coinciding with disease resistance.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- BAG6e1
- Outcome
- autophagye1
- Qualifier
- coincides with disease resistancee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis BAG6”
e1“BAG6 cleavage triggers autophagy in the host that coincides with disease resistance”
Claim · model-written summary
Targeted inactivation of BAGP1 or APCB1 blocks BAG6 processing.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Targeted inactivation of BAGP1 or APCB1e1
- Outcome
- BAG6 processinge1
- Direction
- blockinge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis BAG6”
e1“Targeted inactivation of BAGP1 or APCB1 results in the blocking of BAG6 processing and loss of resistance”
Claim · model-written summary
Targeted inactivation of BAGP1 or APCB1 results in loss of resistance.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Targeted inactivation of BAGP1 or APCB1e1
- Outcome
- resistancee1
- Direction
- losse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis BAG6”
e1“Targeted inactivation of BAGP1 or APCB1 results in the blocking of BAG6 processing and loss of resistance”
Claim · model-written summary
Mutation of the BAG6 cleavage site blocks cleavage and inhibits autophagy in plants.
- Taxon
- plantsViridiplantaee0
- Treatment
- Mutation of the cleavage sitee0
- Outcome
- cleavage and inhibits autophagye0
- Direction
- blockse0
Not reportedComparator
Verbatim
e0“Mutation of the cleavage site blocks cleavage and inhibits autophagy in plants”
Claim · model-written summary
Mutation of the cleavage site also compromises disease resistance.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Mutation of the cleavage sitee1
- Outcome
- disease resistancee1
- Direction
- compromisede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis BAG6”
e1“Mutation of the cleavage site blocks cleavage and inhibits autophagy in plants; disease resistance is also compromised”
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