Abstract
Plant stem cells are hypersensitive to environmental hazards throughout their life cycle, but the mechanism by which plants safeguard stem cell homeostasis in response to environmental hazards is largely unknown. The homeodomain transcription factor WUSCHEL (WUS) protein maintains the stem cell pool in the shoot apical meristem of Arabidopsis. Here, we demonstrate that the translation of WUS mRNA is directed by an internal ribosomal entry site (IRES) located in the 5'-untranslated region. The AtLa1 protein, an RNA-binding factor, binds to the 5'-untranslated region and initiates the IRES-dependent translation of WUS mRNA. Knockdown of AtLa1 expression represses the WUS IRES-dependent translation and leads to the arrest of growth and development. The AtLa1 protein is mainly located in the nucleoplasm. However, environmental hazards promote the nuclear-to-cytoplasmic translocation of the AtLa1 protein, which further enhances the IRES-dependent translation of WUS mRNA. Genetic evidence indicates that the WUS protein increases the tolerance of the shoot apical meristem to environmental hazards. Based on these results, we conclude that the stem cell niche in Arabidopsis copes with environmental hazards by enhancing the IRES-dependent translation of WUS mRNA under the control of the AtLa1 protein.
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Claims 8
Claim · model-written summary
The AtLa1 protein is mainly located in the nucleoplasm of Arabidopsis cells.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- AtLa1e1
- Outcome
- mainly located in the nucleoplasme1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maintains the stem cell pool in the shoot apical meristem of Arabidopsis”
e1“The AtLa1 protein is mainly located in the nucleoplasm”
Claim · model-written summary
The translation of WUS mRNA is directed by an internal ribosomal entry site located in its 5'-untranslated region.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- translation of WUS mRNAe2
- Qualifier
- internal ribosomal entry site (IRES) located in the 5'-untranslated regione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maintains the stem cell pool in the shoot apical meristem of Arabidopsis”
e2“the translation of WUS mRNA is directed by an internal ribosomal entry site (IRES) located in the 5'-untranslated region”
Claim · model-written summary
AtLa1 protein binds the 5'-untranslated region and initiates IRES-dependent translation of WUS mRNA.
- Taxon
- ArabidopsisArabidopsise0
- Gene
- AtLa1e3
- Outcome
- IRES-dependent translation of WUS mRNAe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maintains the stem cell pool in the shoot apical meristem of Arabidopsis”
e3“The AtLa1 protein, an RNA-binding factor, binds to the 5'-untranslated region and initiates the IRES-dependent translation of WUS mRNA”
Claim · model-written summary
Knockdown of AtLa1 represses WUS IRES-dependent translation in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Knockdown of AtLa1 expressione4
- Gene
- AtLa1e4
- Outcome
- WUS IRES-dependent translatione4
- Direction
- repressese4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maintains the stem cell pool in the shoot apical meristem of Arabidopsis”
e4“Knockdown of AtLa1 expression represses the WUS IRES-dependent translation and leads to the arrest of growth and development”
Claim · model-written summary
Knockdown of AtLa1 leads to arrest of growth and development in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- Knockdown of AtLa1 expressione4
- Outcome
- arrest of growth and developmente4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maintains the stem cell pool in the shoot apical meristem of Arabidopsis”
e4“Knockdown of AtLa1 expression represses the WUS IRES-dependent translation and leads to the arrest of growth and development”
Claim · model-written summary
Environmental hazards promote nuclear-to-cytoplasmic translocation of the AtLa1 protein.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- environmental hazardse5
- Gene
- AtLa1e5
- Outcome
- nuclear-to-cytoplasmic translocation of the AtLa1 proteine5
- Direction
- promotee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maintains the stem cell pool in the shoot apical meristem of Arabidopsis”
e5“However, environmental hazards promote the nuclear-to-cytoplasmic translocation of the AtLa1 protein, which further enhances the IRES-dependent translation of WUS mRNA”
Claim · model-written summary
Environmental hazards further enhance IRES-dependent translation of WUS mRNA.
- Taxon
- ArabidopsisArabidopsise0
- Treatment
- environmental hazardse5
- Gene
- WUSe5
- Outcome
- IRES-dependent translation of WUS mRNAe5
- Direction
- enhancese5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maintains the stem cell pool in the shoot apical meristem of Arabidopsis”
e5“However, environmental hazards promote the nuclear-to-cytoplasmic translocation of the AtLa1 protein, which further enhances the IRES-dependent translation of WUS mRNA”
Claim · model-written summary
The WUS protein increases the tolerance of the shoot apical meristem to environmental hazards in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- shoot apical meristeme6
- Treatment
- environmental hazardse6
- Gene
- WUS proteine6
- Outcome
- tolerance of the shoot apical meristeme6
- Direction
- increasese6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“maintains the stem cell pool in the shoot apical meristem of Arabidopsis”
e6“Genetic evidence indicates that the WUS protein increases the tolerance of the shoot apical meristem to environmental hazards”
The same questions in other papers
climate site · disease · ArabidopsisPositive and negative results
2 in the opposite direction
- negative
- negative
climate site · molecular: expression · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 8 in the opposite direction
- negative
- no effect
- no effect
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.