Abstract
The WRKY transcription factors have been demonstrated to play crucial roles in regulating stress responses; however, the exact mechanisms underlying their involvement in stress responses are not fully understood. Arabidopsis WRKY8 was predominantly expressed in roots and was highly upregulated by salt treatment. Disruption of WRKY8 rendered plants hypersensitive to salt, showing delayed germination, inhibited post-germination development and accelerated chlorosis. Further investigation revealed that WRKY8 interacted with VQ9, and their interaction decreased the DNA-binding activity of WRKY8. The VQ9 protein was exclusively localized in the nucleus, and VQ9 expression was strongly responsive to NaCl treatment. Mutation of VQ9 enhanced tolerance to salt stress, indicating that VQ9 acts antagonistically with WRKY8 to mediate responses to salt stress. The antagonist functions of WRKY8 and VQ9 were consistent with an increased or reduced Na⁺/K⁺ concentration ratio, as well as contrasting expression patterns of downstream stress-responsive genes in salt-stressed wrky8 and vq9 mutants. Moreover, chromatin immunoprecipitation (ChIP) assays showed that WRKY8 directly bound the promoter of RD29A under salt conditions. These results provided strong evidence that the VQ9 protein acts as a repressor of the WRKY8 factor to maintain an appropriate balance of WRKY8-mediated signaling pathways to establish salinity stress tolerance.
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Claims 7
Claim · model-written summary
WRKY8 was predominantly expressed in roots and upregulated by salt.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- rootse0
- Treatment
- salt treatmente0
- Gene
- WRKY8e0
- Outcome
- expressed in roots and was highly upregulatede0
- Direction
- upregulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis WRKY8 was predominantly expressed in roots and was highly upregulated by salt treatment.”
Claim · model-written summary
WRKY8 disruption caused salt hypersensitivity, delayed germination, inhibited development and accelerated chlorosis.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Disruption of WRKY8e0
- Gene
- WRKY8e0
- Outcome
- hypersensitive to salt, showing delayed germination, inhibited post-germination development and accelerated chlorosise0
- Direction
- hypersensitivee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Disruption of WRKY8 rendered plants hypersensitive to salt, showing delayed germination, inhibited post-germination development and accelerated chlorosis.”
t0“Arabidopsis”
Claim · model-written summary
WRKY8 interacted with VQ9 and this reduced WRKY8 DNA binding.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- WRKY8e0
- Outcome
- DNA-binding activity of WRKY8e0
- Direction
- decreasede0
- Qualifier
- interacted with VQ9e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WRKY8 interacted with VQ9, and their interaction decreased the DNA-binding activity of WRKY8.”
t0“Arabidopsis”
Claim · model-written summary
VQ9 localized in nuclei and responded to NaCl.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- NaCl treatmente0
- Gene
- VQ9e0
- Outcome
- exclusively localized in the nucleus, and VQ9 expression was strongly responsivee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The VQ9 protein was exclusively localized in the nucleus, and VQ9 expression was strongly responsive to NaCl treatment.”
t0“Arabidopsis”
Claim · model-written summary
VQ9 mutation enhanced salt tolerance.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Mutation of VQ9e0
- Gene
- VQ9e0
- Outcome
- tolerance to salt stresse0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutation of VQ9 enhanced tolerance to salt stress”
t0“Arabidopsis”
Claim · model-written summary
WRKY8 and VQ9 mutants showed contrasting Na⁺/K⁺ ratios and stress gene expression under salt.
- Taxon
- ArabidopsisArabidopsist0
- Material
- wrky8 and vq9 mutantse0
- Gene
- WRKY8e0
- Outcome
- Na⁺/K⁺ concentration ratio, as well as contrasting expression patterns of downstream stress-responsive genese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The antagonist functions of WRKY8 and VQ9 were consistent with an increased or reduced Na⁺/K⁺ concentration ratio, as well as contrasting expression patterns of downstream stress-responsive genes in salt-stressed wrky8 and vq9 mutants.”
t0“Arabidopsis”
Claim · model-written summary
WRKY8 bound the RD29A promoter under salt.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- WRKY8e0
- Outcome
- bound the promoter of RD29Ae0
- Qualifier
- under salt conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WRKY8 directly bound the promoter of RD29A under salt conditions.”
t0“Arabidopsis”
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