Abstract
Verticillium wilt is one of the main diseases in cotton (Gossypium hirsutum), severely reduces yield and fiber quality, and is difficult to be con-trolled effectively. At present, the molecular mechanism that confers resistance to this disease is unclear. Transcriptome sequencing is an important method to detect resistance genes, explore metabolic pathways, and study resistance mechanisms. In this study, the transcriptome of a disease-resistant inbred cot-ton line inoculated with Verticillium dahliae was sequenced. A total of 126,402 unigenes were obtained using de novo assembly and data analysis, 99,712 (78.88%) of which were annotated into the Nr, Nt, Swiss-Prot, KEGG, COG, and GO databases. The expression patterns of 16 candidate disease-resis-tance genes showed that some genes were upregulated soon after V. dahliae inoculation and others were upregulated later, which may indicate instanta-neous basal defense and lagged specific defense, respectively. We conducted a preliminary analysis of the transcriptome database, which will contribute to further research regarding the cloning of disease-resistance genes.
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Claims 5
Claim · model-written summary
De novo assembly produced 126,402 cotton unigenes.
- Taxon
- cotton
Gossypium hirsutumt0 - Outcome
- unigenes were obtained using de novo assembly and data analysise0
- Value
- 126,402 unigenese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A total of 126,402 unigenes were obtained using de novo assembly and data analysis”
t0“cotton”
Claim · model-written summary
Of the assembled cotton unigenes, 99,712 (78.88%) were database annotated.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- annotated into the Nr, Nt, Swiss-Prot, KEGG, COG, and GO databasese0
- Value
- 99,712 (78.88%)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“99,712 (78.88%) of which were annotated into the Nr, Nt, Swiss-Prot, KEGG, COG, and GO databases.”
t0“cotton”
Claim · model-written summary
Some candidate resistance genes were upregulated soon after V. dahliae inoculation.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- V. dahliae inoculatione0
- Interactor
- V. dahliae
Verticillium dahliaee0pathogen - Gene
- candidate disease-resis-tance genese0
- Outcome
- expression patternse0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The expression patterns of 16 candidate disease-resis-tance genes showed that some genes were upregulated soon after V. dahliae inoculation”
t0“cotton”
Claim · model-written summary
Other candidate resistance genes were upregulated later after inoculation.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- V. dahliae inoculatione1
- Interactor
- V. dahliaeVerticillium dahliaee1pathogen
- Gene
- candidate disease-resis-tance genese1
- Outcome
- expression patternse1
- Direction
- upregulatede0
- Qualifier
- latere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“and others were upregulated later, which may indicate instanta-neous basal defense and lagged specific defense, respectively.”
e1“The expression patterns of 16 candidate disease-resis-tance genes showed that some genes were upregulated soon after V. dahliae inoculation”
t0“cotton”
Claim · model-written summary
Early and late upregulation may indicate instantaneous basal and lagged specific defense, respectively.
- Taxon
- cottonGossypium hirsutumt0
- Interactor
- V. dahliaeVerticillium dahliaee1pathogen
- Gene
- candidate disease-resis-tance genese1
- Outcome
- instanta-neous basal defense and lagged specific defensee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which may indicate instanta-neous basal defense and lagged specific defense, respectively.”
e1“The expression patterns of 16 candidate disease-resis-tance genes showed that some genes were upregulated soon after V. dahliae inoculation”
t0“cotton”
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Verticillium dahliae · molecular: expression · Gossypium hirsutum (cotton)Positive and negative results
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- negative
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See all 11 papers →inoculation · molecular: expression · Gossypium hirsutum (cotton)Positive and negative results
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.