Abstract
Accumulating evidence indicates that plant MYB transcription factors participate in defense against pathogen attack, but their regulatory targets and related signaling processes remain largely unknown. Here, we identified a defense-related MYB gene (GhMYB108) from upland cotton (Gossypium hirsutum) and characterized its functional mechanism. Expression of GhMYB108 in cotton plants was induced by Verticillium dahliae infection and responded to the application of defense signaling molecules, including salicylic acid, jasmonic acid, and ethylene. Knockdown of GhMYB108 expression led to increased susceptibility of cotton plants to V. dahliae, while ecotopic overexpression of GhMYB108 in Arabidopsis thaliana conferred enhanced tolerance to the pathogen. Further analysis demonstrated that GhMYB108 interacted with the calmodulin-like protein GhCML11, and the two proteins form a positive feedback loop to enhance the transcription of GhCML11 in a calcium-dependent manner. Verticillium dahliae infection stimulated Ca(2+) influx into the cytosol in cotton root cells, but this response was disrupted in both GhCML11-silenced plants and GhMYB108-silenced plants in which expression of several calcium signaling-related genes was down-regulated. Taken together, these results indicate that GhMYB108 acts as a positive regulator in defense against V. dahliae infection by interacting with GhCML11. Furthermore, the data also revealed the important roles and synergetic regulation of MYB transcription factor, Ca(2+), and calmodulin in plant immune responses.
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Claims 15
Claim · model-written summary
A defense-related GhMYB108 gene was identified from upland cotton.
- Taxon
- Gossypium hirsutum
Gossypium hirsutume0 - Gene
- GhMYB108e0
- Outcome
- defense-related MYB genee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Here, we identified a defense-related MYB gene (GhMYB108) from upland cotton (Gossypium hirsutum) and characterized its functional mechanism.”
Claim · model-written summary
Verticillium dahliae infection induced GhMYB108 expression in cotton.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- Verticillium dahliae infectione0
- Interactor
- Verticillium dahliae
Verticillium dahliaee0pathogen - Gene
- GhMYB108e0
- Outcome
- Expression of GhMYB108e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of GhMYB108 in cotton plants was induced by Verticillium dahliae infection and responded to the application of defense signaling molecules, including salicylic acid, jasmonic acid, and ethylene.”
Claim · model-written summary
Cotton GhMYB108 expression responded to salicylic acid application.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- salicylic acide0
- Gene
- GhMYB108e0
- Outcome
- Expression of GhMYB108e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of GhMYB108 in cotton plants was induced by Verticillium dahliae infection and responded to the application of defense signaling molecules, including salicylic acid, jasmonic acid, and ethylene.”
Claim · model-written summary
Cotton GhMYB108 expression responded to jasmonic acid application.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- jasmonic acide0
- Gene
- GhMYB108e0
- Outcome
- Expression of GhMYB108e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of GhMYB108 in cotton plants was induced by Verticillium dahliae infection and responded to the application of defense signaling molecules, including salicylic acid, jasmonic acid, and ethylene.”
Claim · model-written summary
Cotton GhMYB108 expression responded to ethylene application.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- ethylenee0
- Gene
- GhMYB108e0
- Outcome
- Expression of GhMYB108e0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of GhMYB108 in cotton plants was induced by Verticillium dahliae infection and responded to the application of defense signaling molecules, including salicylic acid, jasmonic acid, and ethylene.”
Claim · model-written summary
GhMYB108 knockdown increased cotton susceptibility to V. dahliae.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- Knockdown of GhMYB108 expressione0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Gene
- GhMYB108e0
- Outcome
- susceptibilitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockdown of GhMYB108 expression led to increased susceptibility of cotton plants to V. dahliae”
Claim · model-written summary
GhMYB108 overexpression enhanced Arabidopsis thaliana tolerance to V. dahliae.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- ecotopic overexpression of GhMYB108e0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Gene
- GhMYB108e0
- Outcome
- tolerancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Knockdown of GhMYB108 expression led to increased susceptibility of cotton plants to V. dahliae, while ecotopic overexpression of GhMYB108 in Arabidopsis thaliana conferred enhanced tolerance to the pathogen.”
Claim · model-written summary
GhMYB108 interacted with GhCML11.
- Taxon
- Gossypium hirsutumGossypium hirsutume1
- Gene
- GhMYB108e0
- Outcome
- interacted with the calmodulin-like protein GhCML11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Further analysis demonstrated that GhMYB108 interacted with the calmodulin-like protein GhCML11”
e1“upland cotton (Gossypium hirsutum)”
Claim · model-written summary
GhMYB108 and GhCML11 form a calcium-dependent positive feedback loop enhancing GhCML11 transcription.
- Taxon
- Gossypium hirsutumGossypium hirsutume1
- Gene
- GhMYB108e0
- Compound
- calciume0
- Outcome
- transcription of GhCML11e0
- Direction
- enhancee0
- Qualifier
- positive feedback loope0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Further analysis demonstrated that GhMYB108 interacted with the calmodulin-like protein GhCML11, and the two proteins form a positive feedback loop to enhance the transcription of GhCML11 in a calcium-dependent manner.”
e1“upland cotton (Gossypium hirsutum)”
Claim · model-written summary
Verticillium dahliae infection stimulated cytosolic calcium influx in cotton root cells.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- root cellse0
- Treatment
- Verticillium dahliae infectione0
- Interactor
- Verticillium dahliaeVerticillium dahliaee0pathogen
- Outcome
- Ca(2+) influx into the cytosole0
- Direction
- stimulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Verticillium dahliae infection stimulated Ca(2+) influx into the cytosol in cotton root cells”
Claim · model-written summary
GhCML11 silencing disrupted the infection-induced calcium influx response.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- GhCML11-silencede0
- Interactor
- Verticillium dahliaeVerticillium dahliaee0pathogen
- Gene
- GhCML11e0
- Outcome
- Ca(2+) influx into the cytosole0
- Direction
- disruptede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Verticillium dahliae infection stimulated Ca(2+) influx into the cytosol in cotton root cells, but this response was disrupted in both GhCML11-silenced plants and GhMYB108-silenced plants in which expression of several calcium signaling-related genes was down-regulated.”
Claim · model-written summary
GhMYB108 silencing disrupted the infection-induced calcium influx response.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- GhMYB108-silencede0
- Interactor
- Verticillium dahliaeVerticillium dahliaee0pathogen
- Gene
- GhMYB108e0
- Outcome
- Ca(2+) influx into the cytosole0
- Direction
- disruptede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Verticillium dahliae infection stimulated Ca(2+) influx into the cytosol in cotton root cells, but this response was disrupted in both GhCML11-silenced plants and GhMYB108-silenced plants in which expression of several calcium signaling-related genes was down-regulated.”
Claim · model-written summary
Calcium signaling-related gene expression was down-regulated in GhMYB108-silenced cotton plants.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- GhMYB108-silencede0
- Gene
- GhMYB108e0
- Outcome
- expression of several calcium signaling-related genese0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Verticillium dahliae infection stimulated Ca(2+) influx into the cytosol in cotton root cells, but this response was disrupted in both GhCML11-silenced plants and GhMYB108-silenced plants in which expression of several calcium signaling-related genes was down-regulated.”
Claim · model-written summary
GhMYB108 positively regulates defense against V. dahliae through interaction with GhCML11.
- Taxon
- Gossypium hirsutumGossypium hirsutume1
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Gene
- GhMYB108e0
- Outcome
- defense against V. dahliae infectione0
- Qualifier
- interacting with GhCML11e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Taken together, these results indicate that GhMYB108 acts as a positive regulator in defense against V. dahliae infection by interacting with GhCML11.”
e1“upland cotton (Gossypium hirsutum)”
Claim · model-written summary
MYB transcription factor, calcium and calmodulin have important synergistic regulatory roles in plant immune responses.
- Taxon
- Gossypium hirsutumGossypium hirsutume1
- Gene
- MYB transcription factore0
- Compound
- Ca(2+)e0
- Outcome
- plant immune responsese0
- Qualifier
- synergetic regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Furthermore, the data also revealed the important roles and synergetic regulation of MYB transcription factor, Ca(2+), and calmodulin in plant immune responses.”
e1“upland cotton (Gossypium hirsutum)”
The same questions in other papers
Verticillium dahliae · molecular: expression · Gossypium hirsutum (cotton)Positive and negative results
9 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
7 papers more in this group
See all 11 papers →infection · molecular: expression · Gossypium hirsutum (cotton)Positive and negative results
7 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- no effect
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.