Abstract
An effective in vitro culture system for mature nodal segments of European plum (Prunus domestica) was established. Several important aspects of regeneration from nodal explants were studied for adaptation of European plum in Bangladesh. Leaf explants were used for callus induction and ½ MS media supplemented with 1.0 mg/l indole butyric acid (IBA) + 0.5 mg/l naphthalene acetic acid (NAA) induced low efficiency callus. The nodal segments of 1.5 cm were cut from young plants, sterilized and established in vitro. The successful cultures were achieved on MS media supplemented with 2-6 mg/l kinetin (KIN); 2, 3, 5 mg/l benzyleaminopurine (BAP) and 4 mg/l KIN combination with 2 mg/l BAP. The highest number of shoot length (1.20 ±0.15 cm), highest number of shoot (3.00±0.35 cm), and highest number of leaf (14.25±1.45) were observed at KIN 4.0 mg/l. The successful multiple shoots were achieved on MS media with 2-6 mg/l KIN, 4 mg/l KIN combination with 2 mg/l BAP. This indicates that, in general, European plum species is very responsive to in vitro regeneration from nodal segment. The shoots from micro cutting were rooted in MS media supplemented with 1.0 mg/l IBA and with 0.5 mg/l BAP; ½ MS media supplemented with 0.5 mg/l IBA + 0.5mg/l NAA and 1.0 mg/l IBA + 0.5 mg/l BAP. Regenerated plantlets successfully acclimatized to grow vigorously with no apparent phenotypic aberrations. In addition, in sense of commercial value without growth hormone achieved great result. Furthermore, the antibacterial activity of extracts of European plum was evaluated against the human pathogenic bacteria Escherichia coli, Klebsiella pneumonia, Protease mirabilis, Pseudomonas aeruginosa and Staphylococcus aureus by agar disc diffusion method. Most of the human pathogens were resistant against commercial disk. But plum fruit extract showed good inhibition zone result (14.25 mm by crude extract against P. aeruginosa and 12.4 mm by dry extract against S. aureus). The positive results of screening of this plum for antibacterial activity forms primary platform for further phytochemical and pharmacological studies.
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Claims 12
Claim · model-written summary
An effective culture system for mature European plum nodal segments was established.
- Taxon
- European plum (Prunus domestica)
Prunus domesticae0 - Organ / stage
- mature nodal segmentse0
- Outcome
- effective in vitro culture systeme0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“An effective in vitro culture system for mature nodal segments of European plum (Prunus domestica) was established”
Claim · model-written summary
IBA and NAA medium induced low efficiency callus from leaf explants.
- Taxon
- Prunus domestica L.Prunus domesticat0
- Organ / stage
- Leaf explantse0
- Treatment
- ½ MS media supplemented with 1.0 mg/l indole butyric acid (IBA) + 0.5 mg/l naphthalene acetic acid (NAA)e0
- Outcome
- low efficiency calluse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Prunus domestica L.”
e0“Leaf explants were used for callus induction and ½ MS media supplemented with 1.0 mg/l indole butyric acid (IBA) + 0.5 mg/l naphthalene acetic acid (NAA) induced low efficiency callus”
Claim · model-written summary
The listed KIN and BAP media yielded successful nodal cultures.
- Taxon
- Prunus domestica L.Prunus domesticat0
- Treatment
- MS media supplemented with 2-6 mg/l kinetin (KIN); 2, 3, 5 mg/l benzyleaminopurine (BAP) and 4 mg/l KIN combination with 2 mg/l BAPe0
- Outcome
- successful culturese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Prunus domestica L.”
e0“The successful cultures were achieved on MS media supplemented with 2-6 mg/l kinetin (KIN); 2, 3, 5 mg/l benzyleaminopurine (BAP) and 4 mg/l KIN combination with 2 mg/l BAP”
Claim · model-written summary
KIN at 4.0 mg/l gave the greatest shoot length, 1.20 ±0.15 cm.
- Taxon
- Prunus domestica L.Prunus domesticat0
- Treatment
- KIN 4.0 mg/le0
- Outcome
- shoot lengthe0
- Direction
- higheste0
- Value
- 1.20 ±0.15 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Prunus domestica L.”
e0“highest number of shoot length (1.20 ±0.15 cm), highest number of shoot (3.00±0.35 cm), and highest number of leaf (14.25±1.45) were observed at KIN 4.0 mg/l”
Claim · model-written summary
KIN at 4.0 mg/l gave the greatest shoot number, 3.00±0.35 cm as reported.
- Taxon
- Prunus domestica L.Prunus domesticat0
- Treatment
- KIN 4.0 mg/le0
- Outcome
- number of shoote0
- Direction
- higheste0
- Value
- 3.00±0.35 cme0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Prunus domestica L.”
e0“highest number of shoot (3.00±0.35 cm), and highest number of leaf (14.25±1.45) were observed at KIN 4.0 mg/l”
Claim · model-written summary
KIN at 4.0 mg/l gave the greatest leaf number, 14.25±1.45.
- Taxon
- Prunus domestica L.Prunus domesticat0
- Treatment
- KIN 4.0 mg/le0
- Outcome
- number of leafe0
- Direction
- higheste0
- Value
- 14.25±1.45e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Prunus domestica L.”
e0“highest number of leaf (14.25±1.45) were observed at KIN 4.0 mg/l”
Claim · model-written summary
Multiple shoots were achieved on KIN medium or KIN plus BAP medium.
- Taxon
- Prunus domestica L.Prunus domesticat0
- Treatment
- MS media with 2-6 mg/l KIN, 4 mg/l KIN combination with 2 mg/l BAPe0
- Outcome
- successful multiple shootse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Prunus domestica L.”
e0“The successful multiple shoots were achieved on MS media with 2-6 mg/l KIN, 4 mg/l KIN combination with 2 mg/l BAP”
Claim · model-written summary
Microcutting shoots rooted on the reported IBA and BAP or NAA media.
- Taxon
- Prunus domestica L.Prunus domesticat0
- Outcome
- shoots from micro cutting were rootede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Prunus domestica L.”
e0“The shoots from micro cutting were rooted in MS media supplemented with 1.0 mg/l IBA and with 0.5 mg/l BAP; ½ MS media supplemented with 0.5 mg/l IBA + 0.5mg/l NAA and 1.0 mg/l IBA + 0.5 mg/l BAP”
Claim · model-written summary
Regenerated plum plantlets acclimatized with vigorous growth and no apparent aberrations.
- Taxon
- Prunus domestica L.Prunus domesticat0
- Outcome
- successfully acclimatized to grow vigorously with no apparent phenotypic aberrationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Prunus domestica L.”
e0“Regenerated plantlets successfully acclimatized to grow vigorously with no apparent phenotypic aberrations”
Claim · model-written summary
Hormone-free commercial culture produced a favorable result.
- Taxon
- Prunus domestica L.Prunus domesticat0
- Treatment
- without growth hormonee0
- Outcome
- great resulte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Prunus domestica L.”
e0“in sense of commercial value without growth hormone achieved great result”
Claim · model-written summary
Crude plum fruit extract inhibited P. aeruginosa with a 14.25 mm zone.
- Taxon
- plumPrunus domesticae0
- Treatment
- crude extracte0
- Outcome
- inhibition zone resulte0
- Value
- 14.25 mme0
- Qualifier
- P. aeruginosae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plum fruit extract showed good inhibition zone result (14.25 mm by crude extract against P. aeruginosa and 12.4 mm by dry extract against S. aureus)”
Claim · model-written summary
Dry plum fruit extract inhibited S. aureus with a 12.4 mm zone.
- Taxon
- plumPrunus domesticae0
- Treatment
- dry extracte0
- Outcome
- inhibition zone resulte0
- Value
- 12.4 mme0
- Qualifier
- S. aureuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plum fruit extract showed good inhibition zone result (14.25 mm by crude extract against P. aeruginosa and 12.4 mm by dry extract against S. aureus)”
The same questions in other papers
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in vitro · growth · Prunus domestica (plum)Positive and negative results
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.