Paper · 2006 · Biologia Plantarum

Improved plant regeneration in Capsicum annuum L. from nodal segments

N. Ahmad; I. Siddique; M. Anis

6
claims
12
verbatim quotes
54
citations
2
research areas
✓
Open access

Abstract

Multiple shoots were induced by culturing nodal explants excised from 1-month-old aseptic seedlings of red pepper (Capsicum annuum L. cv. Pusa Jwala) on Murashige and Skoog (MS) medium supplemented with (0.1-10 μM) thidiazuron (TDZ). The rate of multiple shoot induction per explant was maximum (14.4 ± 0.06) on MS medium supplemented with 1.0 μM TDZ. Regenerated shoots were elongated well on growth regulator free MS medium. Adventitious roots were induced two weeks after transfer of elongated shoots to MS medium supplemented with auxins (IAA, IBA or NAA) in different concentrations. Optimum root formation frequency was obtained in medium containing 1.0 μM IBA. Ex-vitro rooting was also achieved by pulse treatment with 300 μM IBA for 10 min. Rooted shoots were transplanted in plastic pots containing garden soil (with 90 % survival rate), where they grew well and attained maturity. Regenerated plants were phenotypically and cytologically normal.

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Claims 6

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Multiple shoot induction per explant of Capsicum annuum nodal explants was maximum (14.4 ± 0.06) on MS medium with 1.0 μM TDZ.

Taxon
Capsicum annuum L.Capsicum annuumt0
Treatment
1.0 μM TDZe0
Outcome
rate of multiple shoot induction per explante0
Direction
maximume0
Value
14.4 ± 0.06e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The rate of multiple shoot induction per explant was maximum (14.4 ± 0.06) on MS medium supplemented with 1.0 μM TDZ”

✓ located verbatim in the source · abstract, characters 234–350 · show in text ↑
t0

“Capsicum annuum L.”

✓ located verbatim in the source · title, characters 31–49 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Regenerated Capsicum annuum shoots elongated well on growth regulator free MS medium.

Taxon
Capsicum annuum L.Capsicum annuumt0
Treatment
growth regulator free MS mediume0
Outcome
elongated welle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Regenerated shoots were elongated well on growth regulator free MS medium”

✓ located verbatim in the source · abstract, characters 352–425 · show in text ↑
t0

“Capsicum annuum L.”

✓ located verbatim in the source · title, characters 31–49 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Optimum root formation frequency of Capsicum annuum shoots was obtained in medium containing 1.0 μM IBA.

Taxon
Capsicum annuum L.Capsicum annuumt0
Treatment
1.0 μM IBAe0
Outcome
root formation frequencye0
Direction
Optimume0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Optimum root formation frequency was obtained in medium containing 1.0 μM IBA”

✓ located verbatim in the source · abstract, characters 589–666 · show in text ↑
t0

“Capsicum annuum L.”

✓ located verbatim in the source · title, characters 31–49 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Ex-vitro rooting of Capsicum annuum shoots was achieved by pulse treatment with 300 μM IBA for 10 min.

Taxon
Capsicum annuum L.Capsicum annuumt0
Treatment
pulse treatment with 300 μM IBA for 10 mine0
Outcome
Ex-vitro rootinge0

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Ex-vitro rooting was also achieved by pulse treatment with 300 μM IBA for 10 min”

✓ located verbatim in the source · abstract, characters 668–748 · show in text ↑
t0

“Capsicum annuum L.”

✓ located verbatim in the source · title, characters 31–49 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Rooted Capsicum annuum shoots transplanted into plastic pots with garden soil had a 90 % survival rate.

Taxon
Capsicum annuum L.Capsicum annuumt0
Outcome
survival ratee0
Value
90 %e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Rooted shoots were transplanted in plastic pots containing garden soil (with 90 % survival rate)”

✓ located verbatim in the source · abstract, characters 750–846 · show in text ↑
t0

“Capsicum annuum L.”

✓ located verbatim in the source · title, characters 31–49 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Regenerated Capsicum annuum plants were phenotypically and cytologically normal.

Taxon
Capsicum annuum L.Capsicum annuumt0
Outcome
phenotypically and cytologically normale0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Capsicum annuum
Capsicum annuum · TaxonBilljones94 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Regenerated plants were phenotypically and cytologically normal”

✓ located verbatim in the source · abstract, characters 892–955 · show in text ↑
t0

“Capsicum annuum L.”

✓ located verbatim in the source · title, characters 31–49 · show in text ↑

Thidiazuron · growth · Capsicum annuum (sweet pepper)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

indole-3-butyric acid · growth · Capsicum annuum (sweet pepper)Results agree

6 papers: 6 positive, 0 negative or no effect

5 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.