Paper · 2013

Suppression of Bacterial Wilt Disease of Tomato Plants Using Some Bacterial Strains

Abeer H. Makhlouf

11
claims
21
verbatim quotes
3
citations
3
research areas
—
Not open access

Abstract

Six isolates of Ralstoniasolanacearum were isolated from naturally wilted roots of tomato plants grown in Minufiya governorate. All isolates were pathogenic to tomato plants and produced typical symptoms of wilt. Isolate No. 6 exh ibited the highest virulence followed by isolates No. 4. Characterization of strains of Ralstoniasolanacearum , were performed based on pathogenicity, Biochemical and physiological tests. Pseudomonas aeruginosa , Pseudomonas putida , Bacillus cereus , Pseudomo nas syrinagae , Pseudomonas stutzeri , and Bacillus thuringiensis were isolated from tomato rhizosphere as biocontrol agents and tested against Ralstonia solanacearum (R. solanacearum) in vitro and in vivo . All the bio - control agents test ed reduced the bacterial wilt disease to various degrees. the physiological and biological characters of six isolates revealed similar characters . Under greenhouse conditions, Pseudomonas aeruginosa (P. aeruginosa ) and Bacillus thuringiensis exhibited the h ighest disease reduction of tomato bacterial wilt disease (89%, 86% respectively) followed by P. putida, Bacillus cereus, and P. stutzeri (70%, 68% and 65%) while P. syrinagae showed the lowest disease reduction (61%). Two bacterial strains which showed h ighly antagonistic activity towards tomato bacterial wilt were identified using specific polymerase chain reaction (PCR) of 16S rDNA gene. The 16SrDNA sequence analysis showed that the 1 st strain belongs to the genus Pseudomonas , with closest similarity to Pseudomonasaeruginosa (100% similarity ) . The 2 nd strain identified

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Claims 11

Observationobservedoriginal resultmeasured

Claim · model-written summary

Six isolates from wilted tomato roots were pathogenic and caused typical wilt.

Taxon
tomato plantsSolanum lycopersicume0
Interactor
Ralstoniasolanacearume0pathogen
Outcome
pathogenic to tomato plants and produced typical symptoms of wilte0
Setting
Minufiya governoratee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Six isolates of Ralstoniasolanacearum were isolated from naturally wilted roots of tomato plants grown in Minufiya governorate. All isolates were pathogenic to tomato plants and produced typical symptoms of wilt.”

✓ located verbatim in the source · abstract, characters 0–212 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Isolate No. 6 showed the highest virulence, followed by isolate No. 4.

Taxon
Tomato PlantsSolanum lycopersicumt0
Material
Isolate No. 6e0
Outcome
virulencee0
Direction
higheste0
Comparator
isolates No. 4e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Isolate No. 6 exh ibited the highest virulence followed by isolates No. 4.”

✓ located verbatim in the source · abstract, characters 213–287 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 41–54 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

All tested biocontrol agents reduced tomato bacterial wilt to varying degrees.

Taxon
Tomato PlantsSolanum lycopersicumt0
Treatment
bio - control agentse0
Outcome
bacterial wilt diseasee0
Direction
reducede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“All the bio - control agents test ed reduced the bacterial wilt disease to various degrees.”

✓ located verbatim in the source · abstract, characters 700–791 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 41–54 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Pseudomonas aeruginosa reduced tomato bacterial wilt by 89%.

Taxon
Tomato PlantsSolanum lycopersicumt0
Treatment
Pseudomonas aeruginosae0
Interactor
Pseudomonas aeruginosaPseudomonas aeruginosae0biocontrol_agent
Outcome
tomato bacterial wilt diseasee0
Direction
reductione0
Value
89%e0
Setting
greenhouse conditionse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Pseudomonas aeruginosa
Pseudomonas aeruginosa · InteractorCDC · Public domain · Wikimedia Commons ↗

Verbatim

e0

“Under greenhouse conditions, Pseudomonas aeruginosa (P. aeruginosa ) and Bacillus thuringiensis exhibited the h ighest disease reduction of tomato bacterial wilt disease (89%, 86% respectively) followed by P. putida, Bacillus cereus, and P. stutzeri (70%, 68% and 65%) while P. syrinagae showed the lowest disease reduction (61%).”

✓ located verbatim in the source · abstract, characters 882–1212 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 41–54 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Bacillus thuringiensis reduced tomato bacterial wilt by 86%.

Taxon
Tomato PlantsSolanum lycopersicumt0
Treatment
Bacillus thuringiensise0
Interactor
Bacillus thuringiensisBacillus thuringiensise0biocontrol_agent
Outcome
tomato bacterial wilt diseasee0
Direction
reductione0
Value
86%e0
Setting
greenhouse conditionse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Bacillus thuringiensis
Bacillus thuringiensis · InteractorSam LaRussa · CC BY 4.0 · iNaturalist ↗

Verbatim

e0

“Under greenhouse conditions, Pseudomonas aeruginosa (P. aeruginosa ) and Bacillus thuringiensis exhibited the h ighest disease reduction of tomato bacterial wilt disease (89%, 86% respectively) followed by P. putida, Bacillus cereus, and P. stutzeri (70%, 68% and 65%) while P. syrinagae showed the lowest disease reduction (61%).”

✓ located verbatim in the source · abstract, characters 882–1212 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 41–54 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

P. putida reduced tomato bacterial wilt by 70%.

Taxon
Tomato PlantsSolanum lycopersicumt0
Treatment
P. putidae0
Interactor
P. putidaPseudomonas putidae0biocontrol_agent
Outcome
tomato bacterial wilt diseasee0
Direction
reductione0
Value
70%e0
Setting
greenhouse conditionse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Pseudomonas putida
Pseudomonas putida · InteractorMroghair · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Under greenhouse conditions, Pseudomonas aeruginosa (P. aeruginosa ) and Bacillus thuringiensis exhibited the h ighest disease reduction of tomato bacterial wilt disease (89%, 86% respectively) followed by P. putida, Bacillus cereus, and P. stutzeri (70%, 68% and 65%) while P. syrinagae showed the lowest disease reduction (61%).”

✓ located verbatim in the source · abstract, characters 882–1212 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 41–54 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Bacillus cereus reduced tomato bacterial wilt by 68%.

Taxon
Tomato PlantsSolanum lycopersicumt0
Treatment
Bacillus cereuse0
Interactor
Bacillus cereusBacillus cereuse0biocontrol_agent
Outcome
tomato bacterial wilt diseasee0
Direction
reductione0
Value
68%e0
Setting
greenhouse conditionse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗
Bacillus cereus
Bacillus cereus · InteractorMogana Das Murtey and Patchamuthu Ramasamy · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Under greenhouse conditions, Pseudomonas aeruginosa (P. aeruginosa ) and Bacillus thuringiensis exhibited the h ighest disease reduction of tomato bacterial wilt disease (89%, 86% respectively) followed by P. putida, Bacillus cereus, and P. stutzeri (70%, 68% and 65%) while P. syrinagae showed the lowest disease reduction (61%).”

✓ located verbatim in the source · abstract, characters 882–1212 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 41–54 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

P. stutzeri reduced tomato bacterial wilt by 65%.

Taxon
Tomato PlantsSolanum lycopersicumt0
Treatment
P. stutzerie0
Interactor
P. stutzerie0biocontrol_agent
Outcome
tomato bacterial wilt diseasee0
Direction
reductione0
Value
65%e0
Setting
greenhouse conditionse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Under greenhouse conditions, Pseudomonas aeruginosa (P. aeruginosa ) and Bacillus thuringiensis exhibited the h ighest disease reduction of tomato bacterial wilt disease (89%, 86% respectively) followed by P. putida, Bacillus cereus, and P. stutzeri (70%, 68% and 65%) while P. syrinagae showed the lowest disease reduction (61%).”

✓ located verbatim in the source · abstract, characters 882–1212 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 41–54 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

P. syrinagae reduced tomato bacterial wilt by 61%.

Taxon
Tomato PlantsSolanum lycopersicumt0
Treatment
P. syrinagaee0
Interactor
P. syrinagaee0biocontrol_agent
Outcome
tomato bacterial wilt diseasee0
Direction
reductione0
Value
61%e0
Setting
greenhouse conditionse0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Under greenhouse conditions, Pseudomonas aeruginosa (P. aeruginosa ) and Bacillus thuringiensis exhibited the h ighest disease reduction of tomato bacterial wilt disease (89%, 86% respectively) followed by P. putida, Bacillus cereus, and P. stutzeri (70%, 68% and 65%) while P. syrinagae showed the lowest disease reduction (61%).”

✓ located verbatim in the source · abstract, characters 882–1212 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 41–54 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Six pathogen isolates had similar physiological and biological characters.

Taxon
Tomato PlantsSolanum lycopersicumt0
Outcome
similar characterse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“the physiological and biological characters of six isolates revealed similar characters”

✓ located verbatim in the source · abstract, characters 792–879 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 41–54 · show in text ↑
Method / resourceobservedoriginal resultmeasured

Claim · model-written summary

One antagonistic strain matched Pseudomonasaeruginosa at 100% sequence similarity.

Taxon
Tomato PlantsSolanum lycopersicumt0
Outcome
closest similarity to Pseudomonasaeruginosae0
Value
100% similaritye0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Solanum lycopersicum
Solanum lycopersicum · TaxonKod B · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“The 16SrDNA sequence analysis showed that the 1 st strain belongs to the genus Pseudomonas , with closest similarity to Pseudomonasaeruginosa (100% similarity )”

✓ located verbatim in the source · abstract, characters 1389–1549 · show in text ↑
t0

“Tomato Plants”

✓ located verbatim in the source · title, characters 41–54 · show in text ↑

Pseudomonas putida · disease · Solanum lycopersicum (tomato)Positive and negative results

7 papers: 6 positive, 1 negative or no effect

5 papers in the same direction as this paper · 1 in the opposite direction

  1. positive
  2. no effect
  3. positive

3 papers more in this group

See all 7 papers →

Pseudomonas aeruginosa · disease · Solanum lycopersicum (tomato)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

  1. positive
  2. positive
See all 3 papers →

Bacillus thuringiensis · disease · Solanum lycopersicum (tomato)Results agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

Bacillus cereus · disease · Solanum lycopersicum (tomato)Results agree

6 papers: 6 positive, 0 negative or no effect

5 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

2 papers more in this group

See all 6 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.