Abstract
Pongsu Seribu 2 (PS2) is a land race traditional rice variety grown in Malaysia having broad spectrum resistance to blast disease caused by Magnaporthe oryzae. The information on genetic basis of blast resistance in this cultivar is still lacking. In order to ensure the resistance of Pongsu Seribu 2, virulent pathotype of M. oryzae P7.2 was inoculated on Pongsu Seribu 2 and MR219 (susceptible used as control) where Pongsu Seribu 2 was found strongly resistance and MR219 susceptible. To determine the mechanism of resistance in PS2 cultivar, cloning and characterization of major blast resistance genes, Pi-b and Pi-kh were carried out. Primers covering the Pi-b and Pi-kh nucleotide sequence available in NCBI database were designed that successfully amplified the PCR PCR product. The amplified product was ligated with PGEM-T easy vector and cloned the blast resistance genes fragment into competent cell. The results revealed that nucleotide sequence contain an open reading frame (ORF) and the same is also highly conserved in nature. Deduced amino acid sequence indicates that Pi-b contains zinc finger-containing protein domain and Pi-kh have Leucine rich repeat domain. The translated nucleotide sequence into amino acid produces significant homology average 76.8% with Pi-kh and 93.4% with Pi-b blast resistance genes present in different cultivars of rice. The amino acid sequence of both genes also showed homology with NBS-LRR (Nucleotide binding site-Leucine rich repeat), proteins and BAC clones covering the chromosome 2 and 12 of rice with different cultivars. This study indicates that Pongsu Seribu 2 contain at least two dominant genes, Pi-b and Pi-kh involved for providing resistance against M. oryzae pathotype P7.2.
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Claims 9
Claim · model-written summary
After inoculation with the virulent Magnaporthe oryzae pathotype P7.2, the rice landrace Pongsu Seribu 2 was strongly resistant compared with the susceptible control MR219.
- Taxon
- rice
Oryza sativat0 - Material
- Pongsu Seribu 2e0
- Treatment
- virulent pathotype of M. oryzae P7.2e0
- Interactor
- M. oryzae
Pyricularia oryzaee0pathogen - Outcome
- strongly resistancee0
- Comparator
- MR219e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Malaysian rice variety Pongsu Seribu 2”
e0“virulent pathotype of M. oryzae P7.2 was inoculated on Pongsu Seribu 2 and MR219 (susceptible used as control) where Pongsu Seribu 2 was found strongly resistance and MR219 susceptible”
Claim · model-written summary
The rice cultivar MR219, used as susceptible control, was susceptible to Magnaporthe oryzae pathotype P7.2.
- Taxon
- riceOryza sativat0
- Material
- MR219e0
- Treatment
- virulent pathotype of M. oryzae P7.2e0
- Interactor
- M. oryzaePyricularia oryzaee0pathogen
- Outcome
- susceptiblee0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Malaysian rice variety Pongsu Seribu 2”
e0“virulent pathotype of M. oryzae P7.2 was inoculated on Pongsu Seribu 2 and MR219 (susceptible used as control) where Pongsu Seribu 2 was found strongly resistance and MR219 susceptible”
Claim · model-written summary
The cloned Pi-b and Pi-kh nucleotide sequences contain an open reading frame that is highly conserved.
- Taxon
- riceOryza sativat0
- Material
- Pongsu Seribu 2t0
- Gene
- Pi-b and Pi-kht1
- Outcome
- open reading framee0
- Qualifier
- highly conserved in naturee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice variety Pongsu Seribu 2”
t1“genes Pi-b and Pi-kh”
e0“nucleotide sequence contain an open reading frame (ORF) and the same is also highly conserved in nature”
Claim · model-written summary
The deduced amino acid sequence indicates that Pi-b contains a zinc finger-containing protein domain.
- Taxon
- riceOryza sativat0
- Material
- Pongsu Seribu 2t0
- Gene
- Pi-be0
- Outcome
- zinc finger-containing protein domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice variety Pongsu Seribu 2”
e0“Pi-b contains zinc finger-containing protein domain”
Claim · model-written summary
The deduced amino acid sequence indicates that Pi-kh has a leucine-rich repeat domain.
- Taxon
- riceOryza sativat0
- Material
- Pongsu Seribu 2t0
- Gene
- Pi-khe0
- Outcome
- Leucine rich repeat domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice variety Pongsu Seribu 2”
e0“Pi-kh have Leucine rich repeat domain”
Claim · model-written summary
The translated amino acid sequence showed significant average homology of 76.8% with Pi-kh blast resistance genes of other cultivars.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice variety Pongsu Seribu 2”
e0“significant homology average 76.8% with Pi-kh”
Claim · model-written summary
The translated amino acid sequence showed significant average homology of 93.4% with Pi-b blast resistance genes of other cultivars.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice variety Pongsu Seribu 2”
e0“significant homology average 76.8% with Pi-kh and 93.4% with Pi-b”
Claim · model-written summary
The amino acid sequences of both genes showed homology with NBS-LRR proteins and with BAC clones covering rice chromosomes 2 and 12 in different cultivars.
- Taxon
- riceOryza sativae0
- Gene
- Pi-b and Pi-kht1
- Outcome
- homology with NBS-LRRe0
- Qualifier
- BAC clones covering the chromosome 2 and 12e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“genes Pi-b and Pi-kh”
e0“The amino acid sequence of both genes also showed homology with NBS-LRR (Nucleotide binding site-Leucine rich repeat), proteins and BAC clones covering the chromosome 2 and 12 of rice with different cultivars”
Claim · model-written summary
Pongsu Seribu 2 contains at least two dominant genes, Pi-b and Pi-kh, involved in resistance against Magnaporthe oryzae pathotype P7.2.
- Taxon
- riceOryza sativat0
- Material
- Pongsu Seribu 2e0
- Interactor
- M. oryzaePyricularia oryzaee0pathogen
- Gene
- Pi-b and Pi-khe0
- Outcome
- resistancee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“rice variety Pongsu Seribu 2”
e0“Pongsu Seribu 2 contain at least two dominant genes, Pi-b and Pi-kh involved for providing resistance against M. oryzae pathotype P7.2”
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