Abstract
[1'-(14)C, (13)C(6)]Indole-3-acetic acid was infiltrated into immature pericarp discs from fruits of tomato (Lycopersicon esculentum Mill., cv Moneymaker). After a 24-h incubation period the discs were extracted with methanol and the partially purified extract was analyzed by reversed-phase high-performance liquid chromatography-radiocounting. Five metabolite peaks (1-5) were detected and subsequently analyzed by combined high-performance liquid chromatography-frit-fast atom bombardment-mass spectrometry. The metabolite 4 fraction was found to contain [(13)C(6)]-indole-3-acetylaspartic acid, and analysis of metabolite 5 identified [(13)C(6)]indole-3-acetyl-beta-d-glucose. The other metabolites could not be identified, but alkaline hydrolysis studies and gel permeation chromatography indicated that metabolites 1 and 3 were both amide conjugates with a molecular weight of approximately 600. Studies with radiolabeled indole-3-acetic acid, indole-3-acetylaspartic acid, and indole-3-acetyl-beta-d-glucose demonstrated that in immature pericarp indole-3-acetic acid is deactivated primarily via metabolism to indole-3-acetylaspartic acid, which is further converted to metabolites 1, 2, and 3. In mature, pink pericarp discs, indole-3-acetic acid is converted more extensively to its glucosyl conjugate. Conjugation of indole-3-acetic acid to indole-3-acetylaspartic acid appears to be dependent upon protein synthesis because it is inhibited by cycloheximide. In contrast, cycloheximide has little effect on the further conversion of indole-3-acetylaspartic acid to metabolites 1, 2, and 3.
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Claims 9
Claim · model-written summary
Metabolite 4 in immature tomato pericarp discs contained labelled indole-3-acetylaspartic acid
- Taxon
- tomato
Solanum lycopersicume0 - Outcome
- [(13)C(6)]-indole-3-acetylaspartic acide1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“infiltrated into immature pericarp discs from fruits of tomato (Lycopersicon esculentum Mill., cv Moneymaker)”
e1“The metabolite 4 fraction was found to contain [(13)C(6)]-indole-3-acetylaspartic acid”
Claim · model-written summary
Metabolite 5 in tomato pericarp discs was identified as labelled indole-3-acetyl-beta-d-glucose
- Taxon
- tomatoSolanum lycopersicume0
- Outcome
- [(13)C(6)]indole-3-acetyl-beta-d-glucosee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“infiltrated into immature pericarp discs from fruits of tomato (Lycopersicon esculentum Mill., cv Moneymaker)”
e2“analysis of metabolite 5 identified [(13)C(6)]indole-3-acetyl-beta-d-glucose”
Claim · model-written summary
The other tomato pericarp metabolites could not be identified
- Taxon
- tomatoSolanum lycopersicume0
- Outcome
- other metabolitese3
- Qualifier
- could not be identifiede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“infiltrated into immature pericarp discs from fruits of tomato (Lycopersicon esculentum Mill., cv Moneymaker)”
e3“The other metabolites could not be identified”
Claim · model-written summary
Metabolites 1 and 3 were amide conjugates with a molecular weight of approximately 600
- Taxon
- tomatoSolanum lycopersicume0
- Outcome
- metabolites 1 and 3e4
- Qualifier
- molecular weight of approximately 600e4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“infiltrated into immature pericarp discs from fruits of tomato (Lycopersicon esculentum Mill., cv Moneymaker)”
e4“metabolites 1 and 3 were both amide conjugates with a molecular weight of approximately 600”
Claim · model-written summary
In immature pericarp, indole-3-acetic acid is deactivated primarily via metabolism to indole-3-acetylaspartic acid
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- immature pericarpe5
- Outcome
- deactivated primarily via metabolism to indole-3-acetylaspartic acide5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“infiltrated into immature pericarp discs from fruits of tomato (Lycopersicon esculentum Mill., cv Moneymaker)”
e5“in immature pericarp indole-3-acetic acid is deactivated primarily via metabolism to indole-3-acetylaspartic acid”
Claim · model-written summary
Indole-3-acetylaspartic acid is further converted to metabolites 1, 2, and 3 in tomato pericarp
- Taxon
- tomatoSolanum lycopersicume0
- Outcome
- further converted to metabolites 1, 2, and 3e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“infiltrated into immature pericarp discs from fruits of tomato (Lycopersicon esculentum Mill., cv Moneymaker)”
e6“which is further converted to metabolites 1, 2, and 3”
Claim · model-written summary
In mature pink pericarp discs, indole-3-acetic acid is converted more extensively to its glucosyl conjugate than in immature discs
- Taxon
- tomatoSolanum lycopersicume0
- Organ / stage
- mature, pink pericarp discse7
- Outcome
- to its glucosyl conjugatee7
- Direction
- more extensivelye7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“infiltrated into immature pericarp discs from fruits of tomato (Lycopersicon esculentum Mill., cv Moneymaker)”
e7“In mature, pink pericarp discs, indole-3-acetic acid is converted more extensively to its glucosyl conjugate”
Claim · model-written summary
Cycloheximide inhibited the conjugation of indole-3-acetic acid to indole-3-acetylaspartic acid in tomato pericarp
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- cycloheximidee1
- Outcome
- Conjugation of indole-3-acetic acid to indole-3-acetylaspartic acide1
- Direction
- inhibitede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“infiltrated into immature pericarp discs from fruits of tomato (Lycopersicon esculentum Mill., cv Moneymaker)”
e1“Conjugation of indole-3-acetic acid to indole-3-acetylaspartic acid appears to be dependent upon protein synthesis because it is inhibited by cycloheximide”
Claim · model-written summary
Cycloheximide had little effect on the further conversion of indole-3-acetylaspartic acid to metabolites 1, 2, and 3
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- cycloheximidee1
- Outcome
- further conversion of indole-3-acetylaspartic acid to metabolites 1, 2, and 3e1
- Direction
- no_changeinferrede1
- Qualifier
- little effecte1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“infiltrated into immature pericarp discs from fruits of tomato (Lycopersicon esculentum Mill., cv Moneymaker)”
e1“In contrast, cycloheximide has little effect on the further conversion of indole-3-acetylaspartic acid to metabolites 1, 2, and 3”
The same questions in other papers
cycloheximide · molecular: metabolites · Solanum lycopersicum (tomato)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.