Abstract
The POLARIS (PLS) gene of Arabidopsis was identified as a promoter trap transgenic line, showing beta-glucuronidase fusion gene expression predominantly in the embryonic and seedling root, with low expression in aerial parts. Cloning of the PLS locus revealed that the promoter trap T-DNA had inserted into a short open reading frame (ORF). Rapid amplification of cDNA ends PCR, RNA gel blot analysis, and RNase protection assays showed that the PLS ORF is located within a short ( approximately 500 nucleotides) auxin-inducible transcript and encodes a predicted polypeptide of 36 amino acid residues. pls mutants exhibit a short-root phenotype and reduced vascularization of leaves. pls roots are hyperresponsive to exogenous cytokinins and show increased expression of the cytokinin-inducible gene ARR5/IBC6 compared with the wild type. pls seedlings also are less responsive to the growth-inhibitory effects of exogenous auxin and show reduced expression of the auxin-inducible gene IAA1 compared with the wild type. The PLS peptide-encoding region of the cDNA partially complements the pls mutation and requires the PLS ORF ATG for activity, demonstrating the functionality of the peptide-encoding ORF. Ectopic expression of the PLS ORF reduces root growth inhibition by exogenous cytokinins and increases leaf vascularization. We propose that PLS is required for correct auxin-cytokinin homeostasis to modulate root growth and leaf vascular patterning.
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Claims 13
Claim · model-written summary
pls mutants show a short-root phenotype.
- Taxon
- Arabidopsis
Arabidopsist0 - Material
- pls mutantse1
- Gene
- PLSe0
- Outcome
- short-root phenotypee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene of Arabidopsis”
e0“The POLARIS (PLS) gene of Arabidopsis”
e1“pls mutants exhibit a short-root phenotype and reduced vascularization of leaves”
Claim · model-written summary
pls mutants show reduced leaf vascularization.
- Taxon
- ArabidopsisArabidopsist0
- Material
- pls mutantse1
- Outcome
- vascularization of leavese1
- Direction
- reducede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene of Arabidopsis”
e1“pls mutants exhibit a short-root phenotype and reduced vascularization of leaves”
Claim · model-written summary
The PLS ORF encodes a predicted polypeptide of 36 amino acid residues.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- PLSe0
- Outcome
- predicted polypeptidee2
- Value
- 36 amino acid residuese2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene of Arabidopsis”
e0“The POLARIS (PLS) gene of Arabidopsis”
e2“encodes a predicted polypeptide of 36 amino acid residues”
Claim · model-written summary
The PLS ORF lies within a short auxin-inducible transcript of about 500 nucleotides.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- PLS ORFe3
- Outcome
- transcripte3
- Value
- approximately 500 nucleotidese3
- Qualifier
- auxin-induciblee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene of Arabidopsis”
e3“the PLS ORF is located within a short ( approximately 500 nucleotides) auxin-inducible transcript”
Claim · model-written summary
pls roots are hyperresponsive to exogenous cytokinins.
- Taxon
- ArabidopsisArabidopsist0
- Material
- pls rootse4
- Treatment
- exogenous cytokininse4
- Outcome
- hyperresponsive to exogenous cytokininse4
- Direction
- hyperresponsivee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene of Arabidopsis”
e4“pls roots are hyperresponsive to exogenous cytokinins”
Claim · model-written summary
pls roots show increased expression of the cytokinin-inducible gene ARR5/IBC6 compared with the wild type.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- ARR5/IBC6e5
- Outcome
- expression of the cytokinin-inducible genee5
- Direction
- increasede5
- Comparator
- wild typee5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene of Arabidopsis”
e5“show increased expression of the cytokinin-inducible gene ARR5/IBC6 compared with the wild type”
Claim · model-written summary
pls seedlings are less responsive to the growth-inhibitory effects of exogenous auxin.
- Taxon
- ArabidopsisArabidopsist0
- Material
- pls seedlingse6
- Treatment
- exogenous auxine6
- Outcome
- growth-inhibitory effectse6
- Direction
- less responsivee6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene of Arabidopsis”
e6“pls seedlings also are less responsive to the growth-inhibitory effects of exogenous auxin”
Claim · model-written summary
pls seedlings show reduced expression of the auxin-inducible gene IAA1 compared with the wild type.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- IAA1e7
- Outcome
- expression of the auxin-inducible genee7
- Direction
- reducede7
- Comparator
- wild typee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene of Arabidopsis”
e7“show reduced expression of the auxin-inducible gene IAA1 compared with the wild type”
Claim · model-written summary
The PLS peptide-encoding region of the cDNA partially complements the pls mutation.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- PLS peptide-encoding region of the cDNAe4
- Gene
- PLSe4
- Outcome
- complements the pls mutatione4
- Direction
- partially complementse4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene of Arabidopsis”
e4“The PLS peptide-encoding region of the cDNA partially complements the pls mutation”
Claim · model-written summary
The PLS ORF ATG is required for the activity of the peptide-encoding ORF.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- PLS ORF ATGe4
- Outcome
- activitye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene of Arabidopsis”
e4“requires the PLS ORF ATG for activity”
Claim · model-written summary
Ectopic expression of the PLS ORF reduces root growth inhibition by exogenous cytokinins.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Ectopic expression of the PLS ORFe4
- Outcome
- root growth inhibition by exogenous cytokininse4
- Direction
- reducese4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene of Arabidopsis”
e4“Ectopic expression of the PLS ORF reduces root growth inhibition by exogenous cytokinins and increases leaf vascularization”
Claim · model-written summary
Ectopic expression of the PLS ORF increases leaf vascularization.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Ectopic expression of the PLS ORFe4
- Outcome
- leaf vascularizatione4
- Direction
- increasese4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene of Arabidopsis”
e4“Ectopic expression of the PLS ORF reduces root growth inhibition by exogenous cytokinins and increases leaf vascularization”
Claim · model-written summary
PLS is proposed to be required for auxin-cytokinin homeostasis that modulates root growth and leaf vascular patterning.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- PLSe4
- Outcome
- root growth and leaf vascular patterninge4
- Qualifier
- auxin-cytokinin homeostasise4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Gene of Arabidopsis”
e4“We propose that PLS is required for correct auxin-cytokinin homeostasis to modulate root growth and leaf vascular patterning”
The same questions in other papers
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See all 12 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.