Abstract
SUMMARY Members of the GRAS family of transcriptional regulators have been implicated in the control of plant growth and development, and in the interaction of plants with symbiotic bacteria. Here we examine the complexity of the GRAS gene family in tomato (Solanum lycopersicum) and investigate its role in disease resistance and mechanical stress. A large number of tomato ESTs corresponding to GRAS transcripts were retrieved from the public database and assembled in 17 contigs of putative genes. Expression analysis of these genes by real-time RT-PCR revealed that six SlGRAS transcripts accumulate during the onset of disease resistance to Pseudomonas syringae pv. tomato. Further analysis of two selected family members showed that their transcripts preferentially accumulate in tomato plants in response to different avirulent bacteria or to the fungal elicitor EIX, and their expression kinetics correlate with the appearance of the hypersensitive response. In addition, transcript levels of eight SlGRAS genes, including all the Pseudomonas-inducible family members, increased in response to mechanical stress much earlier than upon pathogen attack. Accumulation of SlGRAS transcripts following mechanical stress was in part dependent on the signalling molecule jasmonic acid. Remarkably, suppression of SlGRAS6 gene expression by virus-induced gene silencing impaired tomato resistance to P. syringae pv. tomato. These results support a function for GRAS transcriptional regulators in the plant response to biotic and abiotic stress.
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Claims 8
Claim · model-written summary
Tomato GRAS ESTs assembled into 17 putative gene contigs.
- Taxon
- tomato
Solanum lycopersicume1 - Gene
- GRASe0
- Outcome
- contigs of putative genese0
- Value
- 17 contigse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A large number of tomato ESTs corresponding to GRAS transcripts were retrieved from the public database and assembled in 17 contigs of putative genes”
e1“tomato (Solanum lycopersicum)”
Claim · model-written summary
Six SlGRAS transcripts accumulated at the onset of resistance to Pseudomonas syringae pv. tomato.
- Taxon
- tomatoSolanum lycopersicume1
- Interactor
- Pseudomonas syringae pv. tomato
Pseudomonas syringae pv. tomatoe0pathogen - Gene
- SlGRASe0
- Outcome
- SlGRAS transcripts accumulatee0
- Value
- sixe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“six SlGRAS transcripts accumulate during the onset of disease resistance to Pseudomonas syringae pv. tomato”
e1“tomato (Solanum lycopersicum)”
Claim · model-written summary
Two selected GRAS members accumulated transcripts after avirulent bacterial challenge.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- different avirulent bacteriae0
- Interactor
- avirulent bacteriae0pathogen
- Gene
- transcriptse2
- Outcome
- transcriptse3
- Direction
- accumulatee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their transcripts preferentially accumulate in tomato plants in response to different avirulent bacteria”
e1“tomato (Solanum lycopersicum)”
e2“their transcripts preferentially accumulate in tomato plants in response to different avirulent bacteria or to the fungal elicitor EIX”
e3“their transcripts preferentially accumulate in tomato plants in response to different avirulent bacteria or to the fungal elicitor EIX”
e4“their transcripts preferentially accumulate in tomato plants in response to different avirulent bacteria or to the fungal elicitor EIX”
Claim · model-written summary
Two selected GRAS members accumulated transcripts after EIX treatment.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- fungal elicitor EIXe0
- Gene
- transcriptse2
- Outcome
- transcriptse3
- Direction
- accumulatee4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“or to the fungal elicitor EIX, and their expression kinetics correlate with the appearance of the hypersensitive response”
e1“tomato (Solanum lycopersicum)”
e2“their transcripts preferentially accumulate in tomato plants in response to different avirulent bacteria or to the fungal elicitor EIX”
e3“their transcripts preferentially accumulate in tomato plants in response to different avirulent bacteria or to the fungal elicitor EIX”
e4“their transcripts preferentially accumulate in tomato plants in response to different avirulent bacteria or to the fungal elicitor EIX”
Claim · model-written summary
Their expression kinetics correlated with hypersensitive response appearance.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- expression kineticse0
- Outcome
- appearance of the hypersensitive responsee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their expression kinetics correlate with the appearance of the hypersensitive response”
e1“tomato (Solanum lycopersicum)”
Claim · model-written summary
Eight SlGRAS transcript levels increased under mechanical stress earlier than after pathogen attack.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- mechanical stresse0
- Gene
- SlGRAS genese0
- Outcome
- transcript levelse0
- Direction
- increasede0
- Value
- eighte0
- Comparator
- pathogen attacke0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcript levels of eight SlGRAS genes, including all the Pseudomonas-inducible family members, increased in response to mechanical stress much earlier than upon pathogen attack”
e1“tomato (Solanum lycopersicum)”
Claim · model-written summary
Jasmonic acid partly mediated mechanical-stress-induced SlGRAS transcript accumulation.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- SlGRASe0
- Compound
- jasmonic acide0
- Outcome
- Accumulation of SlGRAS transcriptse0
- Qualifier
- in part dependente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Accumulation of SlGRAS transcripts following mechanical stress was in part dependent on the signalling molecule jasmonic acid”
e1“tomato (Solanum lycopersicum)”
Claim · model-written summary
Silencing SlGRAS6 impaired resistance to P. syringae pv. tomato.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- virus-induced gene silencinge0
- Interactor
- P. syringae pv. tomatoPseudomonas syringae pv. tomatoe0pathogen
- Gene
- SlGRAS6e0
- Outcome
- tomato resistancee0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suppression of SlGRAS6 gene expression by virus-induced gene silencing impaired tomato resistance to P. syringae pv. tomato”
e1“tomato (Solanum lycopersicum)”
The same questions in other papers
loss of function · Pseudomonas syringae pv. tomato · Solanum lycopersicum (tomato)Positive and negative results
5 papers in the same direction as this paper · 4 in the opposite direction
- negative
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6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.