Abstract
The tomato Pto kinase confers resistance to the causative agent of bacterial speck disease, Pseudomonas syringae pv. tomato, by recognizing the pathogen effector proteins AvrPto or AvrPtoB. Pto-mediated resistance requires multiple signal transduction pathways and has been shown to activate many defense responses including an oxidative burst, rapid changes in the expression of over 400 genes, and localized cell death. We have tested the role in Pto-mediated resistance in tomato of a set of 21 genes from other species known to be involved in defense-related signaling. Expression of each gene was suppressed by virus-induced gene silencing (VIGS) and the effect on disease symptoms and bacterial growth during the tomato-Pseudomonas incompatible interaction was determined. We found that Pto-mediated resistance was compromised by silencing of genes encoding two mitogen-activated protein (MAP) kinase kinases, MEK1 and MEK2, two MAP kinases, NTF6 and wound-induced protein kinase (WIPK), a key regulator of systemic acquired resistance (SAR), NPR1, and two transcription factors, TGA1a and TGA2.2. A lesser impact on Pto-mediated resistance was observed in plants silenced for RAR1 and COI1. The identification of nine genes that play a role in resistance to bacterial speck disease both advances our knowledge of Pto signal transduction and demonstrates the conservation of many defense signaling components among diverse plant species.
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Claims 5
Claim · model-written summary
Silencing MEK1 and MEK2 compromised tomato Pto-mediated resistance.
- Taxon
- tomato
Solanum lycopersicume1 - Treatment
- silencinge0
- Interactor
- Pseudomonas syringae pv. tomato
Pseudomonas syringae pv. tomatoe2pathogen - Gene
- MEK1 and MEK2e0
- Outcome
- Pto-mediated resistancee0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pto-mediated resistance was compromised by silencing of genes encoding two mitogen-activated protein (MAP) kinase kinases, MEK1 and MEK2”
e1“The tomato Pto kinase”
e2“Pseudomonas syringae pv. tomato”
Claim · model-written summary
Silencing NTF6 and WIPK compromised tomato Pto-mediated resistance.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- silencinge0
- Interactor
- Pseudomonas syringae pv. tomatoPseudomonas syringae pv. tomatoe2pathogen
- Gene
- NTF6 and wound-induced protein kinase (WIPK)e0
- Outcome
- Pto-mediated resistancee0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pto-mediated resistance was compromised by silencing of genes encoding two mitogen-activated protein (MAP) kinase kinases, MEK1 and MEK2, two MAP kinases, NTF6 and wound-induced protein kinase (WIPK)”
e1“The tomato Pto kinase”
e2“Pseudomonas syringae pv. tomato”
Claim · model-written summary
Silencing NPR1 compromised tomato Pto-mediated resistance.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- silencinge0
- Interactor
- Pseudomonas syringae pv. tomatoPseudomonas syringae pv. tomatoe2pathogen
- Gene
- NPR1e0
- Outcome
- Pto-mediated resistancee0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pto-mediated resistance was compromised by silencing of genes encoding two mitogen-activated protein (MAP) kinase kinases, MEK1 and MEK2, two MAP kinases, NTF6 and wound-induced protein kinase (WIPK), a key regulator of systemic acquired resistance (SAR), NPR1”
e1“The tomato Pto kinase”
e2“Pseudomonas syringae pv. tomato”
Claim · model-written summary
Silencing TGA1a and TGA2.2 compromised tomato Pto-mediated resistance.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- silencinge0
- Interactor
- Pseudomonas syringae pv. tomatoPseudomonas syringae pv. tomatoe2pathogen
- Gene
- TGA1a and TGA2.2e0
- Outcome
- Pto-mediated resistancee0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Pto-mediated resistance was compromised by silencing of genes encoding two mitogen-activated protein (MAP) kinase kinases, MEK1 and MEK2, two MAP kinases, NTF6 and wound-induced protein kinase (WIPK), a key regulator of systemic acquired resistance (SAR), NPR1, and two transcription factors, TGA1a and TGA2.2.”
e1“The tomato Pto kinase”
e2“Pseudomonas syringae pv. tomato”
Claim · model-written summary
Silencing RAR1 and COI1 had a lesser impact on tomato Pto-mediated resistance.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- silencede0
- Interactor
- Pseudomonas syringae pv. tomatoPseudomonas syringae pv. tomatoe2pathogen
- Gene
- RAR1 and COI1e0
- Outcome
- Pto-mediated resistancee0
- Direction
- lessere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A lesser impact on Pto-mediated resistance was observed in plants silenced for RAR1 and COI1.”
e1“The tomato Pto kinase”
e2“Pseudomonas syringae pv. tomato”
The same questions in other papers
loss of function · Pseudomonas syringae pv. tomato · Solanum lycopersicum (tomato)Positive and negative results
5 papers in the same direction as this paper · 4 in the opposite direction
- positive
- positive
- negative
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.