Abstract
Immunohistological analyses for sucrose phosphate synthase (SPS) show that the protein is localized in both bundle-sheath cells (BS) and mesophyll cells (M) in maize (Zea mays) leaves. In young leaves, SPS protein was predominantly in the BS, whereas mature leaves showed nearly equal levels of signal in both BS and M. A cell-type-specific response was also seen in light and dark treatments. Dark treatments led to reduced signal in M; however, little or no change was detected in BS. We suggest that SPS in BS is engaged in sucrose biosynthesis by both photoassimilatory and starch turnover reactions in maize leaves. In addition, we suggest that the enzyme in BS may play a major role in the early biosynthesis of sucrose in young leaves. These cell-specific changes in expression in situ were in agreement with the estimates of extractable enzyme activity from isolated BS and M of mature leaves (R. Ohsugi, S.C. Huber [1987] Plant Physiol 84: 1096-1101). In contrast, western blot analyses did not show any significant changes in the levels of SPS protein in either young or mature leaves subsequent to similar dark treatments. It is interesting that the northern blot analyses indicate that the steady-state levels of SPS transcripts were markedly reduced after dark treatments of > 12 h. Overall, our results indicate that Sps gene expression in maize leaf is modulated at multiple levels of controls by both developmental and environmental factors.
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Claims 7
Claim · model-written summary
SPS protein occurs in bundle-sheath and mesophyll cells of maize leaves.
- Organ / stage
- leavese0
- Gene
- sucrose phosphate synthase (SPS)e0
- Outcome
- localized in both bundle-sheath cells (BS) and mesophyll cells (M)e0
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Immunohistological analyses for sucrose phosphate synthase (SPS) show that the protein is localized in both bundle-sheath cells (BS) and mesophyll cells (M) in maize (Zea mays) leaves.”
Claim · model-written summary
In young leaves, SPS protein is predominantly in bundle-sheath cells.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In young leaves, SPS protein was predominantly in the BS”
t0“Maize Leaf”
Claim · model-written summary
In mature leaves, SPS signal is nearly equal in bundle-sheath and mesophyll cells.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“mature leaves showed nearly equal levels of signal in both BS and M.”
t0“Maize Leaf”
Claim · model-written summary
Darkness reduced SPS signal in mesophyll cells.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Dark treatments led to reduced signal in M”
t0“Sucrose Phosphate Synthase Expression at the Cell and Tissue Level Is Coordinated with Sucrose Sink-to-Source Transitions in Maize Leaf”
Claim · model-written summary
Darkness produced little or no SPS signal change in bundle-sheath cells.
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“little or no change was detected in BS.”
t0“Sucrose Phosphate Synthase Expression at the Cell and Tissue Level Is Coordinated with Sucrose Sink-to-Source Transitions in Maize Leaf”
Claim · model-written summary
Western blots showed no significant SPS protein change after darkness in young or mature leaves.
- Treatment
- dark treatmentse0
- Gene
- SPSe0
- Outcome
- levels of SPS proteine0
- Direction
- did not show any significant changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“western blot analyses did not show any significant changes in the levels of SPS protein in either young or mature leaves subsequent to similar dark treatments.”
t0“Maize Leaf”
Claim · model-written summary
SPS transcripts were markedly reduced after more than 12 hours of darkness.
- Treatment
- dark treatments of > 12 he0
- Gene
- SPSe0
- Outcome
- steady-state levels of SPS transcriptse0
- Direction
- markedly reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“steady-state levels of SPS transcripts were markedly reduced after dark treatments of > 12 h.”
t0“Maize Leaf”
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