Abstract
Both light and developmental stimuli are directly involved in the regulation of plant gene expression. In maize, activation of the anthocyanin pathway represents an excellent model system for studying the interactions between an external factor, such as light, and internal factors that regulate plant and seed development. By analyzing in detail the aleurone and pericarp seed layers, different developmental windows for light have been found in the two tissues[mdash]the former in the advanced stages of development and the latter in the early stages of seed development. Transcriptional control of the structural genes involved in anthocyanin deposition within the pericarp is known to be exerted by the Sn and pl genes, whereas the aleurone is controlled by the R and C1 regulatory genes. By using in situ hybridization analysis, we detected tissue-specific expression of Sn and R in the seed layers, revealing a correlation between structural gene activation and anthocyanin accumulation. In addition, RNA gel blot analysis revealed that Sn expression is enhanced by light, whereas the R gene expression is not. However, the light-induced expression of the myb-type genes C1 and pl, detected by reverse transcriptase-polymerase chain reaction, was found to be the limiting factor for conferring the developmental competence of the pericarp and the aleurone layers to light responsiveness.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
The aleurone was light responsive in advanced developmental stages.
- Organ / stage
- aleuronee1
- Outcome
- developmental windows for lighte0
- Qualifier
- advanced stages of developmente0
Zea maysOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“different developmental windows for light have been found in the two tissues[mdash]the former in the advanced stages of development and the latter in the early stages of seed development”
e1“the aleurone and pericarp seed layers”
e2“In maize”
Claim · model-written summary
The pericarp was light responsive early in seed development.
- Organ / stage
- pericarpe1
- Outcome
- developmental windows for lighte0
- Qualifier
- early stages of seed developmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“different developmental windows for light have been found in the two tissues[mdash]the former in the advanced stages of development and the latter in the early stages of seed development”
e1“the aleurone and pericarp seed layers”
e2“In maize”
Claim · model-written summary
Sn showed tissue-specific expression in maize seed layers, correlated with structural gene activation and anthocyanin accumulation.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we detected tissue-specific expression of Sn and R in the seed layers, revealing a correlation between structural gene activation and anthocyanin accumulation”
e1“In maize”
Claim · model-written summary
R showed tissue-specific expression in maize seed layers, correlated with structural gene activation and anthocyanin accumulation.
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we detected tissue-specific expression of Sn and R in the seed layers, revealing a correlation between structural gene activation and anthocyanin accumulation”
e1“In maize”
Claim · model-written summary
Light enhanced Sn expression.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sn expression is enhanced by light”
e1“In maize”
Claim · model-written summary
Light did not enhance R gene expression.
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“whereas the R gene expression is not”
e1“Sn expression is enhanced by light”
e2“In maize”
Claim · model-written summary
The authors identify light-induced C1 expression as limiting developmental competence of the aleurone and pericarp to respond to light.
- Gene
- C1e0
- Outcome
- developmental competence of the pericarp and the aleurone layers to light responsivenesse0
- Qualifier
- light-induced expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the light-induced expression of the myb-type genes C1 and pl, detected by reverse transcriptase-polymerase chain reaction, was found to be the limiting factor for conferring the developmental competence of the pericarp and the aleurone layers to light responsiveness”
e1“In maize”
Claim · model-written summary
The authors identify light-induced pl expression as limiting developmental competence of the aleurone and pericarp to respond to light.
- Gene
- ple0
- Outcome
- developmental competence of the pericarp and the aleurone layers to light responsivenesse0
- Qualifier
- light-induced expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the light-induced expression of the myb-type genes C1 and pl, detected by reverse transcriptase-polymerase chain reaction, was found to be the limiting factor for conferring the developmental competence of the pericarp and the aleurone layers to light responsiveness”
e1“In maize”
The same questions in other papers
light · molecular: expression · Zea mays (maize)Positive and negative results
17 papers in the same direction as this paper · 11 in the opposite direction · 1 reporting no difference from an alternative
- positive
- positive
- positive
26 papers more in this group
See all 30 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.