Abstract
Plants disease resistance (R) genes encode specialized receptors that are quantitative, rate-limiting defense regulators. R genes must be expressed at optimum levels to function properly. If expression is too low, downstream defense responses are not activated efficiently. Conversely, overexpression of R genes can trigger autoactivation of defenses with deleterious consequences for the plant. Little is known about R gene regulation, particularly under defense-inducing conditions. We examined regulation of the Arabidopsis thaliana gene RPP8 (resistance to Hyaloperonospora arabidopsidis, isolate Emco5). RPP8 was induced in response to challenge with H. arabidopsidis or application of salicylic acid, as shown with RPP8-Luciferase transgenic plants and quantitative reverse-transcription polymerase chain reaction of endogenous alleles. The RPP1 and RPP4 genes were also induced by H. arabidopsidis and salicylic acid, suggesting that some RPP genes are subject to feedback amplification. The RPP8 promoter contains three W box cis elements. Site-directed mutagenesis of all three W boxes greatly diminished RPP8 basal expression, inducibility, and resistance in transgenic plants. Motif searches indicated that the W box is the only known cis element that is statistically overrepresented in Arabidopsis nucleotide-binding leucine-rich repeat promoters. These results indicate that WRKY transcription factors can regulate expression of surveillance genes at the top of the defense-signaling cascade.
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Claims 8
Claim · model-written summary
RPP8 was induced by H. arabidopsidis.
- Taxon
- Arabidopsis
Arabidopsist0 - Treatment
- challenge with H. arabidopsidise0
- Interactor
- H. arabidopsidis
Hyaloperonospora arabidopsidise0pathogen - Gene
- RPP8e0
- Outcome
- RPP8e0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RPP8 was induced in response to challenge with H. arabidopsidis or application of salicylic acid”
t0“Arabidopsis”
Claim · model-written summary
RPP8 was induced by salicylic acid.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RPP8 was induced in response to challenge with H. arabidopsidis or application of salicylic acid”
t0“Arabidopsis”
Claim · model-written summary
RPP1 and RPP4 were induced by pathogen challenge.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- H. arabidopsidise0
- Interactor
- H. arabidopsidisHyaloperonospora arabidopsidise0pathogen
- Outcome
- RPP1 and RPP4 genese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The RPP1 and RPP4 genes were also induced by H. arabidopsidis and salicylic acid”
t0“Arabidopsis”
Claim · model-written summary
RPP1 and RPP4 were induced by salicylic acid.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- salicylic acide0
- Outcome
- RPP1 and RPP4 genese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The RPP1 and RPP4 genes were also induced by H. arabidopsidis and salicylic acid”
t0“Arabidopsis”
Claim · model-written summary
The RPP8 promoter has three W box cis elements.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- RPP8e0
- Outcome
- three W box cis elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The RPP8 promoter contains three W box cis elements”
t0“Arabidopsis”
Claim · model-written summary
Mutating all three W boxes diminished basal RPP8 expression, inducibility and resistance.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- Site-directed mutagenesis of all three W boxese0
- Gene
- RPP8e0
- Outcome
- RPP8 basal expression, inducibility, and resistancee0
- Direction
- diminishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Site-directed mutagenesis of all three W boxes greatly diminished RPP8 basal expression, inducibility, and resistance in transgenic plants”
t0“Arabidopsis”
Claim · model-written summary
W box was the sole known overrepresented cis element in the studied promoters.
- Taxon
- ArabidopsisArabidopsise0
- Outcome
- W box is the only known cis element that is statistically overrepresentede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“W box is the only known cis element that is statistically overrepresented in Arabidopsis nucleotide-binding leucine-rich repeat promoters”
Claim · model-written summary
WRKY factors can regulate surveillance gene expression.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- WRKY transcription factorse0
- Outcome
- expression of surveillance genese0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WRKY transcription factors can regulate expression of surveillance genes at the top of the defense-signaling cascade”
t0“Arabidopsis”
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