Abstract
Goldsworthy, A. and Rathore, K. S. 1985. The electrical control of growth in plant tissue cultures: The polar transport of auxin.—J. exp. Bot. 36: 1134–1141. The reasons for a many-fold stimulation of shoot formation and a 60–70% stimulation of growth in tobacco callus caused by passing a very weak electric current (1 or 2 μA) between the callus and the culture medium have been investigated. The stimulation of callus growth occurred only when the callus was made negative to the medium and then only when IAA was added. It was abolished, even in the presence of IAA, by the addition of TIBA which is an inhibitor of polar auxin transport, and also when the IAA was substituted by either IAN or the synthetic auxin 2,4-D, neither of which show significant polar transport. This suggests that the electrical treatment may have aligned the physiological polarities of the callus cells so as to promote the polar transport of IAA into the tissue when the callus was negative to the medium. If so, the enhanced shoot formation may have been due to the parallel orientation of the growth axes of individual cells so as to make the production of organforming meristems more likely. The mechanism of the effect and its relationship to the natural forces controlling differentiation is discussed.
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Claims 7
Claim · model-written summary
A weak electric current passed between callus and medium produced a many-fold stimulation of shoot formation in tobacco callus.
- Taxon
- tobacco
Nicotiana tabacume1 - Organ / stage
- calluse1
- Treatment
- passing a very weak electric current (1 or 2 μA) between the callus and the culture mediume1
- Outcome
- shoot formatione1
- Direction
- stimulatione1
- Value
- many-folde1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The reasons for a many-fold stimulation of shoot formation and a 60–70% stimulation of growth in tobacco callus caused by passing a very weak electric current (1 or 2 μA) between the callus and the culture medium have been investigated”
Claim · model-written summary
A weak electric current stimulated tobacco callus growth by 60–70%.
- Taxon
- tobaccoNicotiana tabacume1
- Organ / stage
- calluse1
- Treatment
- passing a very weak electric current (1 or 2 μA) between the callus and the culture mediume1
- Outcome
- growthe1
- Direction
- stimulatione1
- Value
- 60–70%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The reasons for a many-fold stimulation of shoot formation and a 60–70% stimulation of growth in tobacco callus caused by passing a very weak electric current (1 or 2 μA) between the callus and the culture medium have been investigated”
Claim · model-written summary
Stimulation of callus growth occurred only when the callus was negative to the medium and only when IAA was added.
- Taxon
- tobaccoNicotiana tabacume1
- Organ / stage
- calluse1
- Treatment
- the callus was made negative to the mediume2
- Outcome
- callus growthe2
- Direction
- stimulatione2
- Qualifier
- only when IAA was addede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The reasons for a many-fold stimulation of shoot formation and a 60–70% stimulation of growth in tobacco callus”
e2“The stimulation of callus growth occurred only when the callus was made negative to the medium and then only when IAA was added”
Claim · model-written summary
Addition of TIBA, an inhibitor of polar auxin transport, abolished the growth stimulation even in the presence of IAA.
- Taxon
- tobaccoNicotiana tabacume1
- Organ / stage
- calluse1
- Treatment
- the addition of TIBAe3
- Outcome
- callus growthe2
- Direction
- abolishede3
- Comparator
- in the presence of IAAe3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The reasons for a many-fold stimulation of shoot formation and a 60–70% stimulation of growth in tobacco callus”
e2“The stimulation of callus growth occurred only when the callus was made negative to the medium and then only when IAA was added”
e3“It was abolished, even in the presence of IAA, by the addition of TIBA which is an inhibitor of polar auxin transport”
Claim · model-written summary
Substituting IAA with IAN or 2,4-D abolished the stimulation of callus growth in tobacco.
- Taxon
- tobaccoNicotiana tabacume1
- Organ / stage
- calluse1
- Treatment
- the IAA was substituted by either IAN or the synthetic auxin 2,4-De4
- Outcome
- callus growthe2
- Direction
- abolishede3
- Comparator
- IAAe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The reasons for a many-fold stimulation of shoot formation and a 60–70% stimulation of growth in tobacco callus”
e2“The stimulation of callus growth occurred only when the callus was made negative to the medium and then only when IAA was added”
e3“It was abolished, even in the presence of IAA, by the addition of TIBA which is an inhibitor of polar auxin transport”
e4“and also when the IAA was substituted by either IAN or the synthetic auxin 2,4-D”
Claim · model-written summary
The electrical treatment may have aligned callus cell polarities to promote polar transport of IAA into the tissue.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- polar transport of IAAe5
- Qualifier
- aligned the physiological polarities of the callus cellse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The reasons for a many-fold stimulation of shoot formation and a 60–70% stimulation of growth in tobacco callus”
e5“This suggests that the electrical treatment may have aligned the physiological polarities of the callus cells so as to promote the polar transport of IAA into the tissue when the callus was negative to the medium”
Claim · model-written summary
Enhanced shoot formation may be due to parallel orientation of the growth axes of individual callus cells.
- Taxon
- tobaccoNicotiana tabacume1
- Outcome
- shoot formatione6
- Qualifier
- parallel orientation of the growth axes of individual cellse6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The reasons for a many-fold stimulation of shoot formation and a 60–70% stimulation of growth in tobacco callus”
e6“the enhanced shoot formation may have been due to the parallel orientation of the growth axes of individual cells”
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