Abstract
The glutamine synthetase (GS) gene family in pea (Pisum sativum) consists of four nuclear genes encoding distinct isoenzymes. Molecular studies have show that the GS2 gene encoding chloroplast-localized GS is expected in specific cell types and is regulated by diverse factors such as light and photorespiration. Here, we present the nucleotide sequence of the pea GS2 gene promoter. To identify the elements involved in regulation of GS2 expression, GS2 promoter-deletion analyses were performed using GS2-GUS fusions in tobacco (Nicotiana tabacum). This analysis revealed that the GS2 transit peptide is not required for mesophyll cell-specific expression of beta-glucuronidase (GUS). GUS activity was induced 2- to 4-fold in light-grown versus etiolated T1 seedlings. However, high levels of GUS activity were observed in etiolated seedlings. This observation demonstrated that regulation of expression of GS2, a nonphotosynthetic light-regulated gene, involves additional factors. A 323-bp GS2 promoter sequence is sufficient to confer light regulation to the GUS reporter gene in leaves of mature transgenic tobacco. Light-regulated expression of this pea gene promoter is observed in both tobacco and Arabidopsis, suggesting that the regulatory elements are conserved. Gel-shift analysis detected DNA-protein complexes formed with potential transcription elements within this short, light-responsive GS2 promoter fragment.
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Claims 8
Claim · model-written summary
The pea GS2 promoter nucleotide sequence was presented.
- Taxon
- pea
Lathyrus oleraceuse0 - Gene
- GS2e0
- Outcome
- nucleotide sequence of the pea GS2 gene promotere0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we present the nucleotide sequence of the pea GS2 gene promoter”
Claim · model-written summary
GS2 transit peptide was not required for mesophyll-specific GUS expression in tobacco.
- Taxon
- tobacco
Nicotiana tabacumt0 - Gene
- GS2e0
- Outcome
- mesophyll cell-specific expression of beta-glucuronidase (GUS)e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the GS2 transit peptide is not required for mesophyll cell-specific expression of beta-glucuronidase (GUS)”
t0“tobacco (Nicotiana tabacum)”
Claim · model-written summary
Light-grown T1 seedlings had 2- to 4-fold induced GUS activity relative to etiolated seedlings.
- Taxon
- tobaccoNicotiana tabacumt0
- Treatment
- light-growne0
- Outcome
- GUS activitye0
- Direction
- inducede0
- Value
- 2- to 4-folde0
- Comparator
- etiolated T1 seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GUS activity was induced 2- to 4-fold in light-grown versus etiolated T1 seedlings”
t0“tobacco (Nicotiana tabacum)”
Claim · model-written summary
Etiolated seedlings had high GUS activity.
- Taxon
- tobaccoNicotiana tabacumt0
- Organ / stage
- etiolated seedlingse0
- Outcome
- GUS activitye0
- Direction
- high levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“high levels of GUS activity were observed in etiolated seedlings”
t0“tobacco (Nicotiana tabacum)”
Claim · model-written summary
A 323-bp GS2 promoter sequence conferred light regulation to GUS in mature tobacco leaves.
- Taxon
- tobaccoNicotiana tabacume0
- Organ / stage
- leavese0
- Gene
- GS2e0
- Outcome
- light regulation to the GUS reporter genee0
- Value
- 323-bpe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A 323-bp GS2 promoter sequence is sufficient to confer light regulation to the GUS reporter gene in leaves of mature transgenic tobacco”
Claim · model-written summary
The pea promoter had light-regulated expression in tobacco.
- Taxon
- tobaccoNicotiana tabacume0
- Outcome
- Light-regulated expressione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Light-regulated expression of this pea gene promoter is observed in both tobacco and Arabidopsis”
Claim · model-written summary
The pea promoter had light-regulated expression in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Outcome
- Light-regulated expressione0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Light-regulated expression of this pea gene promoter is observed in both tobacco and Arabidopsis”
Claim · model-written summary
Gel shift detected DNA-protein complexes at potential elements in the responsive GS2 promoter fragment.
- Taxon
- peaLathyrus oleraceust0
- Gene
- GS2e0
- Outcome
- DNA-protein complexes formed with potential transcription elementse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gel-shift analysis detected DNA-protein complexes formed with potential transcription elements within this short, light-responsive GS2 promoter fragment”
t0“pea (Pisum sativum)”
The same questions in other papers
light · molecular: expression · Nicotiana tabacum (tobacco)Positive and negative results
8 papers in the same direction as this paper · 5 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- negative
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.